A5034550398- Monoclonal and Polyclonal Antibodies Research
- Immunotherapy and Immune Responses
- Multiple Sclerosis Research Studies
- T-cell and B-cell Immunology
- Glycosylation and Glycoproteins Research
- Advanced Biosensing Techniques and Applications
- RNA Interference and Gene Delivery
- Extracellular vesicles in disease
- Advanced biosensing and bioanalysis techniques
- Cytomegalovirus and herpesvirus research
- Immune Response and Inflammation
- Protein Tyrosine Phosphatases
- Toxin Mechanisms and Immunotoxins
- Systemic Lupus Erythematosus Research
- Polyomavirus and related diseases
- Ubiquitin and proteasome pathways
- Salivary Gland Disorders and Functions
- Herpesvirus Infections and Treatments
- Oral microbiology and periodontitis research
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- COVID-19 epidemiological studies
- Diabetes and associated disorders
- DNA and Nucleic Acid Chemistry
- Viral Infections and Vectors
Institute of Bioorganic Chemistry
2015-2024
Institute of Bioorganic Chemistry
2018-2024
Kazan Federal University
2016-2019
Russian Academy of Sciences
2015-2016
Multiple sclerosis (MS) is a widespread neurodegenerative autoimmune disease with unknown etiology. It increasingly evident that, together pathogenic T cells, autoreactive B cells are among the major players in MS development. The analysis of myelin neuroantigen-specific antibody repertoires and their possible cross-reactivity against environmental antigens, including viral proteins, could shed light on mechanism induction progression. A phage display library single-chain variable fragments...
During the ongoing coronavirus disease 2019 (COVID-19) pandemic, many individuals were infected with and have cleared virus, developing virus-specific antibodies effector/memory T cells. An important unanswered question is what levels of T-cell antibody responses are sufficient to protect from infection.
Acute inflammatory demyelinating polyneuropathy (AIDP) - the main form of Guillain-Barre syndrome-is a rare and severe disorder peripheral nervous system with an unknown etiology. One hallmarks AIDP pathogenesis is significantly elevated cerebrospinal fluid (CSF) protein level. In this paper CSF peptidome proteome in were analyzed compared multiple sclerosis control patients. A total concentration increase was shown to be because even changes all proteins rather than some specific response,...
Recent findings indicate that the ubiquitin-proteasome system is involved in pathogenesis of cancer as well autoimmune and several neurodegenerative diseases, thus a target for novel therapeutics. One disease related to aberrant protein degradation multiple sclerosis, an disorder involving processing presentation myelin autoantigens leads destruction axons. Here, we show brain-derived proteasomes from SJL mice with experimental encephalomyelitis (EAE) ubiquitin-independent manner generate...
Multiple sclerosis (MS) is an autoimmune chronic inflammatory disease of the central nervous system (CNS). Cross-reactivity neuronal proteins with exogenous antigens considered one possible mechanisms MS triggering. Previously, we showed that monoclonal myelin basic protein (MBP)-specific antibodies from patients cross-react Epstein-Barr virus (EBV) LMP1. In this study, report exposure mice to LMP1 results in induction myelin-reactive autoantibodies vivo. We posit or multiple acute exposures...
Previously, we showed that CD206-targeted liposomal delivery of co-encapsulated immunodominant myelin basic protein (MBP) sequences MBP
Since periodontitis and type 2 diabetes mellitus are complex diseases, a thorough understanding of their pathogenesis requires knowing the relationship these pathologies with other disorders environmental factors. In this study, representability subgingival periodontal microbiome 46 subjects was studied by 16S rRNA gene sequencing shotgun pooled samples. We examined 15 patients chronic (CP), associated (CPT2DM), 16 healthy (Control). The severity generalized in both groups (CP CPT2DM)...
Identifying high-affinity antibodies in human serum is challenging due to extremely low number of circulating B cells specific the desired antigens. Delays caused by a lack information on immunogenic proteins viral origin hamper development therapeutic antibodies. We propose an efficient approach allowing for enrichment against pathogen with simultaneous epitope mapping, even absence structural about pathogenic immunogens. To screen from blood recovered donors, only transcriptome required...
We have previously shown that immunodominant MBP peptides encapsulated in mannosylated liposomes (Xemys) effectively suppressed experimental allergic encephalomyelitis (EAE). Within the frames of successfully completed phase I clinical trial, we investigated changes serum cytokine profile after Xemys administration MS patients. observed a statistically significant decrease MCP-1/CCL2, MIP-1 β /CCL4, IL-7, and IL-2 at time study completion. In contrast, levels TNF- α were remarkably elevated....
Delivering protein therapeutics specifically into target cells and tissues is a promising avenue in medicine. Advancing this process will significantly enhance the efficiency of designed drugs. In regard, natural membrane-based systems are particular interest. Extracellular vesicles (EVs), being bilayer lipid particles secreted by almost all types cells, have several principal advantages: biocompatibility, carrier stability, blood–brain barrier penetrability, which make them perspective tool...
Antibody-independent B cell effector functions play an important role in the development and suppression of immune response. An extensive body data on cytokine regulation response by lymphocytes has been accumulated over past fifteen years. In this review, we focused mechanisms inflammatory subpopulations regulatory cells health autoimmune pathologies.
