A5019809932- Microbial infections and disease research
- Bacteriophages and microbial interactions
- Aquaculture disease management and microbiota
- SARS-CoV-2 and COVID-19 Research
- vaccines and immunoinformatics approaches
- Bacterial Identification and Susceptibility Testing
- Viral Infections and Vectors
- Clostridium difficile and Clostridium perfringens research
- Metal Extraction and Bioleaching
- Gut microbiota and health
- Blood groups and transfusion
- Leech Biology and Applications
- Bacterial Infections and Vaccines
- Immunotherapy and Immune Responses
- COVID-19 Clinical Research Studies
- Effects and risks of endocrine disrupting chemicals
- Long-Term Effects of COVID-19
- Microscopic Colitis
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Venomous Animal Envenomation and Studies
- Neurological Disorders and Treatments
- Heparin-Induced Thrombocytopenia and Thrombosis
- Healthcare and Venom Research
- Arsenic contamination and mitigation
- Mine drainage and remediation techniques
InSysBio (Russia)
2022-2025
Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing
2025
Federal Medical-Biological Agency
2017-2023
IFC Research (United Kingdom)
2023
Federal Research and Clinical Center of Physical-Chemical Medicine named after Y.M. Lopukhin
2016-2022
Abstract Background Salivary cell secretion (SCS) plays a critical role in blood feeding by medicinal leeches, making them of use for certain medical purposes even today. Results We annotated the Hirudo medicinalis genome and performed RNA-seq on salivary cells isolated from three closely related leech species, H. medicinalis, orientalis , verbana . Differential expression analysis verified proteomics identified cell-specific gene expression, many which encode previously unknown components....
The COVID-19 pandemic was the most dramatic in newest history with nearly 7 million deaths and global impact on mankind. Here, we report binding index of 305 human leukocyte antigen (HLA) class I molecules from 18,771 unique haplotypes 28,104 individuals to 821 peptides experimentally observed spike protein receptor domain (RBD) five main SARS-CoV-2 strains hydrolyzed by proteasomes constitutive immune catalytic phenotypes. Our data read that mutations angiotensin-converting enzyme 2...
Mycoplasma pneumoniae (MP) is a common bacterial respiratory infection that can cause pneumonia, particularly in children. Previously published data have highlighted the high incidence of viral co-infections and problem increasing macrolide resistance MP worldwide. (1) to estimate impact infections circulating local population on spectrum co-infection hospitalized children with pneumonia (MPP), (2) determine if there are differences mutation rate for samples from hospitals Russia located...
COVID-19 caused by SARS-CoV-2 is continuing to spread around the world and drastically affect our daily life. New strains appear, severity of course disease itself seems be decreasing, but even people who have been ill on an outpatient basis suffer post-COVID consequences. Partly, it associated with autoimmune reactions, so debates about development new vaccines need for vaccination/revaccination continue. In this study we performed analysis antibody response patients linear conformational...
Abstract What strategies do bacteria employ for adaptation to their hosts and are these different varied hosts? To date, many studies on the interaction of bacterium its host have been published. However, global changes in bacterial cell process invasion persistence, remain poorly understood. In this study, we demonstrated phase transition avian pathogen Mycoplasma gallisepticum upon various types eukaryotic cells (human, chicken, mouse) which was stable during several passages after...
The commensal microflora provides a repertoire of antigens that illicit mucosal antibodies. In some cases, these antibodies can cross-react with host proteins, inducing autoimmunity, or other microbial antigens. We demonstrate the oral microbiota induce salivary anti-SARS-CoV-2 Spike IgG via molecular mimicry. Anti-Spike in saliva correlated enhanced abundance Streptococcus salivarius 1 month after vaccination. Several human bacteria, including S. salivarius, were recognized by...
Crohn's disease (CD) is a severe chronic immune-mediated granulomatous inflammatory of the gastrointestinal tract. The mechanisms CD pathogenesis remain obscure. Metagenomic analysis samples from patients revealed that several them have elevated level
Biooxidation of gold-bearing arsenopyrite concentrates, using acidophilic microbial communities, is among the largest commercial biohydrometallurgical processes. However, molecular mechanisms responses to sulfide raw materials have not been widely studied. The goal this research was gain insight into defense strategies bacterium Sulfobacillus thermotolerans, which dominates communities functioning in industrial biooxidation processes at >35 °C, against toxic effect high-arsenic...
Introduction.Mycoplasma hominis is a bacterium belonging to the class Mollicutes. It causes acute and chronic infections of urogenital tract. The main features this are an absence cell wall reduced genome size (517-622 protein-encoding genes). Previously, we have isolated morphologically unknown M. colonies called micro-colonies (MCs) from serum patients with inflammatory tract infection.Hypothesis. MCs functionally different typical (TCs) in terms metabolism division.Aim. To determine...
