A5069992410- Sunflower and Safflower Cultivation
- Metabolomics and Mass Spectrometry Studies
- Leech Biology and Applications
- Mass Spectrometry Techniques and Applications
- Aquaculture disease management and microbiota
- Venomous Animal Envenomation and Studies
- Mitochondrial Function and Pathology
- Microbial infections and disease research
- Advanced Proteomics Techniques and Applications
- Fatty Acid Research and Health
- Bacteriophages and microbial interactions
- Diet and metabolism studies
- Infant Nutrition and Health
- Lipid metabolism and biosynthesis
- Spectroscopy and Chemometric Analyses
- Healthcare and Venom Research
- Metabolism and Genetic Disorders
- Peripheral Neuropathies and Disorders
- Biochemical and Structural Characterization
- Pharmacological Effects and Assays
- Viral Infections and Vectors
- Invertebrate Immune Response Mechanisms
- Advanced Biosensing Techniques and Applications
- Myasthenia Gravis and Thymoma
- Cholesterol and Lipid Metabolism
Skolkovo Institute of Science and Technology
2018-2024
Federal Medical-Biological Agency
2018-2020
Institute of Bioorganic Chemistry
2014-2020
All-Russian Research Institute of Oil Crops by V.S. Pustovoit
2020
Vavilov Institute of General Genetics
2020
Southern California University for Professional Studies
2020
University of Southern California
2020
Russian Academy of Sciences
2011-2018
Federal Research and Clinical Center of Physical-Chemical Medicine named after Y.M. Lopukhin
2016-2017
Abstract Lipids are the most abundant but poorly explored components of human brain. Here, we present a lipidome map brain comprising 75 regions, including 52 neocortical ones. The composition varies greatly among affecting 93% 419 analyzed lipids. These differences reflect brain’s structural characteristics, such as myelin content (345 lipids) and cell type (353 lipids), also functional traits: connectivity (76 information processing hierarchy (60 lipids). Combining lipid mRNA expression...
Ovarian cancer ascites is a native medium for cells that allows investigation of their secretome in natural environment. This interest as promising source potential biomarkers, and also cell–cell communication. The aim this study was to elucidate specific features the malignant metabolome proteome. In order omit components systemic response formation, we compared with cirrhosis ascites. Metabolome analysis revealed 41 differed significantly between Most identified cancer-specific metabolites...
Acute inflammatory demyelinating polyneuropathy (AIDP) - the main form of Guillain-Barre syndrome-is a rare and severe disorder peripheral nervous system with an unknown etiology. One hallmarks AIDP pathogenesis is significantly elevated cerebrospinal fluid (CSF) protein level. In this paper CSF peptidome proteome in were analyzed compared multiple sclerosis control patients. A total concentration increase was shown to be because even changes all proteins rather than some specific response,...
Abstract Background Salivary cell secretion (SCS) plays a critical role in blood feeding by medicinal leeches, making them of use for certain medical purposes even today. Results We annotated the Hirudo medicinalis genome and performed RNA-seq on salivary cells isolated from three closely related leech species, H. medicinalis, orientalis , verbana . Differential expression analysis verified proteomics identified cell-specific gene expression, many which encode previously unknown components....
Several studies have described functional peptides encoded in RNA that are considered to be noncoding. Telomerase together with telomerase reverse transcriptase and regulatory proteins make up the complex, major component of telomere length-maintaining machinery. In contrast protein subunits, is expressed constitutively most somatic cells where absent. We show here transcript human codes a 121 amino acid (hTERP). The existence hTERP was shown by immunoblotting, immunofluorescence microscopy...
Sunflower is an important oilseed crop domesticated in North America approximately 4000 years ago. During the last century, oil content sunflower was under strong selection. Further improvement of properties achieved by modulating its fatty acid composition one main directions modern breeding.We searched for genetic basis variation genotyping 601 inbred lines and assessing their lipid composition. Our genome-wide association analysis based on genotypes 15,483 SNPs concentrations 23 acids,...
Abstract What strategies do bacteria employ for adaptation to their hosts and are these different varied hosts? To date, many studies on the interaction of bacterium its host have been published. However, global changes in bacterial cell process invasion persistence, remain poorly understood. In this study, we demonstrated phase transition avian pathogen Mycoplasma gallisepticum upon various types eukaryotic cells (human, chicken, mouse) which was stable during several passages after...
Sunflower and rapeseed are among the most important sources of vegetable oil for food industry. The main components triglycerides (TAGs) (about 97%). Ultra- performance liquid chromatography coupled with mass spectrometry (UPLC⁻MS) profiling TAGs in sunflower has been performed TAG profiles obtained these species have compared. It identified that 34 shared by rapeseed. was demonstrated 52:2, 52:5, 52:6, 54:3; 54:4, 54:7, 56:3, 56:4, 56:5 had highest variability levels between higher presence...
As essential conservative component of the innate immune systems living organisms, antimicrobial peptides (AMPs) could complement pharmaceuticals that increasingly fail to combat various pathogens exhibiting increased resistance microbial antibiotics. Among properties AMPs suggest their potential as therapeutic agents, diverse in venoms predators demonstrate activity and kill a wide range microorganisms. To identify potent AMPs, study reported here involved transcriptomic profiling tentacle...
