The stimulation of ACTH release from anterior pituitary cells by corticotropin-releasing factor (CRF) is mediated specific, high affinity receptors with a Ka 10(9) M-1 for ovine CRF. relationship between secretion and CRF receptor activation was analyzed in normal adrenalectomized rats comparison changes adenylate cyclase activity. marked increase plasma levels that occurred after adrenalectomy (from 71 to 478 pg/ml 4 days) accompanied progressive decrease concentration [by 29 +/- 1%, 75 2%,...

The rise in circulating ACTH levels after adrenalectomy the rat is associated with a decrease CRF receptorbinding capacity anterior pituitary. To investigate role of increased hypothalamic release on pituitary receptor regulation withdrawal glucocorticoid feedback by adrenalectomy, effects chronic infusion and lesions medial basal hypothalamus were studied rat. Subcutaneous at 10, 25, 50, 100 ng/min for 48 h intact rats caused dose-dependent increases plasma from control value 32.1 ± 4.3 to...

Milk oligosaccharides (MOs) are complex carbohydrates with multifunctional health benefits for the neonate. Poor reproductive performance in primiparous gilts limits their productivity. Changes structure and abundance of porcine MO (PMOs) through lactation parity remains unknown may explain superior new-born growth litters from multiparous sows relative to gilts. We report 55 PMOs structures, which 25 new (17 sialylated 8 neutral). Their incidence gilt sow colostrum was almost identical (53...

The present study was conducted to determine the pattern of incorporation dietary EPA and docosahexaenoic acid (DHA) into milk, evaluate consequent changes in milk fat composition sensory characteristics. Fourteen multiparous cows early lactation were divided two groups offered supplements for 10 d. While individual stalls after each morning milking, one group a mixture rumen-protected tuna oil (RPTO)-soyabean supplement (2 kg; 30:70, w/w; +RPTO) second basal ration without RPTO (-RPTO)....

The receptor-binding properties and in vitro biological effects of a highly active gonadotropin-releasing hormone (GnRH) antagonist, [N-acetyl-D-p-chloro-Phe1,2D-Trp3,D-Lys6,D-Ala10]GnRH, were compared with those the GnRH superagonist analog, [D-Ala6] des-Gly10-GnRH-N-ethylamide. In rat pituitary particles isolated cells, 125I-labeled antagonist showed saturable high-affinity binding (Ka v 8.4 +/- 1.4 X 10(9) M-1) to same receptor sites which bound agonist. rate dissociation receptor-bound...

The potential genetic and economic advantage of marker-assisted selection for enhanced production in dairy cattle has provided an impetus to conduct numerous genome scans order identify associations between DNA markers future productive potential. One area focus been a quantitative trait locus on bovine chromosome 6 (BTA6) found be associated with milk yield, protein fat percentage, which subsequently fine-mapped six positional candidate genes. Subsequent investigations have yet resolve the...

Sialic acids (Sias) are acidic monosaccharides and red meat is a notable dietary source of Sia for humans. Among the Sias, N-acetylneuraminic acid (Neu5Ac) 2-keto-3-deoxy-D-glycero-D-galacto-2-nonulosonic (KDN) play multiple roles in immunity brain cognition. On other hand, N-glycolylneuraminic (Neu5Gc) non-human capable potentiating cancer inflammation human body. However, their expression within animal kingdom remains unknown. We determined Neu5Ac KDN skeletal muscle organs across range (n...

Receptors for CRH were identified in the pituitary gland of several primate species, and their binding characteristics compared to ability elicit ACTH cAMP responses vitro. Autoradiographic analysis [125I]Tyr-ovine frozen sections revealed receptors intermediate anterior lobes human, marmoset, cynomolgus monkey pituitaries. In monkey, a high density was present throughout lobes. human pituitary, concentrated anteromedial portion gland, whereas dense lobe scattered as clusters lobe....

Lactoferrin (LF), a sialylated iron-binding glycoprotein, performs multiple beneficial functions including modulating immunity and improves neurodevelopment, health growth performance. Maternal LF intervention for gilts (first parity sows) on the performance of their offspring remains unknown. In current study were fed with commercial pig feed supplemented 1g /day (treatment group) or milk casein/day (control from day 1 post mating throughout pregnancy lactation about 135 days. The...

Summary An in vitro bovine mammosphere model was characterized for use lactational biology studies using a functional genomics approach. Primary mammary epithelial cells cultured on basement membrane, Matrigel, formed three‐dimensional alveoli‐like structures or mammospheres. Gene expression profiling during formation by high‐density microarray analysis indicated that mammospheres underwent similar molecular and cellular processes to developing alveoli the gland. profiles genes involved milk...

Acute phase and inflammatory responses are triggered by a variety of intrinsic extrinsic stressors that come at cost through suppressing the normal function tissues organs domestic animals. Recently, with growing attention placed on global warming animal welfare, there has been an increased interest in improving our understanding relationships between different classes stress, expression acute proteins (APPs), stress-related endocrine system immunomodulation. Immune is compromised all forms...