A5010578396- Bacteriophages and microbial interactions
- Microbial infections and disease research
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- Blood groups and transfusion
- Aquaculture disease management and microbiota
- Plant and Fungal Interactions Research
- Yersinia bacterium, plague, ectoparasites research
- Reproductive tract infections research
- Bacterial Identification and Susceptibility Testing
- Advanced biosensing and bioanalysis techniques
- Mycorrhizal Fungi and Plant Interactions
- Vibrio bacteria research studies
- Plant Virus Research Studies
- Plant Parasitism and Resistance
- Yeasts and Rust Fungi Studies
- Antibiotic Resistance in Bacteria
- Plant Reproductive Biology
- Salmonella and Campylobacter epidemiology
- Plant Pathogens and Fungal Diseases
- Plant Molecular Biology Research
- Escherichia coli research studies
- Phytoplasmas and Hemiptera pathogens
- Viral Infections and Vectors
InSysBio (Russia)
2022-2025
Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing
2025
Institute for Systems Biology
2024
Federal Medical-Biological Agency
2019-2022
Federal Research and Clinical Center of Physical-Chemical Medicine named after Y.M. Lopukhin
2015-2022
Lomonosov Moscow State University
2016-2017
Introduction Bacteroides fragilis (Bf)’s T6SS locus has been characterized and shown to have functional activity in competition experiments. It demonstrated that symbiont nontoxigenic Bf strains a more effective “weapon” effect on pathogenic Bf, which is realized through the of effector-immune (E-I) protein pairs. Intensive study structure led an understanding certain issues related its activity, but exact regulatory mechanisms E-I pair remain unclear. Proteomic annotation components...
Mycoplasma pneumoniae (MP) is a common bacterial respiratory infection that can cause pneumonia, particularly in children. Previously published data have highlighted the high incidence of viral co-infections and problem increasing macrolide resistance MP worldwide. (1) to estimate impact infections circulating local population on spectrum co-infection hospitalized children with pneumonia (MPP), (2) determine if there are differences mutation rate for samples from hospitals Russia located...
DNA repair is essential for the maintenance of genome stability in all living beings. Genome size as well repertoire and abundance components may vary among prokaryotic species. The bacteria Mollicutes class feature a small size, absence cell wall, parasitic lifestyle. A number genes make good model "minimal cell" concept.In this work we studied system Mycoplasma gallisepticum on genomic, transcriptional, proteomic levels. We detected 18 out 22 members protein level. found that respective...
Abstract What strategies do bacteria employ for adaptation to their hosts and are these different varied hosts? To date, many studies on the interaction of bacterium its host have been published. However, global changes in bacterial cell process invasion persistence, remain poorly understood. In this study, we demonstrated phase transition avian pathogen Mycoplasma gallisepticum upon various types eukaryotic cells (human, chicken, mouse) which was stable during several passages after...
Mollicutes (mycoplasmas) feature a significant loss of known regulators gene expression. Here, we identified the recognition site MraZ-family regulator Mycoplasma gallisepticum, which is conserved in many species different clades within class Mollicutes. The MraZ binding AAAGTG[T/G], promoter mraZ it forms series direct repeats with structure (AAAGTG[T/G]N3)k, where k = 3 most frequently. binds to single repeat as an octamer complex. can also bind or spacer lengths (2-4 nt); thus, may play...
Crohn's disease (CD) is a severe chronic immune-mediated granulomatous inflammatory of the gastrointestinal tract. The mechanisms CD pathogenesis remain obscure. Metagenomic analysis samples from patients revealed that several them have elevated level
Bacteria of the class Mollicutes have significantly reduced genomes and gene expression control systems. They are also efficient pathogens that can colonize a broad range hosts including plants animals. Despite their simplicity, demonstrate complex transcriptional responses to various conditions, which contradicts reduction in regulation mechanisms. We analyzed conservation distribution transcription regulators across 50 species. The majority factors regulate transport metabolism, there four...
Introduction.Mycoplasma hominis is a bacterium belonging to the class Mollicutes. It causes acute and chronic infections of urogenital tract. The main features this are an absence cell wall reduced genome size (517-622 protein-encoding genes). Previously, we have isolated morphologically unknown M. colonies called micro-colonies (MCs) from serum patients with inflammatory tract infection.Hypothesis. MCs functionally different typical (TCs) in terms metabolism division.Aim. To determine...
At present, bacteriophages are considered as an alternative to antibiotics in prevention and treatment of bacterial infections, particular cholera. The aim the work was demonstrate a method obtain synthetic bacteriophage against Vibrio cholerae . Vibriophage N4 selected subject for study. Materials methods genome sequence vibriophage (38.5 kb) taken from NCBI GenBank database. divided into gene blocks 1500–2000 bp. blocks, turn, were split oligonucleotides. Sequence partitioning carried out...
