A5056388155- Monoclonal and Polyclonal Antibodies Research
- Chemical Synthesis and Analysis
- Protein purification and stability
- Glycosylation and Glycoproteins Research
- Renal Diseases and Glomerulopathies
- Asymmetric Synthesis and Catalysis
- Click Chemistry and Applications
- Epigenetics and DNA Methylation
- Synthetic Organic Chemistry Methods
- Chemical Synthesis and Reactions
- Asymmetric Hydrogenation and Catalysis
- Synthesis and Biological Evaluation
- Radical Photochemical Reactions
- Biotin and Related Studies
- Genomics and Chromatin Dynamics
- Mast cells and histamine
- Advanced Synthetic Organic Chemistry
- Organoboron and organosilicon chemistry
- Inorganic and Organometallic Chemistry
- Cholinesterase and Neurodegenerative Diseases
- Peptidase Inhibition and Analysis
- Immunotherapy and Immune Responses
- RNA modifications and cancer
- Complement system in diseases
- Nicotinic Acetylcholine Receptors Study
University of Campania "Luigi Vanvitelli"
2020-2023
University of Naples Federico II
2018-2019
Institute for Experimental Endocrinology and Oncology
2017
University of California, Davis
2004-2014
Pediatric Nephrology of Alabama
1977-2009
University of California System
2007-2009
Verband der Chemischen Industrie
2004
Russian Academy of Sciences
2000-2002
The University of Texas at Austin
2002
The University of Texas Health Science Center at Houston
1998-2001
Monoclonal antibodies elicited to haptens that are analogs of the transition state for hydrolysis carboxylic esters behaved as enzymic catalysts with appropriate substrates. These substrates distinguished by structural congruence both products haptenic fragments. The were potent inhibitors this esterolytic activity, in agreement their classification analogs. Mechanisms proposed account different chemical behavior these two types ester generation an artificial enzyme through stabilization was...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTReduction of .alpha.,.beta.-acetylenic ketones with B-3-pinanyl-9-borabicyclo[3.3.1]nonane. High asymmetric induction in aliphatic systemsM. Mark Midland, Deborah C. McDowell, Robert L. Hatch, and Alfonso TramontanoCite this: J. Am. Chem. Soc. 1980, 102, 2, 867–869Publication Date (Print):January 1, 1980Publication History Published online1 May 2002Published inissue 1 January...
A monoclonal antibody elicited by a transition-state analog that is representative of an intramolecular six-membered ring cyclization reaction acted as stereospecific, enzyme-like catalyst for the appropriate substrate. Formation single enantiomer δ-lactone from corresponding racemic δ-hydroxyester was accelerated about factor 170, which permitted isolation lactone in enantiomeric excess 94 percent. This finding demonstrates feasibility catalytic-antibody generation chemical transformations...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTChiral trialkylborane reducing agents. Preparation of 1-deuterio primary alcohols high enantiomeric purityM. Mark Midland, Sue Greer, Alfonso Tramontano, and Stephen A. ZdericCite this: J. Am. Chem. Soc. 1979, 101, 9, 2352–2355Publication Date (Print):April 1, 1979Publication History Published online1 May 2002Published inissue 1 April 1979https://pubs.acs.org/doi/10.1021/ja00503a019https://doi.org/10.1021/ja00503a019research-articleACS...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTotal synthesis of the quinonoid alcohol dehydrogenase coenzyme (1) methylotrophic bacteriaE. J. Corey and Alfonso TramontanoCite this: Am. Chem. Soc. 1981, 103, 18, 5599–5600Publication Date (Print):September 1, 1981Publication History Published online1 May 2002Published inissue 1 September 1981https://pubs.acs.org/doi/10.1021/ja00408a067https://doi.org/10.1021/ja00408a067research-articleACS PublicationsRequest reuse permissionsArticle...
Monoaryl phosphonate esters, designated as analogs of the transition state in hydrolysis carboxylic were synthesized and used haptens to generate specific monoclonal antibodies. Some these antibodies react with cognate aryl esters release a fluorescent alcohol. The reaction appears be stoichiometric; however, activity is slowly regenerated under alkaline conditions or by treatment hydroxylamine. Specificity rigorous for p-trifluoroacetamidophenylacetic acid, demonstrating structural...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTSynthesis of alkyl 4-hydroxy-2-alkynoatesM. Mark Midland, Alfonso Tramontano, and John R. CableCite this: J. Org. Chem. 1980, 45, 1, 28–29Publication Date (Print):January 1980Publication History Published online1 May 2002Published inissue 1 January 1980https://pubs.acs.org/doi/10.1021/jo01289a006https://doi.org/10.1021/jo01289a006research-articleACS PublicationsRequest reuse permissionsArticle Views1766Altmetric-Citations85LEARN ABOUT THESE...
The Thyroid Hormone (TH) activating enzyme, type 2 Deiodinase (D2), is functionally required to elevate the TH concentration during cancer progression advanced stages. However, mechanisms regulating D2 expression in still remain poorly understood. Here, we show that cell stress sensor and tumor suppressor p53 silences expression, thereby lowering intracellular THs availability. Conversely, even partial loss of elevates D2/TH resulting stimulation increased fitness cells by boosting a...
