A5088860204- Sunflower and Safflower Cultivation
- Metabolomics and Mass Spectrometry Studies
- Bacteriophages and microbial interactions
- Gut microbiota and health
- Bacterial Genetics and Biotechnology
- Clostridium difficile and Clostridium perfringens research
- Phytoplasmas and Hemiptera pathogens
- Fatty Acid Research and Health
- Microbial infections and disease research
- Infant Nutrition and Health
- Bacterial Identification and Susceptibility Testing
- Mass Spectrometry Techniques and Applications
- Studies on Chitinases and Chitosanases
- Genomics and Phylogenetic Studies
- Microscopic Colitis
- Enzyme Structure and Function
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Aquaculture disease management and microbiota
- Lipid metabolism and biosynthesis
- Spectroscopy and Chemometric Analyses
- Microbial Community Ecology and Physiology
- Insect symbiosis and bacterial influences
- Streptococcal Infections and Treatments
- Diet and metabolism studies
- Metabolism and Genetic Disorders
Skolkovo Institute of Science and Technology
2018-2025
Weizmann Institute of Science
2023
All-Russian Research Institute of Oil Crops by V.S. Pustovoit
2020
Vavilov Institute of General Genetics
2020
Southern California University for Professional Studies
2020
University of Southern California
2020
Federal Medical-Biological Agency
2017-2019
Federal Research and Clinical Center of Physical-Chemical Medicine named after Y.M. Lopukhin
2014-2017
Physico-Technical Institute
2014
Kurchatov Institute
2011-2012
Significance Biocompatible microfluidic double water-in-oil-in-water emulsion (MDE) enables in-droplet cultivation of different living species. The combination droplet-generating machinery with FACS followed by next-generation sequencing and liquid chromatography-mass spectrometry analysis the secretomes encapsulated organisms yielded detailed genotype/phenotype descriptions. MDE–FACS platform we developed enabled highly sensitive single-cell selection predesigned activity exploration...
Abstract Numerous studies are devoted to the intestinal microbiota and intercellular communication maintaining homeostasis. In this regard, vesicles secreted by bacteria represent one of most popular topics for research. For example, outer membrane (OMVs) Bacteroides fragilis play an important nutritional role with respect other microorganisms promote anti-inflammatory effects on immune cells. However, toxigenic B. (ETBF) contributes bowel disease, even causing colon cancer. If nontoxigenic...
Microbiome spectra serve as critical clues to elucidate the evolutionary biology pathways, potential pathologies, and even behavioral patterns of host organisms. Furthermore, exotic sources microbiota represent an unexplored niche discover microbial secondary metabolites. However, establishing bacterial functionality is complicated by intricate web interactions inside microbiome. Here we apply ultrahigh-throughput (uHT) microfluidic droplet platform for activity profiling entire oral...
Genes coding for small peptides have been frequently misannotated as long noncoding RNA (lncRNA) genes. Here we demonstrated that one such transcript is translated into a 56-amino-acid-long peptide conserved in chordates, corroborating the work published while this manuscript was under review. The Mtln could be detected mitochondria of mouse cell lines and tissues. In line with its mitochondrial localization, lack decreases activity respiratory chain complex I. Unlike integral components...
Importance No clinically applicable diagnostic test exists for severe mental disorders. Lipids harbor potential as disease markers. Objective To define a reproducible profile of lipid alterations in the blood plasma patients with schizophrenia (SCZ) independent demographic and environmental variables to investigate its specificity association other psychiatric disorders, ie, major depressive disorder (MDD) bipolar (BPD). Design, Setting, Participants This was multicohort case-control...
Abstract Autism spectrum disorder (ASD) is a common neurodevelopmental with yet incompletely uncovered molecular determinants. Alterations in the abundance of low weight compounds (metabolites) ASD could add to our understanding disease. Indeed, such alterations take place urine, plasma and cerebellum individuals. In this work, we investigated mass-spectrometric signal intensities 1,366 metabolites prefrontal cortex grey matter 32 40 control 15% these showed significantly different clustered...
Introduction Bacteroides fragilis (Bf)’s T6SS locus has been characterized and shown to have functional activity in competition experiments. It demonstrated that symbiont nontoxigenic Bf strains a more effective “weapon” effect on pathogenic Bf, which is realized through the of effector-immune (E-I) protein pairs. Intensive study structure led an understanding certain issues related its activity, but exact regulatory mechanisms E-I pair remain unclear. Proteomic annotation components...
Sunflower is an important oilseed crop domesticated in North America approximately 4000 years ago. During the last century, oil content sunflower was under strong selection. Further improvement of properties achieved by modulating its fatty acid composition one main directions modern breeding.We searched for genetic basis variation genotyping 601 inbred lines and assessing their lipid composition. Our genome-wide association analysis based on genotypes 15,483 SNPs concentrations 23 acids,...
The only recognized virulence factor of enterotoxigenic Bacteroides fragilis (ETBF) that accompanies bloodstream infections is the zinc-dependent nonlethal metalloprotease B. toxin (BFT). isolated stimulates intestinal secretion, resulting in epithelial damage and necrosis. Numerous publications have focused on interrelation BFT with inflammation colorectal neoplasia, but nothing known about mechanism its secretion delivery to host cells. However, recent studies gram-negative bacteria shown...
