A5023813406- Developmental Biology and Gene Regulation
- Neurobiology and Insect Physiology Research
- TGF-β signaling in diseases
- Cellular Mechanics and Interactions
- Hippo pathway signaling and YAP/TAZ
- Circadian rhythm and melatonin
- Physiological and biochemical adaptations
- Silk-based biomaterials and applications
- Olfactory and Sensory Function Studies
- Muscle Physiology and Disorders
- Neuroscience and Neuropharmacology Research
- Marine Biology and Environmental Chemistry
- Flowering Plant Growth and Cultivation
- Genetic and Kidney Cyst Diseases
- Animal Genetics and Reproduction
- Insect Resistance and Genetics
- Cell Adhesion Molecules Research
- Evolution and Science Education
- Memory and Neural Mechanisms
- Ubiquitin and proteasome pathways
- Pluripotent Stem Cells Research
- Genomics and Chromatin Dynamics
- Protein Kinase Regulation and GTPase Signaling
- Connective tissue disorders research
- RNA Research and Splicing
Howard Hughes Medical Institute
1996-2020
Janelia Research Campus
2018-2020
Helix (United States)
2018
University of Sussex
2005-2016
The Francis Crick Institute
2015
The Honourable Society of Lincoln's Inn
2012-2015
Cancer Research UK
2012
Brown University
2001
Princeton University
1996-2000
John Brown University
1998
ABSTRACT decapentaplegic (dpp) is a zygotically expressed gene encoding TGF-β-related ligand that necessary for dorsal-ventral patterning in the Drosophila embryo. We show here dpp an integral part of gradient specifies many different cell fates via intercellular signalling. There graded requirement activity early embryo: high levels specify amnioserosa, while progressively lower dorsal and lateral ectoderm. This potential to fate highly dosage sensitive. In wildtype embryo, increasing can...
We have analyzed the contributions made by maternal and zygotic genes to establishment of expression patterns four patterning genes: decapentaplegic (dpp), zerknüllt (zen), twist (twi), snail (sna). All these are initially expressed either dorsally or ventrally in segmented region embryo, at poles. In correct depends on cues from morphogen dorsal (dl). The dl gradient appears be interpreted three levels: cells express dpp zen, but not twi sna; lateral lack all genes; ventral sna, zen....
okra ( okr ), spindle-B spnB and spindle-D spnD ) are three members of a group female sterile loci that produce defects in oocyte egg morphology, including variable dorsal–ventral the eggshell embryo, anterior–posterior follicle cell epithelium oocyte, abnormalities nuclear morphology. Many these phenotypes reflect grk-Egfr signaling processes, can be accounted for by failure to accumulate wild-type levels Gurken Fs(1)K10. We have cloned , show encodes Drosophila homolog yeast DNA-repair...
Animals employ diverse learning rules and synaptic plasticity dynamics to record temporal statistical information about the world. However, molecular mechanisms underlying this diversity are poorly understood. The anatomically defined compartments of insect mushroom body function as parallel units associative learning, with different rates, memory decay flexibility (Aso Rubin, 2016). Here, we show that nitric oxide (NO) acts a neurotransmitter in subset dopaminergic neurons Drosophila . NO’s...
Highlights•The apical extracellular matrix protein Dumpy (Dp) is required for appendage shape•Dp anchors the epidermis to cuticle, generating tension during tissue contraction•Alteration of pattern Dp gives rise predictable changes in shape•Narrow (Nw), Tapered (Ta), and Lanceolate (Ll) affect shape by modulating DpSummaryHow tissues acquire their characteristic a fundamental unresolved question biology. While genes have been characterized that control local mechanical forces elongate...
Epithelial tissues can elongate in two dimensions by polarized cell intercalation, oriented division, or shape change, owing to local global actomyosin contractile forces acting the plane of tissue. In addition, epithelia undergo morphogenetic change three dimensions. We show that elongation wings and legs Drosophila involves a columnar-to-cuboidal reduces height expands width. Remodeling apical extracellular matrix Stubble protease basal MMP1/2 proteases induces wing leg elongation. Matrix...
