A5054609316- Lung Cancer Treatments and Mutations
- Lung Cancer Research Studies
- RNA modifications and cancer
- Lung Cancer Diagnosis and Treatment
- Nuclear Structure and Function
- Inflammatory mediators and NSAID effects
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Synthesis of β-Lactam Compounds
- DNA Repair Mechanisms
- Cellular Mechanics and Interactions
- Estrogen and related hormone effects
- Synthesis and Biological Activity
- Epigenetics and DNA Methylation
- Protein Structure and Dynamics
- Enzyme Structure and Function
- Synthesis and biological activity
- Hippo pathway signaling and YAP/TAZ
- Wnt/β-catenin signaling in development and cancer
- Renal and related cancers
- Metalloenzymes and iron-sulfur proteins
- PARP inhibition in cancer therapy
- Genomics and Chromatin Dynamics
- Chemical Reactions and Isotopes
- Ion channel regulation and function
Chungbuk National University
2011-2024
Washington State University
2004
Korea Research Institute of Bioscience and Biotechnology
2002-2003
Korea Research Institute of Chemical Technology
1993
Calsequestrin, the major calcium storage protein of both cardiac and skeletal muscle, binds releases large numbers Ca2+ ions for each contraction relaxation cycle. Here we show that two crystal structures calsequestrin are nearly superimposable not only their subunits but also front-to-front-type dimers. binding curves were measured using atomic absorption spectroscopy. This method enables highly accurate measurements even bound to polymerized protein. The complex, with increases correlated...
Abstract The cellular decision regarding whether to undergo proliferation or death is made at the restriction (R)-point, which disrupted in nearly all tumors. identity of molecular mechanisms that govern R-point one fundamental issues cell biology. We found early after mitogenic stimulation, RUNX3 binds its target loci, where it opens chromatin structure by sequential recruitment Trithorax group proteins and cell-cycle regulators drive cells R-point. Soon after, closes these loci recruiting...
Abstract Purpose: RUNX3 is a tumor suppressor gene, which inactivated in approximately 70% of lung adenocarcinomas. Nicotinamide, sirtuin inhibitor, has demonstrated potential re-activating epigenetically silenced cancer cells. This study assessed the therapeutic benefits combining nicotinamide with first-generation EGFR–tyrosine kinase inhibitors (TKI) for patients stage IV carrying EGFR mutations. Patients and Methods: We impact on carcinogen-induced adenocarcinomas mice observed that...
Ca<sup>2+</sup> regulation is coupled to critical signals in eucaryotic cells, and calsequestrin one of the crucial components for this calcium regulation. Our previous observations calsequestrins revealed existence three thioredoxin-like folds, a basic motif that often provides platform small molecule binding. Therefore, we have examined previously reported trifluoperazine other pharmaceuticals similar heart-related side effects (such as tachycardia; bradycardia; palpitation; changing PR,...
Snail contributes to the epithelial-mesenchymal transition by suppressing E-cadherin in transcription processes. The C2H2-type zinc-finger (ZF) domain functions both as a nuclear localization signal which binds importin β directly and DNA-binding domain. Here, 2.5 Å resolution structure of four ZF domains Snail1 complexed with is presented. X-ray reveals that ZFs are required for tight binding import Snail1. shape reminiscent round snail, where ZF1 represents head, ZF2-ZF4 shell, showing...
Polo-like kinase 1 (PLK1), which is crucial in cell cycle regulation, considered a promising anticancer drug target. Herein, we present the N-degron pathway-based proteolysis targeting chimera (PROTAC) for PLK1 degradation, Polo-box domain (PBD). We identified DD-2 as most potent PROTAC that selectively induces degradation cancer cells, including HeLa and nonsmall lung (NSCLC), through pathway. exhibited significant vitro effects, inducing G2/M arrest apoptosis NSCLC lines. showed tumor...
Hikeshi is a nuclear transport receptor required for cell survival after stress. It mediates heat-shock-induced import of 70 kDa heat-shock proteins (Hsp70s) through interactions with FG-nucleoporins (FG-Nups), which are in pore complexes (NPCs). Here, the crystal structure human presented at 1.8 Å resolution. forms an asymmetric homodimer that responsible interaction Hsp70s. The asymmetry arises from distinct conformation C-terminal domain (CTD) and flexibility linker regions each monomer....
Abstract Understanding the structural origins of differences in reduction potentials is crucial to understanding how various electron transfer proteins modulate their and they evolve for diverse functional roles. Here, high‐resolution structures several Clostridium pasteurianum rubredoxin (Cp Rd) variants with changes vicinity redox site are reported order increase this understanding. Our crystal [V44L] (at 1.8 Å resolution), [V44A] (1.6 Å), [V44G] (2.0 Å) [V44A, G45P] (1.5 Rd (all oxidized...
Coronavirus disease 2019 (COVID-19), caused by a new strain of coronavirus called severe acute respiratory syndrome 2 (SARS-CoV-2), is spreading rapidly worldwide. Nafamostat mesylate (NFM) suppresses transmembrane serine protease and SARS-CoV-2 S protein-mediated fusion. In this study, pharmacokinetics lung distribution NFM, administered via intravenous intratracheal routes, were determined using high performance liquid chromatography analysis blood plasma, lumen bronchoalveolar lavage...
The scaffolding protein Salvador (Sav) plays a key role in the Hippo (Hpo) signalling pathway, which controls tissue growth by inhibiting cell proliferation and promoting apoptosis. Dysregulation of pathway contributes to cancer development. Since identification first Sav gene 2002, very little is known regarding molecular basis Sav-SARAH mediating interactions due its insolubility. In this study, refolding (known as WW45)-SARAH provided insight into biochemical biophysical properties,...
Abstract Background: RUNX3 is a tumor suppressor gene, which inactivated in approximately 70% of lung adenocarcinomas. Nicotinamide, sirtuin inhibitor, has demonstrated potential re-activating epigenetically silenced cancer cells. This study assessed the therapeutic benefits combining nicotinamide with first-generation EGFR-TKIs for patients stage IV carrying EGFR mutations. Patients and Methods: We impact on carcinogen-induced adenocarcinomas mice observed that increased levels inhibited...
<p>Figure S4. Serial quality of life (QoL) assessments in nicotinamide vs. placebo groups over 2 years</p>
<p>Figure S1. Effect of Nicotinamide on the expressions RUNX1 and RUNX3 in urethaneinduced mouse lung cancers human cancer cell lines</p>
<p>Figure S4. Serial quality of life (QoL) assessments in nicotinamide vs. placebo groups over 2 years</p>
<p>Figure S3. Spider plot showing individual patient responses over time in both the nicotinamide and placebo groups.</p>
<div>AbstractPurpose:<p><i>RUNX3</i> is a tumor suppressor gene, which inactivated in approximately 70% of lung adenocarcinomas. Nicotinamide, sirtuin inhibitor, has demonstrated potential re-activating epigenetically silenced <i>RUNX3</i> cancer cells. This study assessed the therapeutic benefits combining nicotinamide with first-generation EGFR–tyrosine kinase inhibitors (TKI) for patients stage IV carrying <i>EGFR</i>...