The mechanisms triggering most of autoimmune diseases are still obscure. Autoreactive B cells play a crucial role in the development such pathologies and, particular, production autoantibodies different specificities. combination deep-sequencing technology with functional studies antibodies selected from highly representative immunoglobulin combinatorial libraries may provide unique information on specific features repertoires autoreactive cells. Here, we have analyzed cross-combinations...
The possibility of targeted drug delivery to a specific tissue, organ, or cell has opened new promising avenues in treatment development. technology aims create multifunctional carriers that are capable long circulation the patient's organism and possess low toxicity at same time. surface modern synthetic high structural similarity membrane, which, when combined with additional modifications, also promotes transfer biological properties order penetrate physiological barriers effectively....
Periodontal diseases, especially those with polymicrobial etiology, are often associated type 2 diabetes mellitus, proceeding more severely and affecting the course of mellitus. Recently, this feature has been ability periodontopathogen microflora to cause not only a local infectious process in oral cavity, but also interact human immune system induce various systemic effects. We investigated changes salivary cytokine profile patients chronic periodontitis, observed statistically significant...
Background B lymphocytes play a pivotal regulatory role in the development of immune response. It was previously shown that deficiency cells (Bregs) or decrease their anti-inflammatory activity can lead to immunological dysfunctions. However, exact mechanisms Bregs and functioning are only partially resolved. For instance, little is known about structure cell receptor (BCR) repertoires autoimmune disorders, including multiple sclerosis (MS), severe neuroinflammatory disease with yet unknown...
The regulatory functions of the B-cell compartment play an important role in development and suppression immune response. Disruption their anti-inflammatory may lead to acceleration immunopathological processes, autoimmune diseases, particular. Unfortunately, exact mechanism underlying functioning B cells (Breg) has not yet been fully elucidated. Almost nothing is known about specificity structure receptors (BCRs). In this research, we analyzed BCR repertoire transitional Breg (tBreg)...
Abstract Various pathological processes are known to be associated with the production of IgG autoantibodies, which have high affinity for self-antigens and often cause tissue injury development autoimmune diseases. However, mechanism their cytotoxic activity is not clearly understood yet. Here, we shown that action these autoantibodies on cells expressing TNFR1 (the cell surface receptor TNFα) can both caspase-dependent apoptosis necroptosis cells, suppression resulting in switching...
Abstract Multiple sclerosis (MS) is an autoimmune neurodegenerative disease leading to inevitable disability and primarily affecting the young middle-aged population. Recent studies have shown a direct correlation between risk of MS development Epstein–Barr virus (EBV) infection. Analysis titer EBV-specific antibodies among patients with healthy donors Russian population confirmed that characterized by increased level serum IgG binding EBNA-1 (EBV nuclear antigen 1). The number elevated...
Multiple sclerosis (MS) is a neuroinflammatory disease triggered by combination of genetic traits and external factors. Autoimmune nature MS proven the identification pathogenic T cells, but role autoantibody-producing B cells less clear. A comprehensive understanding development neuroinflammation targeted autoantigens are crucial for timely diagnosis appropriate treatment.
Targeting protein therapeutics to specific cells and tissues is a major challenge in modern medicine. Improving the specificity of therapeutic delivery will significantly enhance efficiency drug development. One promising tools for extracellular vesicles (EVs) that are enveloped by complex lipid bilayer. EVs secreted almost all cell types possess significant advantages: biocompatibility, stability, ability penetrate blood-brain barrier. Overexpression vesicular stomatitis virus G (VSV-G) was...
Multiple sclerosis (MS) is an autoimmune disease characterized by demyelination and consequent neuron injury. Although the pathogenesis of MS largely unknown, a breach in immune self-tolerance to myelin followed development autoreactive encephalitogenic T cells suggested play central role. The basic protein (MBP) believed be one main targets for lymphocytes. Recently, immunodominant MBP peptides encapsulated into mannosylated liposomes, referred as Xemys, were shown suppress experimental...
Specific recognition of ligands by surface receptors eukaryotic cells is a fundamental process in sensing the exogenous environment, including cell-to-cell communication. These interactions are therefore widely probed both basic studies and drug development to enhance or interrupt them. Here, we designed high-throughput publicly available platform for visualization selection according specificity surface-exposed consolidation phage display flow cytometry techniques. Polypeptide membrane...
Significance DNA libraries are predisposed to template mispairing during conventional “bulk” PCR, leading the loss of unique sequences. The latter is facilitated by nonuniform distribution templates frequently observed in libraries. These effects result a prominent reduction original diversity. encapsulation repertoires liquid droplets abolishes fundamental advantages emulsion PCR (ePCR) over bulk illustrated deep sequencing and mathematical modeling, which provide general strategy for ePCR...
Summary Rapid spread of COVID-19 pandemic made a substantial share the world population immunised by SARS-CoV-2 antigens. Infection induces development virus-specific antibodies and T cells. Ample evidence on antibody-mediated protection is contrasted elusive role cells in preventing infection. To explore impact to quantify protective levels immune responses we conducted large prospective study: 5,340 Moscow residents were evaluated for antibody cellular monitored up 300 days. The tightly...