The pattern of the large sensory bristles on notum Drosophila arises as a consequence expression achaete and scute genes. gene u-shaped encodes novel zinc finger that acts transregulator in dorsal region notum. Viable hypomorphic mutants display additional dorsocentral scutellar result from overexpression scute. In contrast, causes loss achaete–scute consequently bristles. effects appear to be mediated through enhancer sequences regulate at this site. are similar those class dominant alleles...
Abstract Insect wing shapes are remarkably diverse and the combination of shape kinematics determines both aerial capabilities power requirements. However, contribution any specific morphological feature to performance is not known. Using targeted RNA interference modify far beyond natural variation found within population a single species, we show direct effect on flight that can be explained by physical modelling novel geometry. Our data altering expression gene significantly enhance...
The Drosophila BMP5/6/7/8 homolog, glass bottom boat (gbb), has been shown to be involved in proliferation and vein patterning the wing disk. To better understand roles for gbb development, as well its relationship with BMP2/4 homolog decapentaplegic (dpp), we have used clonal analysis define functional foci of during development. Our results show that both local long-range functions disk coincide spatially functionally established dpp, suggesting BMPs contribute same processes Indeed,...
Dystroglycan localizes to the basal domain of epithelial cells and has been reported play a role in apical-basal polarity. Here, we show that null mutant follicle have normal polarity, but lose planar polarity their actin stress fibers, phenotype it shares with Dystrophin mutants. However, unlike mutants, mutants or its extracellular matrix ligand Perlecan under energetic stress. The maintenance requires activation Myosin II by cellular energy sensor AMPK. Starved activate AMPK normally, do...
Members of the Rho family small GTPases are required for many morphogenetic processes to shape animal body. The activity this is regulated in part by a class proteins known as RhoGTPase Activating Proteins (RhoGAPs) that catalyse conversion RhoGTPases their inactive state. In our search genes regulate Drosophila morphogenesis, we have isolated several lethal alleles crossveinless-c (cv-c). Molecular characterisation reveals cv-c encodes RhoGAP protein RhoGAP88C. During embryonic development,...
ABSTRACT Neural representations of head direction have been discovered in many species. A large body theoretical work has proposed that the dynamics associated with these is generated, maintained, and updated by recurrent network structures called ring attractors. We performed electron microscopy-based circuit reconstruction RNA profiling identified cell types heading system Drosophila melanogaster to directly determine underlying neural network. motifs hypothesized maintain representation...
Bone morphogenetic proteins (BMPs) are synthesized as proproteins that undergo proteolytic processing by furin/subtilisin proprotein convertases to release the active ligand. Here we study of BMP5/6/7/8 proteins, including Drosophila orthologs Glass Bottom Boat (Gbb) and Screw (Scw) human BMP7. Gbb Scw have three functional convertase cleavage sites; two between prodomain ligand domain, which call Main Shadow sites, one within prodomain, Pro site. In each site can be cleaved independently,...
Abstract We have identified the molecular lesions associated with six point mutations in Drosophila TGF-β homologue decapentaplegic (dpp). The sites of these define residues within both pro and ligand regions that are essential for dpp function vivo. While all affect highly conserved among superfamily members, phenotypic consequences different alleles quite distinct. Through an analysis mutant phenotypes, cuticle preparations probes, we assessed functional significance specific members...
In mammals, the Transforming Growth Factor-beta (TGF-beta) superfamily controls a variety of developmental processes. Drosophila, by contrast, single member superfamily, decapentaplegic (dpp) performs most TGF-beta functions. The complexity dpp functions is reflected in complex cis-regulatory sequences that flank gene. Dpp divided into three regions: Hin, including protein-coding exons; disk, 3' sequences; and shortvein (shv), noncoding exons 5' sequences. We analyzed structure region using...
Summary Animals employ multiple and distributed neuronal networks with diverse learning rules synaptic plasticity dynamics to record distinct temporal statistical information about the world. However, molecular mechanisms underlying this diversity are poorly understood. The anatomically defined compartments of insect mushroom body function as parallel units associative learning, different rates, memory decay flexibility (Aso & Rubin 2016). Here we show that nitric oxide (NO) acts a...