Mutations in surface proteins enable emerging variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) to escape a substantial fraction neutralizing antibodies and may thus weaken vaccine-driven immunity. To compare available vaccines justify revaccination, rapid evaluation antibody (Ab) responses currently circulating SARS-CoV-2 interest (VOI) concern (VOC) is needed. Here, we developed multiplex protein microarray-based system for profiling anti-SARS-CoV-2 Ab levels human...
The epigenetics of bacteria, and bacteria with a reduced genome in particular, is great interest, but still poorly understood. Mycoplasma gallisepticum, representative the class Mollicutes, an excellent model minimal cell because its size, lack wall, primitive organization. In this study we investigated DNA modifications object gallisepticum their roles. We identified methylation motifs M. S6 at level using single molecule real time (SMRT) sequencing. Only ANCNNNNCCT motif was found genome....
The SARS-CoV-2 pandemic is a big challenge for humanity. COVID-19 severity differs significantly from patient to patient, and it important study the factors protecting severe forms of disease. Respiratory microbiota may influence patient's susceptibility infection disease due its ability modulate immune system response host organism. This data article describes microbiome dataset upper respiratory tract positive patients Russia. reports microbial community profile 335 human nasopharyngeal...
Abstract Despite the fact term “proteome” was proposed to characterize a set of proteins in one mycoplasma species, proteome response various exposures this bacteria are still obscure. Commonly, authors studying proteomic on perturbation models mycoplasmas use single approach and do not confirm their findings by alternative methods. Consequently, results analysis should be validated complementary techniques. In study we utilized three approaches (SWATH, MRM, 2D-DIGE) assess Mycoplasma...
Abstract The commensal microflora is a source for multiple antigens that may induce cross-reactive antibodies against host proteins and pathogens. However, whether bacteria can SARS-CoV-2 remains unknown. Here we report several contribute to the generation of IgA receptor-binding domain (RBD) Spike protein. We identified unexposed individuals with RBD-binding at their mucosal surfaces. Conversely, neutralising monoclonal anti-RBD recognised distinct bacterial species. Some these bacteria,...
Abstract The microbiota of the respiratory tract remains a relatively poorly studied subject. At same time, like intestinal microbiota, it is involved in modulating immune response to infectious agents host organism. A causal relationship between composition and likelihood development severity COVID-19 may be hypothesized. We analyze biomaterial from nasopharyngeal smears 336 patients with confirmed diagnosis COVID-19, selected during first second waves epidemic Russia. Sequences similar...
Abstract The gut is under complex attack by a large number of biologically active molecules, including bacterial enzymes, metabolites, nucleic acids and immuno-active compounds. Most these components are presented in outer membrane vesicles (OMVs), which actively produced all types. Bacteroides fragilis , as member the microbiota, has unique OMV’s that known to have both pathogenic positive immunomodulatory properties. OMVs well characterized proteomic metabolomic methods therefore represent...
SUMMARY The COVID-19 pandemic was the most dramatic in newest history with nearly 7 million deaths and global impact on mankind. Here we report binding index of 305 HLA class I molecules from 18,771 unique haplotypes 28,104 individuals to 821 peptides experimentally observed spike protein RBD 5 main SARS-CoV-2 strains hydrolyzed by human proteasomes constitutive immune catalytic phenotypes. Our data read that mutations hACE2-binding region 496-513 Omicron B.1.1.529 strain results a increase...
Agricultural and industrial activities are increasing pollution of water bodies with low doses xenobiotics that have detrimental effects on aquaculture. The aim this work was to determine the possibility using Levilactobacillus brevis 47f culture in fish aquaculture under influence as an adaptogen. An increase survival Danio rerio individuals exposed xenobiotic bisphenol A solution fed L. shown compared control groups and, at same time, cytokine profile intestinal tissues also investigated....
The data reported is a large-scale untargeted proteome profile for Mycoplasma gallisepticum - model organism studying both regulation in genome-reduced bacteria and intracellular infection (Mazin et al., 2014) [1,2]. While seminal whole-proteome studies were performed on genitalium [3] few datasets are available pneumoniae, no data-independent (DIA) profiling has been published of genus. Since DIA-based allows to extract evidence presence quantity any protein interest post-acquisition manner...
The genus Mycoplasma relates to Gram-positive bacteria that lack a cell wall and are capable cause chronic disease in humans animals. Among the agents of infection domestic poultry wild birds, gallisepticum is most important mycoplasma species, causing considerable losses industry. In present paper, we provide data on adaptation M. eukaryotic host cells genomic level. major changes were predominantly localized VlhA-hemagglutinin genes which components pathogenesis. ability mycoplasmas change...