The concept of “internal image” antiidiotypic antibodies has provided the basis for eliciting catalytic antibodies. A monoclonal IgM 9A8 that was obtained as an antiidiotype to AE-2 mAb, a known inhibitor acetylcholinesterase, displayed esterolytic activity. Study recombinant Fab fragments and separate light heavy chains confirmed antibody variable domain encodes function, whereas neither part primary sequence exhibited homology with enzyme. specific modification by active site-directed...
Prothrombin is the precursor of thrombin, a central enzyme in coagulation. Autoantibodies to prothrombin are associated with thromboembolism, but mechanisms by which antibodies modulate coagulation processes not understood. We screened panel 34 monoclonal antibody light chains isolated from patients multiple myeloma for prothrombinase activity an electrophoresis method. Two were identified, and one was characterized further. The eluted gel-filtration column run denaturing solvent (6 M...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAntibody catalysis approaching the activity of enzymesAlfonso. Tramontano, Adrian A. Ammann, and Richard LernerCite this: J. Am. Chem. Soc. 1988, 110, 7, 2282–2286Publication Date (Print):March 1, 1988Publication History Published online1 May 2002Published inissue 1 March 1988https://pubs.acs.org/doi/10.1021/ja00215a045https://doi.org/10.1021/ja00215a045research-articleACS PublicationsRequest reuse permissionsArticle...
Multiple sclerosis (MS) is a widespread neurodegenerative autoimmune disease with unknown etiology. It increasingly evident that, together pathogenic T cells, autoreactive B cells are among the major players in MS development. The analysis of myelin neuroantigen-specific antibody repertoires and their possible cross-reactivity against environmental antigens, including viral proteins, could shed light on mechanism induction progression. A phage display library single-chain variable fragments...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTotal synthesis of bongkrekic acidE. J. Corey and Alfonso TramontanoCite this: Am. Chem. Soc. 1984, 106, 2, 462–463Publication Date (Print):January 1, 1984Publication History Published online1 May 2002Published inissue 1 January 1984https://pubs.acs.org/doi/10.1021/ja00314a056https://doi.org/10.1021/ja00314a056research-articleACS PublicationsRequest reuse permissionsArticle Views1000Altmetric-Citations61LEARN ABOUT THESE METRICSArticle Views are...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPreparation of optically active benzyl-.alpha.-d alcohol via reduction by B-3.alpha.-pinanyl-9-borabicyclo[3.3.1]nonane. A new highly effective chiral reducing agentM. Mark Midland, Alfonso Tramontano, and Stephen A. ZdericCite this: J. Am. Chem. Soc. 1977, 99, 15, 5211–5213Publication Date (Print):June 1, 1977Publication History Published online1 May 2002Published inissue 1 June...
Igs offer a versatile template for combinatorial and rational design approaches to the de novo creation of catalytically active proteins. We have used covalent capture selection strategy identify biocatalysts from within human semisynthetic antibody variable fragment library that uses nucleophilic mechanism. Specific phosphonylation at single tyrosine light-chain framework was confirmed in recombinant IgG construct. High-resolution crystallographic structures unmodified phosphonylated Fabs...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTAntibody catalysis of bimolecular amide formationK. D. Janda, R. A. Lerner, and Alfonso. TramontanoCite this: J. Am. Chem. Soc. 1988, 110, 14, 4835–4837Publication Date (Print):July 1, 1988Publication History Published online1 May 2002Published inissue 1 July 1988https://pubs.acs.org/doi/10.1021/ja00222a056https://doi.org/10.1021/ja00222a056research-articleACS PublicationsRequest reuse permissionsArticle Views244Altmetric-Citations52LEARN ABOUT...
Immunization with megalin induces active Heymann nephritis, which reproduces features of human idiopathic membranous glomerulonephritis. Megalin is a complex immunological target four discrete ligand-binding domains (LBDs) that may contain epitopes to pathogenic autoantibodies are directed. Recently, 236-residue N-terminal fragment, termed "L6," spans the first LBD was shown induce and severe disease. We used this model examine epitope-specific contributions pathogenesis. Sera obtained from...
The epithelial-to-mesenchymal transition (EMT) is a complex transcriptional program induced by transforming growth factor β1 (TGF-β1). Histone lysine-specific demethylase 1 (LSD1) has been recognized as key mediator of EMT in cancer cells, but the precise mechanism that underlies activation and repression genes still remains elusive. Here, we characterized early events TGF-β1 during initiation establishment. triggered, 30-90 min post-treatment, nuclear oxidative wave throughout genome,...
The reactivity of phosphonate ester probes with several available proteolytic antibody (Ab) fragments was characterized. Irreversible, active site-directed inhibition the peptidase activity evident. Stable diester-Ab adducts were resolved by column chromatography and denaturing electrophoresis. Biotinylated esters applied for chemical capture phage particles displaying Fv light chain repertoires. Selected Ab displayed enriched catalytic inhibitable selection reagent. Somewhat unexpectedly, a...