Abstract What strategies do bacteria employ for adaptation to their hosts and are these different varied hosts? To date, many studies on the interaction of bacterium its host have been published. However, global changes in bacterial cell process invasion persistence, remain poorly understood. In this study, we demonstrated phase transition avian pathogen Mycoplasma gallisepticum upon various types eukaryotic cells (human, chicken, mouse) which was stable during several passages after...
Microorganisms and their hosts communicate with each other by secreting numerous components. This cross-kingdom cell-to-cell signaling involves proteins small molecules, such as metabolites. These compounds can be secreted across the membrane via transporters may also packaged in outer vesicles (OMVs). Among components, volatile (VOCs) are of particular interest, including butyrate propionate, which have proven effects on intestinal, immune, stem cells. Besides short fatty acids, groups...
To date, no genome of any the species from genus Spiroplasma has been completely sequenced. Long repetitive sequences similar to mobile units present a major obstacle for current sequencing technologies. Here, we report assembly melliferum KC3 into 4 contigs, followed by proteogenomic annotation and metabolic reconstruction based on discovery 521 expressed proteins comprehensive metabolomic profiling. A systems approach allowed us elucidate putative pathogenicity mechanisms discover...
HU proteins belong to the nucleoid-associated (NAPs) that are involved in vital processes such as DNA compaction and reparation, gene transcription etc. No data available on structures of from mycoplasmas. To this end, protein parasitic mycoplasma Spiroplasma melliferum KC3 was cloned, overexpressed Escherichia coli purified homogeneity. Prismatic crystals were obtained by vapour-diffusion technique at 4°C. The diffracted 1.36 Å resolution (the best ever for a protein). diffraction indexed...
We present a systematic study of three bacterial species that belong to the class Mollicutes, smallest and simplest bacteria, Spiroplasma melliferum, Mycoplasma gallisepticum, Acholeplasma laidlawii. To understand difference in basic principles metabolism regulation adaptation environmental conditions species, we analyzed metabolome these bacteria. Metabolic pathways were reconstructed using proteogenomic annotation data provided by our lab. The results metabolome, proteome genome profiling...
ABSTRACT Inflammatory bowel diseases are characterized by chronic intestinal inflammation and alterations in the gut microbiota composition. Bacteroides fragilis , which secretes outer membrane vesicles (OMVs) with polysaccharide A (PSA), can moderate inflammatory response possibly alter In this study, we created a murine model of sodium dextran sulfate (DSS)-induced colitis treated it B. OMVs. We monitored efficiency OMV therapy determining disease activity index (DAI) performing...
Abstract Background Lipids contained in milk are an essential source of energy and structural materials for a growing neonate. Furthermore, lipids’ long-chain unsaturated fatty acid residues can directly participate neonatal tissue formation. Here, we used untargeted mass spectrometric measurements to assess lipid composition seven mammalian species: humans, two macaque species, cows, goats, yaks, pigs. Results Analysis the main class, triacylglycerides (TAGs), revealed species-specific...
Investigation of archeological and paleontological artifacts at the molecular level has become a trend over recent years. Paleogenomics provides information about evolution ancient species; paleoproteomics was recently established as branch conventional proteomics dedicated to study proteomes extinct organisms. At same time paleolipidomics, which could focus on investigation lipids species, is not developed yet, though fossil may have potential provide past diseases or diet. Here we present...
Abstract Despite the fact term “proteome” was proposed to characterize a set of proteins in one mycoplasma species, proteome response various exposures this bacteria are still obscure. Commonly, authors studying proteomic on perturbation models mycoplasmas use single approach and do not confirm their findings by alternative methods. Consequently, results analysis should be validated complementary techniques. In study we utilized three approaches (SWATH, MRM, 2D-DIGE) assess Mycoplasma...
Oilseed crops are one of the most important sources vegetable oils for food and industry. Nutritional technical properties oil primarily determined by its fatty acid (FA) composition. The content composition FAs in plants commonly using gas chromatography-mass spectrometry (GS-MS) or chromatography-flame ionization detection (GC-FID) techniques. In present work, we applied ultra-performance liquid (UPLC-MS) technique to FA profiling sunflower rapeseed seeds compared this method with GC-FID...
Investigation of archeological and paleontological artifacts at the molecular level has become a trend over recent years. Paleogenomics provides information about evolution ancient speci...
Abstract Inflammatory bowel diseases are characterized by chronic intestinal inflammation and alterations of gut microbiota composition. Bacteroides fragilis , which secretes outer membrane vesicles (OMVs) with polysaccharide A (PSA), can moderate inflammatory response possibly alter In this research, we created a murine model DSS- induced colitis treated it OMVs. We monitored the efficiency OMVs therapy determining disease activity index (DAI) histological examination (HE) intestine before...
Abstract The gut is under complex attack by a large number of biologically active molecules, including bacterial enzymes, metabolites, nucleic acids and immuno-active compounds. Most these components are presented in outer membrane vesicles (OMVs), which actively produced all types. Bacteroides fragilis , as member the microbiota, has unique OMV’s that known to have both pathogenic positive immunomodulatory properties. OMVs well characterized proteomic metabolomic methods therefore represent...