A5072153983- Developmental Biology and Gene Regulation
- Invertebrate Immune Response Mechanisms
- Hippo pathway signaling and YAP/TAZ
- Cellular transport and secretion
- Neurobiology and Insect Physiology Research
- Protist diversity and phylogeny
- DNA Repair Mechanisms
- Chromosomal and Genetic Variations
- RNA Research and Splicing
- Glycosylation and Glycoproteins Research
- Genomics and Chromatin Dynamics
- Plant Molecular Biology Research
- Microtubule and mitosis dynamics
- RNA modifications and cancer
- Silk-based biomaterials and applications
- Vascular Malformations Diagnosis and Treatment
- Acute Kidney Injury Research
- Circadian rhythm and melatonin
- Pulmonary Hypertension Research and Treatments
- Cerebrospinal fluid and hydrocephalus
- CRISPR and Genetic Engineering
- Viral Infections and Vectors
- Plant responses to water stress
- Physiological and biochemical adaptations
- Nitric Oxide and Endothelin Effects
Columbia University Irving Medical Center
2018-2024
Columbia University
2018-2023
University of Pennsylvania
2008-2015
Stanford University
2003-2011
Howard Hughes Medical Institute
1997-2011
Princeton University
1997-1999
ABSTRACT The Drosophila gene torpedo/Egfr (top/Egfr) encodes a homolog of the vertebrate Epidermal Growth Factor receptor. This receptor is required several times during life cycle fly for transmission developmental cues. During oogenesis, Top/Egfr activation establishment dorsal/ventral axis egg and embryo. To examine how ectopic affects cell fate determination, we constructed an activated version protein. Expression this form (λtop) in follicle cells ovary induces dorsal fates both...
okra ( okr ), spindle-B spnB and spindle-D spnD ) are three members of a group female sterile loci that produce defects in oocyte egg morphology, including variable dorsal–ventral the eggshell embryo, anterior–posterior follicle cell epithelium oocyte, abnormalities nuclear morphology. Many these phenotypes reflect grk-Egfr signaling processes, can be accounted for by failure to accumulate wild-type levels Gurken Fs(1)K10. We have cloned , show encodes Drosophila homolog yeast DNA-repair...
Many signaling proteins and transcription factors that induce pattern organs have been identified, but relatively few of the downstream effectors execute morphogenesis programs. Because such genes may function in many developmental processes, mutations them are expected to be pleiotropic hence ignored or discarded most standard genetic screens. Here we describe a systematic screen designed identify all Drosophila third chromosome (∼40% genome) development tracheal system, tubular respiratory...
Abstract In Drosophila, mutations in double-strand DNA break (DSB) repair enzymes, such as spn-B, activate a meiotic checkpoint leading to dorsal-ventral patterning defects the egg and an abnormal appearance of oocyte nucleus. Mutations spn-D cause array ovarian phenotypes similar spn-B. We have cloned locus found that it encodes protein 271 amino acids shows significant homology human RAD51C protein. mammals spn-B homologs, XRCC3 RAD51C, play role genomic stability somatic cells. To test...
Epithelial tubes that compose many organs are typically long lasting,except under specific developmental and physiological conditions when network remodeling occurs. Although there has been progress elucidating mechanisms of tube formation, little is known the maintain destabilize them during remodeling. Here, we describe Drosophila tendrils mutations compromise maintenance tracheal terminal branches, fine gauge formed by cells ramify on adhere tightly to tissues in order supply with oxygen....
During sprouting angiogenesis in the vertebrate vascular system, and primary branching Drosophila tracheal specialized tip cells direct branch outgrowth network formation. When lumenize, they form subcellular (seamless) tubes. How these seamless tubes are made, shaped maintained remains poorly understood. Here we characterize a mutant called ichor (ich), show that ich is essential for integrity shape of terminal cells. We find Ich regulates tubulogenesis via its role promoting formation...
Tubes are essential for nutrient transport and gas exchange in multicellular eukaryotes, but how connections between different tube types maintained over time is unknown. In the Drosophila tracheal system, mutations oak gall (okg) conjoined (cnj) confer identical defects, including late onset blockage near terminal cell-stalk cell junction ectopic extension of autocellular, seamed tubes into cell. We determined that okg cnj encode E G subunits vacuolar ATPase (vATPase) showed both V0 V1...
Abstract The Germinal Center Kinase III (GckIII) pathway is a Hippo-like kinase module defined by sequential activation of Ste20 kinases Thousand and One (Tao) GckIII, followed nuclear dbf2-related (NDR) Tricornered (Trc). We previously uncovered role for the GckIII in Drosophila melanogaster tracheal (respiratory) tube morphology. trachea form network branched epithelial tubes essential oxygen transport, are structurally analogous to tubular organs vertebrates, such as vascular system. In...
The hexosamine biosynthetic pathway, whose end product is UDP-N acetylglucosamine (UDP-GlcNAc), lies at the base of cellular glycosylation pathways, including lipids, formation heparin sulfated proteoglycans, and N- O-linked proteins. Forward genetic studies in Drosophila have revealed that mutations genes encoding different enzymes pathway result reduction UDP-GlcNAc to extents, with a consequent disruption distinct pathways developmental processes. A maternal zygotic loss-of-function...
Summary Kinase cascades underlie many signaling pathways and are key regulators of development morphogenesis. We have characterized a Hippo-like kinase cascade consisting Thousand One (Tao), Germinal Center III (GckIII/Wheezy), Tricornered (Trc) that plays an essential role in morphogenesis tracheal terminal cell tubes Drosophila . In this cascade, GckIII is the central thought to act together with its binding partner, Cerebral Cavernous Malformations 3 (Ccm3). As suggested by name, Ccm3...
Vessel Plus is an open acccess journal, which publishes articles related to vascular diseases, including acute respiratory distress syndrome, aneurysm, atherosclerosis, hypertension, stroke, peripheral or pulmonary etc.