Abstract Background Lipids contained in milk are an essential source of energy and structural materials for a growing neonate. Furthermore, lipids’ long-chain unsaturated fatty acid residues can directly participate neonatal tissue formation. Here, we used untargeted mass spectrometric measurements to assess lipid composition seven mammalian species: humans, two macaque species, cows, goats, yaks, pigs. Results Analysis the main class, triacylglycerides (TAGs), revealed species-specific...
Plant protoplasts are widely used for genetic manipulation and functional studies in transient expression systems. However, little is known about the molecular pathways involved a cell response to combined stress factors resulted from protoplast generation. Plants often face more than one type of at time, how plants respond therefore great interest. Here, we moss Physcomitrella patens as model study effects short-term on chloroplast proteome. Using label-free comparative quantitative...
Blood as connective tissue potentially contains evidence of all processes occurring within the organism, at least in trace amounts (Petricoin et al., 2006) [1]. Because their small size, peptides penetrate cell membranes and epithelial barriers more freely than proteins. Among found blood, there are both fragments proteins secreted by various tissues performing function plasma receptor ligands: hormones, cytokines mediators cellular response (Anderson 2002) [2]. In addition, minor amounts,...
Sea anemones (Actiniaria) are intensely popular objects of study in venomics. Order Actiniaria includes more than 1,000 species, thus presenting almost unlimited opportunities for the discovery novel biologically active molecules. The venoms cold-water sea studied far less tropical anemones. In this work, we analysed molecular venom composition anemone Cnidopus japonicus. Two sets NGS data from two species revealed molecules belonging to a variety structural classes, including neurotoxins,...
Numerous brain imaging studies have reported white matter alterations in schizophrenia, but the lipidome analysis of corresponding tissue remains incomplete. In this study, we investigated composition six subcortical regions to major axonal tracks both control subjects and schizophrenia patients. All exhibited a consistent pattern quantitative involving myelin-forming mitochondria associated lipid classes. While alteration levels lipids, particularly sphingolipids, aligned with extent myelin...
Despite a large number of proteomic studies biological fluids from ovarian cancer patients, there is lack sensitive screening methods in clinical practice (Kim et al., 2016) (DOI:https://doi.org/10.1111/cas.12987 [1]). Low molecular weight endogenous peptides more easily diffuse across endothelial barriers than proteins and can be relevant biomarker candidates (Meo (DOI:https://doi.org/10.18632/oncotarget.8931 [2], (Bery 2014) DOI:https://doi.org/10.1186/1559-0275-11-13 [3], (Huang 2018)...
Abstract Despite the fact term “proteome” was proposed to characterize a set of proteins in one mycoplasma species, proteome response various exposures this bacteria are still obscure. Commonly, authors studying proteomic on perturbation models mycoplasmas use single approach and do not confirm their findings by alternative methods. Consequently, results analysis should be validated complementary techniques. In study we utilized three approaches (SWATH, MRM, 2D-DIGE) assess Mycoplasma...
Oilseed crops are one of the most important sources vegetable oils for food and industry. Nutritional technical properties oil primarily determined by its fatty acid (FA) composition. The content composition FAs in plants commonly using gas chromatography-mass spectrometry (GS-MS) or chromatography-flame ionization detection (GC-FID) techniques. In present work, we applied ultra-performance liquid (UPLC-MS) technique to FA profiling sunflower rapeseed seeds compared this method with GC-FID...
INTRODUCTION: Schizophrenia, although a debilitating mental illness, greatly affects individuals physical health as well. One of the leading somatic comorbidities associated with schizophrenia is cardiovascular disease, which has been estimated to be one causes excess mortality in patients diagnosed schizophrenia. Although shared susceptibility and disease well established, mechanisms linking these two disorders are not understood. Genetic studies have hinted toward lipid metabolism...
Abstract Salivary cell secretion (SCS) plays a critical role in blood feeding by medicinal leeches, making them of use for certain medical purposes even today. We annotated the Hirudo medicinalis genome and performed RNA-seq on salivary cells isolated from three closely related leech species, H. medicinalis, orientalis, verbana. Differential expression analysis verified proteomics identified cell-specific genes, many which encode previously unknown components. However, genes encoding known...
Abstract Background Proteomics of bacterial pathogens is a developing field exploring microbial physiology, gene expression and the complex interactions between bacteria their hosts. One complications in proteomic approach micro- macro-heterogeneity species, which makes it impossible to build comprehensive database genomes for identification, while most existing algorithms rely largely on genomic data. Results Here we present large scale study identification single amino acid polymorphisms...
The data reported is a large-scale untargeted proteome profile for Mycoplasma gallisepticum - model organism studying both regulation in genome-reduced bacteria and intracellular infection (Mazin et al., 2014) [1,2]. While seminal whole-proteome studies were performed on genitalium [3] few datasets are available pneumoniae, no data-independent (DIA) profiling has been published of genus. Since DIA-based allows to extract evidence presence quantity any protein interest post-acquisition manner...