A5033519046- Bacteriophages and microbial interactions
- Microbial infections and disease research
- Bacterial Genetics and Biotechnology
- Vector-borne infectious diseases
- Heat shock proteins research
- Advanced Fluorescence Microscopy Techniques
- Advanced Electron Microscopy Techniques and Applications
- Toxin Mechanisms and Immunotoxins
- RNA and protein synthesis mechanisms
- Toxoplasma gondii Research Studies
- Genomics and Phylogenetic Studies
- Yersinia bacterium, plague, ectoparasites research
- Monoclonal and Polyclonal Antibodies Research
- Cell Image Analysis Techniques
- Bacterial Infections and Vaccines
- Force Microscopy Techniques and Applications
- Streptococcal Infections and Treatments
- RNA Research and Splicing
- Protein Structure and Dynamics
- Viral Infections and Vectors
- ATP Synthase and ATPases Research
- Animal Virus Infections Studies
- Plant Virus Research Studies
- Virus-based gene therapy research
- Aquaculture disease management and microbiota
Institute of Cytology
2014-2024
Bauman Moscow State Technical University
2021
Peter the Great St. Petersburg Polytechnic University
2016-2020
Russian Academy of Sciences
2009-2020
Abstract Numerous studies are devoted to the intestinal microbiota and intercellular communication maintaining homeostasis. In this regard, vesicles secreted by bacteria represent one of most popular topics for research. For example, outer membrane (OMVs) Bacteroides fragilis play an important nutritional role with respect other microorganisms promote anti-inflammatory effects on immune cells. However, toxigenic B. (ETBF) contributes bowel disease, even causing colon cancer. If nontoxigenic...
ABSTRACT During infection, the giant phiKZ phage forms a specialized structure at center of host cell called nucleus. This is crucial for safeguarding viral DNA against bacterial nucleases and segregating transcriptional activities late genes. Here, we describe morphological entity, early infection (EPI) vesicle, which appears to be responsible earlier gene segregation beginning process. Using cryo-electron microscopy, electron tomography (ET), fluorescence microscopy with membrane-specific...
Abstract During infection, the giant phiKZ phage forms a specialized structure at center of host cell called nucleus. This is crucial for safeguarding viral DNA against bacterial nucleases and segregating transcriptional activities late genes. Here, we describe morphological entity, early infection vesicle (EPI vesicle), which appears to be responsible earlier gene segregation beginning process. Using cryo-electron microscopy, electron tomography, fluorescence microscopy with...
A nucleus-like structure composed of phage-encoded proteins and containing replicating viral DNA is formed in Pseudomonas aeruginosa cells infected by jumbo bacteriophage phiKZ. The PhiKZ genes are transcribed independently from host RNA polymerase (RNAP) two RNAPs encoded the phage. virion RNAP (vRNAP) transcribes early must be injected into cell with phage DNA. non-virion (nvRNAP) gene products late genes. In this work, dynamics localization during phiKZ infection were studied. We provide...
The only recognized virulence factor of enterotoxigenic Bacteroides fragilis (ETBF) that accompanies bloodstream infections is the zinc-dependent nonlethal metalloprotease B. toxin (BFT). isolated stimulates intestinal secretion, resulting in epithelial damage and necrosis. Numerous publications have focused on interrelation BFT with inflammation colorectal neoplasia, but nothing known about mechanism its secretion delivery to host cells. However, recent studies gram-negative bacteria shown...
The giant phiKZ phage infection induces the appearance of a pseudo-nucleus inside bacterial cytoplasm. Here, we used RT-PCR, fluorescent in situ hybridization (FISH), electron tomography, and analytical microscopy to study morphology this unique nucleus-like shell demonstrate distribution DNA infected Pseudomonas aeruginosa cells. maturation was traced short intervals for 40 min after revealed continuous spatial separation host DNA. Immediately ejection, located newly-identified round...
Abstract FtsZ – a prokaryotic tubulin homolog is one of the central components bacterial division machinery. At early stage cytokinesis forms so‐called Z‐ring at mid‐cell that guides septum formation. Many approaches were used to resolve structure Z‐ring, however, researchers are still far from consensus on this question. We utilized single‐molecule localization microscopy ( SMLM ) in combination with immunofluorescence staining visualize E sherichia coli fixed cells grown under slow and...
α-Crystallin-type small heat shock proteins (sHsps) are expressed in many bacteria, animals, plants, and archaea. Among mycoplasmas (Mollicutes), predicted sHsp homologues so far were found only the Acholeplasmataceae family. In this report, we describe cloning functional characterization of a novel orthologue, IbpA protein, present Acholeplasma laidlawii. Importantly, similar to endogenously proteins, recombinant protein was able spontaneously generate oligomers vitro rescue chemically...
Small heat shock proteins (sHSPs) are ubiquitous molecular chaperones preventing the irreversible denaturation of proteins. While in Escherichia coli two sHSPs IbpA and IbpB work strong cooperation, sole Mollicute with free-living ability Acholeplasma laidlawii carries a single gene encoding sHSP protein AlIbpA. In vitro, independently temperature, AlIbpA forms heterogeneous mixture approximately 24-mer globules, fibrils huge aggregates. The removal either 12 or 25 N-terminal amino acids led...
Abstract A nucleus-like structure composed of phage-encoded proteins and containing replicating viral DNA is formed in Pseudomonas aeruginosa cells infected by jumbo bacteriophage phiKZ. The PhiKZ genes are transcribed independently from host RNA polymerase (RNAP) two RNAPs encoded the phage. virion RNAP (vRNAP) transcribes early must be injected into cell with phage DNA. non-virion (nvRNAP) products late genes. In this work, dynamics localization during phiKZ infection was studied. We...
Localization microscopy allows visualization of biological structures with resolution well below the diffraction limit. This is achieved by temporal separation single fluorophore molecules emission and subsequent localization them precision few tens nanometers. method was previously successfully used to obtain images FtsZ in Escherichia coli cells using fusion fluorescent protein mEos2. In this work we obtained superresolution fixed E. immunocytochemical labeling. Comparison undergoing...
Serratia proteamaculans synthesizes the intracellular metalloprotease protealysin. This work was aimed at searching for bacterial substrates of protealysin among proteins responsible replication and cell division. We have shown that unlimitedly cleaves SOS response protein RecA. Even 20% cleaved RecA in solution appears to be incorporated into polymer uncleaved monomers, preventing further polymerization inhibiting ATPase activity. Transformation Escherichia coli with a plasmid carrying gene...