A5054424647- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- Chemical Synthesis and Analysis
- Synthesis and Reactions of Organic Compounds
- HIV Research and Treatment
- Synthesis and Biological Evaluation
- Protein purification and stability
- Alzheimer's disease research and treatments
- Chemical Synthesis and Reactions
- Advancements in Solid Oxide Fuel Cells
- Click Chemistry and Applications
- Enzyme Production and Characterization
- Electronic and Structural Properties of Oxides
- Fluorine in Organic Chemistry
- Microbial Metabolites in Food Biotechnology
- Carbohydrate Chemistry and Synthesis
- Rheumatoid Arthritis Research and Therapies
- Synthetic Organic Chemistry Methods
- Magnetic and transport properties of perovskites and related materials
- Analytical Chemistry and Chromatography
- Fuel Cells and Related Materials
- Chronic Lymphocytic Leukemia Research
- T-cell and B-cell Immunology
- Synthesis and biological activity
- Crystallization and Solubility Studies
NTT (Japan)
2009-2025
Kawasaki Hospital
2012-2024
Suzuka University of Medical Science
2011-2024
Kawasaki Municipal Hospital
2018-2023
Kagawa University
2015-2020
Pharmaceutical Biotechnology (Czechia)
2020
Suzuka University
2020
Institute of Systems, Information Technologies and Nanotechnologies
2018
Tottori College
2018
Kyushu University
2004-2018
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNew synthesis of .alpha.,.beta.-unsaturated carboxylic estersKatsuichi Shimoji, Hiroaki Taguchi, Koichiro Oshima, Kisashi Yamamoto, and Hitosi NozakiCite this: J. Am. Chem. Soc. 1974, 96, 5, 1620–1621Publication Date (Print):March 1, 1974Publication History Published online1 May 2002Published inissue 1 March 1974https://pubs.acs.org/doi/10.1021/ja00812a071https://doi.org/10.1021/ja00812a071research-articleACS PublicationsRequest reuse...
To assess the utility of erythrocyte methotrexate-polyglutamate (MTX-PG) concentrations in determining safety and efficacy MTX patients with rheumatoid arthritis (RA).79 MTX-naïve RA were enrolled this prospective 76-week cohort study. was initiated, a predefined dose-escalation protocol followed. Erythrocyte MTX-PG measured using liquid chromatography. The associations disease activity adverse events analysed.Dose escalation resulted increased decrease mean Disease Activity Score 28 joints...
High modulus poly(ethylene terephthalate) (PET)/layered silicate nanocomposites were prepared by employing a novel reactive compatibilizer, 10-[3,5-bis(methoxycarbonyl)phenoxy]decyltriphenylphosphonium bromide (IP10TP), which was designed to link the layered PET matrix through covalent and ionic bonds. The obtained showed 70% higher flexural than raw at maximum.
We describe IgM class human autoantibodies that hydrolyze amyloid beta peptide 1-40 (Abeta40). A monoclonal from a patient with Waldenström's macroglobulinemia hydrolyzed Abeta40 at the Lys-28-Gly-29 bond and Lys-16-Ala-17 bonds. The catalytic activity was inhibited stoichiometrically by an electrophilic serine protease inhibitor. Treatment blocked aggregation toxicity of in neuronal cell cultures. IgMs purified sera patients Alzheimer disease (AD) rates superior to age-matched humans...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPractical synthesis of polyhalomethyllithium carbonyl adductsHiroaki Taguchi, Hisashi Yamamoto, and Hitosi NozakiCite this: J. Am. Chem. Soc. 1974, 96, 9, 3010–3011Publication Date (Print):May 1, 1974Publication History Published online1 May 2002Published inissue 1 1974https://pubs.acs.org/doi/10.1021/ja00816a066https://doi.org/10.1021/ja00816a066research-articleACS PublicationsRequest reuse permissionsArticle Views652Altmetric-Citations74LEARN...
Abstract A general method of transforming a cyclic ketone to the next higher ring homolog chlorinated at α-carbon or alternatively its parent is described. Treatment dichloromethyllithium-carbonyl adduct 2 with butyllithium affords β-oxido carbenoid 3 low temperature. Upon warming, decomposes lithium enolate 4, which quenched diluted hydrochloric acid give α-chloro 5. Cycloalkanone converted one-carbon enlarged halogenated ketone. Meanwhile, dibromomethylcarbinol 7 transformed halogen-free 8...
Many studies are currently investigating the development of safe and effective vaccines to prevent various infectious diseases. Multiple antigen-presenting peptide vaccine systems have been developed avoid adverse effects associated with conventional (i.e., live-attenuated, killed or inactivated pathogens), carrier proteins cytotoxic adjuvants. Recently, two main approaches used develop multiple systems: (1) addition functional components, e.g., T-cell epitopes, cell-penetrating peptides,...
