A5076781905- Lipid Membrane Structure and Behavior
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- RNA and protein synthesis mechanisms
- Protein Structure and Dynamics
- Regional Economic Development and Innovation
- Arctic and Russian Policy Studies
- Economic and Technological Developments in Russia
- Synthesis and characterization of novel inorganic/organometallic compounds
- Regional Socio-Economic Development Trends
- Growth Hormone and Insulin-like Growth Factors
- Ferrocene Chemistry and Applications
- Receptor Mechanisms and Signaling
- Synthetic Organic Chemistry Methods
- Alzheimer's disease research and treatments
- Computational Drug Discovery Methods
- Fluorine in Organic Chemistry
- Ion channel regulation and function
- Digitalization and Economic Development in Agriculture
- ATP Synthase and ATPases Research
- Digital Economy and Transformation
- RNA Interference and Gene Delivery
- Water Resources and Management
- Oxidative Organic Chemistry Reactions
- Neuroscience and Neuropharmacology Research
Federal Research and Clinical Center of Physical-Chemical Medicine named after Y.M. Lopukhin
2017-2023
Institute of Bioorganic Chemistry
2009-2023
Euphrosyne Polotskaya State University
2022
Plekhanov Russian University of Economics
2020-2021
Center for Theoretical Problems of Physicochemical Pharmacology
2021
Russian University of Cooperation
2021
V. N. Karazin Kharkiv National University
2020
Frumkin Institute of Physical Chemistry and Electrochemistry
1994-2017
Kamsky Institute of Humanitarian and Engineering Technologies
2016
Izhevsk State Agricultural Academy
2012
Lipid membranes serve as effective barriers allowing cells to maintain internal composition differing from that of extracellular medium. Membrane permeation, both natural and artificial, can take place via appearance transversal pores. The rearrangements lipids leading pore formation in the intact membrane are not yet understood details. We applied continuum elasticity theory obtain continuous trajectory closure, analyzed molecular dynamics trajectories pre-formed reseal. hypothesized a is...
Abstract Lipid membranes are extremely stable envelopes allowing cells to survive in various environments and maintain desired internal composition. Membrane permeation through formation of transversal pores requires substantial external stress. Practically, usually formed by application lateral tension or transmembrane voltage. Using the same approach as was used for obtaining continuous trajectory pore stress-less membrane previous article, we now consider process under The waiting time...
Alzheimer's disease (AD) pathogenesis is correlated with the membrane content of various lipid species, including cholesterol, whose interactions amyloid precursor protein (APP) have been extensively explored. Amyloid-β peptides triggering AD are products APP cleavage by secretases, which differ depending on and secretase location relative to ordered or disordered microdomains. We used high-resolution NMR probe cholesterol analog transmembrane domain in two membrane-mimicking systems...
BNip3 is a prominent representative of apoptotic Bcl-2 proteins with rather unique properties initiating an atypical programmed cell death pathway resembling both necrosis and apoptosis. Many family modulate the permeability state outer mitochondrial membrane by forming homo- hetero-oligomers. The structure dynamics homodimeric transmembrane domain were investigated aid solution NMR in lipid bicelles molecular energy relaxation explicit bilayer. right-handed parallel helix-helix hydrogen...
Based on the 1H-15N NMR spectroscopy data, three-dimensional structure and internal dynamic properties of ribosomal protein L7 from Escherichia coli were derived. The dimer in solution can be described as a set three distinct domains, tumbling rather independently linked via flexible hinge regions. dimeric N-terminal domain (residues 1-32) consists two antiparallel α-α-hairpins forming symmetrical four-helical bundle, whereas identical C-terminal domains 52-120) adopt compact α/β-fold. There...
The epidermal growth factor receptor (EGFR) family is an important class of tyrosine kinases, mediating a variety cellular responses in normal biological processes and pathological states multicellular organisms. Different modes dimerization the human EGFR transmembrane domain (TMD) different membrane mimetics recently prompted us to propose novel signal transduction mechanism based on protein-lipid interaction. However, experimental evidence for it was originally obtained with slightly TMD...
COVID-19 caused by SARS-CoV-2 is continuing to spread around the world and drastically affect our daily life. New strains appear, severity of course disease itself seems be decreasing, but even people who have been ill on an outpatient basis suffer post-COVID consequences. Partly, it associated with autoimmune reactions, so debates about development new vaccines need for vaccination/revaccination continue. In this study we performed analysis antibody response patients linear conformational...
7-Dehydrocholesterol, an immediate metabolic predecessor of cholesterol, can accumulate in tissues due to some abnormalities, causing array symptoms known as Smith–Lemli–Opitz syndrome. Enrichment cellular membranes with 7-dehydrocholesterol interferes normal cell-signaling processes, which involve interaction between rafts and formation the so-called signaling platforms. In model membranes, cholesterol-based ordered domains usually merge upon contact. According our experimental data,...
Sphingomyelin- and cholesterol- enriched membrane domains, commonly referred to as “rafts” play a crucial role in large number of intra- intercellular processes. Recent experiments suggest that not only the volumetric inhomogeneity lipid distribution rafts, but also arrangement 1D boundary between raft surrounding is important for membrane-associated The reason preferentially recruits different peptides, such HIV (human immunodeficiency virus) fusion peptide. In present work, we report...
Membrane fusion mediates multiple vital processes in cell life. Specialized proteins mediate the process, and a substantial part of their energy is used for topological rearrangement membrane lipid matrix. Therefore, elastic parameters bilayers are crucial importance determination barriers that have to be crossed process take place. In case enveloped viruses (e.g., influenza) with endosomal membrane, interacting membranes an acidic environment, which can affect membrane’s mechanical...
Fusion of cellular membranes during normal biological processes, including proliferation, or synaptic transmission, is mediated and controlled by sophisticated protein machinery ensuring the preservation vital barrier function membrane throughout process. virus particles with host cell more sparingly arranged often a single fusion protein, can afford to be less discriminative towards possible different outcomes attempts. Formation leaky intermediates was recently observed in some an...
Alzheimer's disease is an age-related pathology associated with accumulation of amyloid-β peptides, products enzymatic cleavage precursor protein (APP) by secretases. Several familial mutations causing early onset the have been identified in APP transmembrane (TM) domain. The influence production amyloid-β, but molecular mechanisms this effect are unclear. "Australian" (L723P) mutation located C-termini TM domain autosomal-dominant, disease. Herein, we describe impact L723P on...
Human InsR, IGF1R, and IRR receptor tyrosine kinases (RTK) of the insulin subfamily play an important role in signaling pathways for a wide range physiological processes are directly associated with many pathologies, including neurodegenerative diseases. The disulfide-linked dimeric structure these receptors is unique among RTKs. Sharing high sequence homology, differ dramatically their localization, expression, functions. In this work, using high-resolution NMR spectroscopy supported by...
In 1-adamantyl-2,8,9-trioxa-5-aza-1-germabicyclo[3.3.3]undecane or 1-adamantylgermatrane, [Ge(C(10)H(15))(C(6)H(12)NO(3))], (I), and (2,8,9-trioxa-5-aza-1-germabicyclo[3.3.3]undecan-1-yl)methyl N-cyclohexylcarbamate [(germatran-1-yl)methyl] N-cyclohexylcarbamate, [Ge(C(6)H(12)NO(3))(C(8)H(14)NO(2))], (II), the Ge atoms are characterized by trigonal-bypiramidal configurations. The Ge...N distances [2.266 (3) 2.206 A in (I) respectively] among longest observed germatranes. significant...