The standardization of DNA fragment assembly methods for many tasks synthetic biology is crucial. This necessary synthesizing a wider repertoire sequences, as well further automation and miniaturization such reactions. In this work, we proposed conditions the fragments from chemically synthesized oligonucleotides identified errors occurring in sequence under these conditions. Additionally, combining into larger fragments. We showed that optimized are suitable wide range sequences.
Introduction WhiA is a conserved protein found in numerous bacteria. It consists of an HTH DNA-binding domain linked with homing endonuclease (HEN) domain. one the most transcription factors reduced bacteria class Mollicutes. Its function Mollicutes unknown, while it well-characterized Streptomyces . Here, we focused on from Mycoplasma gallisepticum Methods We used combination molecular dynamics, EMSA, MST and AFM to study ATP-binding properties M. The transcriptional repressor was...
Mycoplasma hominis is an opportunistic urogenital pathogen in vertebrates. It a non-glycolytic species that produces energy via arginine degradation. Among genital mycoplasmas, M. the most commonly reported to play role systemic infections and can persist host for long time. However, it unclear how proceeds under limitation. The recent metabolic reconstruction of has demonstrated its ability catabolize deoxyribose phosphate produce ATP. In this study, we cultivated on two different sources...
The epigenetics of bacteria, and bacteria with a reduced genome in particular, is great interest, but still poorly understood. Mycoplasma gallisepticum, representative the class Mollicutes, an excellent model minimal cell because its size, lack wall, primitive organization. In this study we investigated DNA modifications object gallisepticum their roles. We identified methylation motifs M. S6 at level using single molecule real time (SMRT) sequencing. Only ANCNNNNCCT motif was found genome....
Bacteria of class Mollicutes (mycoplasmas) feature significant genome reduction which makes them good model organisms for systems biology studies. Previously we demonstrated, that drastic transcriptional response mycoplasmas to stress results in a very limited on the level protein. In this study used heat M. gallisepticum and ribosome profiling elucidate process genetic information transfer under stress. We found ribosomes demonstrate selectivity towards mRNA binding. identified may be...
Mycoplasmas are pathogenic, genome-reduced bacteria. The development of such fields science as system and synthetic biology is closely associated with them. Despite intensive research different representatives this genus, genetic manipulations remain challenging in mycoplasmas. Here we demonstrate a single-plasmid transposon-based CRISPRi for the repression gene expression We show that selected determinants provide level dCas9 does not lead to significant slow-down mycoplasma growth. For...
The development of high-throughput technologies is increasingly resulting in identification numerous cases low correlation between mRNA and the protein level cells. These controversial observations were made on various bacteria, such as E. coli, Desulfovibrio vulgaris, Lactococcus lactis. Thus, it important to develop technologies, including techniques, aimed at studying gene expression regulation translation. In current study, we performed proteomic profiling M. gallisepticum ribosomes...
Bacteria of the class Mollicutes underwent extreme reduction genomes and gene expression control systems. Only a few regulators are known to date. In this work, we describe novel group transcriptional that distributed within different restriction-modification systems (RM-systems).We performed cross-species search putative RM-systems (C-proteins) respective binding sites in Mollicutes. We identified set C-protein motifs studied most frequent motif on model Mycoplasma gallisepticum S6....
Mycoplasma hominis is an opportunistic bacterium that can cause acute and chronic infections of the urogenital tract. This bacterium, like all other species, characterized by reduced genome size, and, consequently, reduction main metabolic pathways. M. cells cannot effectively use glucose as a carbon energy source. Therefore, pathway metabolism arginine dihydrolase pathway. However, several bacteria nucleosides sole Biochemical studies using Salmonella typhimurium have shown three enzymes...
WhiA is a conserved protein that widespread in several groups of bacteria. Its function significantly differs across bacterial taxons and poorly understood except for its activity sporulation control Streptomyces. We demonstrated mycoplasmas (Mollicutes) transcriptional regulator the ribosomal proteins operon an ATP sensor. Knockdown whiA gene results significant growth retardation which indicates importance.
The structure and dynamics of bacterial nucleoids play important roles in regulating gene expression. Bacteria class Mollicutes and, particular, mycoplasmas feature extremely reduced genomes. They lack multiple structural proteins the nucleoid, as well regulators We studied organization Mycoplasma gallisepticum stationary exponential growth phases at protein levels. phase transition results reorganization M. nucleoid. In it undergoes condensation changes content. observed corroborate with...
Mycoplasma gallisepticum is a bacterium of class Mollicutes which encompasses wall-less bacteria with significantly reduced genomes. Due to their overall reduction and simplicity mycoplasmas serve as model minimal cell are used for systems biology studies. Here we present raw data on translatome (ribosome-bound mRNA) analysis under logarithm growth heat stress. The supports the publication "Ribosomal profiling gallisepticum" (G. Y. Fisunov, D. V Evsyutina, A. Arzamasov, I. O. Butenko, V. M....