We report the selective catalytic cleavage of HIV coat protein gp120, a B cell superantigen, by IgM antibodies (Abs) from uninfected humans and mice that had not been previously exposed to gp120. The rate IgM-catalyzed gp120 was greater than other polypeptide substrates, including bacterial superantigen A. kinetic parameters varied over broad range depending on source IgMs, turnover numbers as great 2.1/min were observed, suggesting different Abs possess distinct recognition properties. IgG...
Abstract The key step of the title synthesis involves conversion ethyl trimethylsilylacetate to corresponding enolate, 1, by treatment with lithium dicyclohexylamide in tetrahydrofuran at −78 °C. condensation 1 aldehydes and ketones proceeds smoothly afford desired two carbon homologated unsaturated esters excellent yields. new process is extended success readily-enolizable carbonyl compounds also chalcone, which gives no trace Michael adduct present reaction.
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXT.beta.-Oxido carbenoids as synthetic intermediates. Facile ring enlargement reactionHiroaki Taguchi, Hisashi Yamamoto, and Hitosi NozakiCite this: J. Am. Chem. Soc. 1974, 96, 20, 6510–6511Publication Date (Print):October 1, 1974Publication History Published online1 May 2002Published inissue 1 October 1974https://pubs.acs.org/doi/10.1021/ja00827a042https://doi.org/10.1021/ja00827a042research-articleACS PublicationsRequest reuse permissionsArticle...
The title adducts are produced upon treatment of a mixture polyhalomethane and carbonyl compound with hindered lithium amide. kinetically controlled, selective lithiation the halide enables us to avoid troublesome procedure involving preformed carbenoid. components examined dichloromethane, dibromomethane, diiodomethane, chloroform, bromoform, while ones ubiquitous ketones nonanal. A gem-dichloroallyllithium is generated similarly from 3,3-dichloropropene and, furthermore, successfully...
A series of thienylcarbazoles were synthesized by Suzuki-Miyaura and Ullmann coupling reactions. In these compounds, the 2-thienyl or 2,2'-bithiophen-5-yl group is connected at N-, 1,8-, 3,6-, 2,7-, 2,7,N-, 1,8,N-positions carbazole ring. The effects structural variations on their electronic, photophysical, electrochemical properties explored UV-vis fluorescence spectroscopies, cyclic voltammetry (CV), DFT calculations in evaluation potential as material components. thienyl substituents...
The reactivity of phosphonate ester probes with several available proteolytic antibody (Ab) fragments was characterized. Irreversible, active site-directed inhibition the peptidase activity evident. Stable diester-Ab adducts were resolved by column chromatography and denaturing electrophoresis. Biotinylated esters applied for chemical capture phage particles displaying Fv light chain repertoires. Selected Ab displayed enriched catalytic inhibitable selection reagent. Somewhat unexpectedly, a...
We report the chemical activity of immunoglobulin micro and kappa/lambda subunits expressed on surface B cells in secreted IgM antibodies (Abs) found preimmune repertoire. Most nucleophilic reactivity measured by formation covalent adducts a hapten amidino phosphonate diester was attributed to cell receptor. Secreted Abs displayed superior than IgG Abs. catalyzed cleavage model peptide substrates at rates up 344-fold greater Catalytic activities were observed polyclonal from immunologically...
We report the results of efforts to strengthen and direct natural nucleophilic activity antibodies (Abs) for purpose specific cleavage human immunodeficiency virus-1 coat protein gp120. Phosphonate diester groups previously reported form a covalent bond with active site nucleophile serine proteases (Paul, S., Tramontano, A., Gololobov, G., Zhou, Y. X., Taguchi, H., Karle, Nishiyama, Y., Planque, George, S. (2001) J. Biol. Chem. 276, 28314–28320) were placed on Lys side chains Seven...
Accumulation of amyloid-β protein (Aβ) in the brain is thought to be a causal event Alzheimer's disease (AD). Immunotherapy targeting Aβ holds great promise for reducing brain. Here, we evaluated efficacy and safety anti-Aβ single
Nucleophilic sites in the paired variable domains of light and heavy chains (VL VH domains) Ig can catalyze peptide bond hydrolysis. Amyloid beta (Abeta)-binding Igs are under consideration for immunotherapy Alzheimer disease. We searched Abeta-hydrolyzing human IgV (IgVs) a library containing majority single chain Fv clones mimicking physiological VL-VH-combining minority populations with nonphysiological structures generated by cloning errors. Random screening covalent selection...