A5077304115- Retinal Diseases and Treatments
- Connexins and lens biology
- Glaucoma and retinal disorders
- Bee Products Chemical Analysis
- Epigenetics and DNA Methylation
- Moringa oleifera research and applications
- Pharmacological Effects of Natural Compounds
- Natural product bioactivities and synthesis
- Retinal Development and Disorders
- Heme Oxygenase-1 and Carbon Monoxide
- Essential Oils and Antimicrobial Activity
- Neonatal Health and Biochemistry
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cancer-related gene regulation
- Plant Toxicity and Pharmacological Properties
- Ear and Head Tumors
- Phytochemistry and Bioactivity Studies
- Corneal Surgery and Treatments
- Biological and pharmacological studies of plants
- Phytochemical Studies and Bioactivities
- Ocular Infections and Treatments
- Porphyrin Metabolism and Disorders
- Ocular Diseases and Behçet’s Syndrome
- Biochemical effects in animals
- Retinal and Macular Surgery
Indiana University – Purdue University Indianapolis
2021-2025
Indiana University School of Medicine
2021-2025
Indiana University
2023
Hypertension Institute
2022
Bharathidasan University
2015-2021
Abstract Retinal neovascularization impairs visual function and is a hallmark of several neovascular eye diseases, including retinopathy prematurity (ROP) proliferative diabetic (PDR). Current treatments include intravitreal injections anti‐vascular endothelial growth factor (VEGF) biologics, but these therapeutics are often accompanied by high treatment burden resistance to therapy. Prior studies indicate that APE1/Ref‐1, multifunctional protein with both endonuclease (APE1) redox‐mediated...
Abstract Ocular neovascular diseases including age-related macular degeneration (nvAMD) are widespread causes of blindness. Patients’ non-responsiveness to currently used biologics that target vascular endothelial growth factor (VEGF) poses an unmet need for novel therapies. Here, we identify protein arginine methyltransferase 5 (PRMT5) as a therapeutic nvAMD. PRMT5 is well-known epigenetic enzyme. We previously showed methylates and activates proangiogenic proinflammatory transcription...
Objective: An extract (Methanolic) of the Ocimum basilicum leaf was analysed for potential to abrogate experimental formation cataract in-vitro. Methods: Phytoconstituents were first detected in O. extracts (Aqueous or methanolic) by gas chromatographic-mass spectrometric analysis. The putative antioxidant activity these then assessed measuring in-vitro radical-scavenging activity, ion-chelating and reducing potency. Potential cytotoxicity on Human lenticular epithelial B3 (HLE-B3) cells...
Objective: To identify phytoconstituents present in an ethanolic extract of the leaves Tabernaemontana divaricata and to evaluate its in-vitro antioxidant potential. Methods: The was subjected gas chromatography- mass spectrometry analysis phytoconstituents, screened for hydroxyl, superoxide 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, reducing power metal-chelating activity as a measure potential activity. Results: GC-MS revealed presence 96 which 17 are reported be...
Abstract Neovascular age‐related macular degeneration (nAMD) is a leading cause of vision loss in older adults. nAMD treated with biologics targeting vascular endothelial growth factor; however, many patients do not respond to the current therapy. Here, small molecule drug, griseofulvin (GRF), used due its inhibitory effect on ferrochelatase, an enzyme important for choroidal neovascularization (CNV). For local and sustained delivery eyes, GRF encapsulated microparticles based...
The roots of Erythrina lysistemon, growing in Egypt, yielded 24 flavonoid compounds, including 17 pterocarpans, two isoflavanones, one flavanone, isoflavans, 2-arylbenzofuran, and an isoflava-3-ene. Nine pterocarpans have not been reported previously (7–9, 11–14, 19, 20), 11 are here for the first time from this species. Structures were established using HRESIMS, NMR, circular dichroism techniques. Selected compounds tested their ability to block growth human retinal endothelial cells...
Purpose: Heme depletion, through inhibition of ferrochelatase (FECH), blocks retinal and choroidal neovascularization. Both pharmacologic FECH a partial loss-of-function Fech mutation (Fechm1Pas) are associated with decreased However, the ocular physiology Fechm1Pas mice under basal conditions has not been characterized. Here, we aimed to characterize phenotype mice. Methods: We monitored vasculature at postnatal day 17, 2 months, 6 months in homozygotes, heterozygotes, their wild-type...
Ocular Neovascularization Therapy In article number 2306479, Timothy W. Corson, Yoon Yeo, and co-workers encapsulated griseofulvin in polymeric microparticles optimized for intravitreal injection local sustained delivery to the eye. The antiangiogenic drug is released over six weeks block choroidal neovascularization, potential therapy of neovascular age-related macular degeneration.
Laser-induced choroidal neovascularization (L-CNV) in murine models is a standard method for assessing therapies, genetics, and mechanisms relevant to the blinding eye disease neovascular or "wet" age-related macular degeneration. The ex vivo evaluation of these lesions involves confocal microscopy analysis. In via optical coherence tomography (OCT) has previously been established allows longitudinal assessment lesion development. However, produce robust data, many may be required, which can...
Dracaeconolide B (1), a naturally occurring homoisoflavane, was isolated from the red resin of Dracaena cochinchinensis. Efforts have been made to elucidate exact structure compound 1 since it confirmed that dracaeconolide did not contain 7-hydroxy-5,8-dimethoxy moiety. The revised by synthesis three homoisoflavanes containing 5,6,7-trioxygenated moiety each and analysis NMR spectroscopy. proposed as 3-(4-hydroxybenzyl)-7-hydroxy-5,6-dimethoxychromane. Noyori's Ru-catalyzed asymmetric...
Purpose: To evaluate whether a methanolic extract of Ocimum basilicum (OB) leaves prevented lenticular protein alterations in an in-vitro model selenite-induced cataractogenesis.Materials and Methods: Transparent lenses extirpated from Wistar rats were divided into three groups: control; selenite only; treated. Control cultured Dulbecco's modified Eagle's medium (DMEM) alone, only DMEM containing sodium (100 µM selenite/ml DMEM) treated the OB (200 µg extract/ml DMEM); all for 24 h then...
Abstract Ocular neovascular diseases including age-related macular degeneration (nvAMD) are widespread causes of blindness. Patients’ non-responsiveness to currently used biologics that target vascular endothelial growth factor (VEGF) poses an unmet need for novel therapies. Here, we identify protein arginine methyltransferase 5 (PRMT5) as a therapeutic nvAMD. PRMT5 is well-known epigenetic enzyme. We previously showed methylates and activates proangiogenic proinflammatory transcription...