A5039109039- Mitochondrial Function and Pathology
- Cell death mechanisms and regulation
- RNA Research and Splicing
- ATP Synthase and ATPases Research
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Thermodynamic properties of mixtures
- Ion Transport and Channel Regulation
- Ion channel regulation and function
- Nuclear Structure and Function
- Chemical Thermodynamics and Molecular Structure
- Biochemical and Molecular Research
- Pancreatic function and diabetes
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Drug Transport and Resistance Mechanisms
- RNA Interference and Gene Delivery
- Chemical and Physical Studies
- Glioma Diagnosis and Treatment
- Solid-state spectroscopy and crystallography
- Cynara cardunculus studies
- Paraoxonase enzyme and polymorphisms
- Ubiquitin and proteasome pathways
- Microtubule and mitosis dynamics
- Molecular Biology Techniques and Applications
Institute of Bioorganic Chemistry
2012-2024
Institute of Bioorganic Chemistry
1992-2023
Neurological Surgery
2012-2023
The Ohio State University
2012-2023
The Ohio State University Wexner Medical Center
2023
Mayo Clinic in Arizona
2023
First Affiliated Hospital of Xi'an Jiaotong University
2023
Russian Academy of Sciences
1985-2013
Mayo Clinic
1987-2012
Xi'an Jiaotong University
2012
Ovarian cancer ascites is a native medium for cells that allows investigation of their secretome in natural environment. This interest as promising source potential biomarkers, and also cell–cell communication. The aim this study was to elucidate specific features the malignant metabolome proteome. In order omit components systemic response formation, we compared with cirrhosis ascites. Metabolome analysis revealed 41 differed significantly between Most identified cancer-specific metabolites...
Abstract Purpose: Glioblastoma multiforme (GBM) is a devastating disease. Recent studies suggest that the stem cell properties of GBM contribute to development therapy resistance. Experimental Design: The expression Survivin and Ran was evaluated by immunohistochemistry with tissues, quantitative reverse transcriptase (qRT)-PCR immunocytochemistry patient-derived sphere cultures. With computational structure-based drug design, 11 small-molecule compounds were designed, synthesized, as...
Glioblastoma (GBM) is one of the most aggressive human cancers with a median survival less than two years. A distinguishing pathological feature GBM high degree inter- and intratumoral heterogeneity. Intertumoral heterogeneity has been extensively investigated on genomic, methylomic, transcriptomic, proteomic metabolomics levels, however only few studies describe because lack methods allowing to analyze samples spatial resolution. Here, we applied TOF-SIMS (Time-of-flight secondary ion mass...
Abnormal pre-mRNA splicing regulation is common in cancer, but the effects of chemotherapy on this process remain unclear. To evaluate effect slicing regulation, we performed meta-analyses previously published transcriptomic, proteomic, phosphoproteomic, and secretome datasets. Our findings were verified by LC-MS/MS, western blotting, immunofluorescence, FACS analyses multiple cancer cell lines treated with cisplatin pladienolide B. results revealed that different types lead to similar...
Human ATP1AL1 and corresponding genes of other mammals encode the catalytic α subunit a non‐gastric ouabain‐sensitive H,K‐ATPases, ion pump presumably involved in maintenance potassium homeostasis. The tissue specificity expression these different species has not been analyzed detail. Here we report comparative RT‐PCR screening mouse, rat, rabbit, human, dog tissues. Significant levels were observed skin, kidney distal colon all (with exception human colon). Analysis rat urogenital organs...
The physiological functions of nongastric (colonic) H-K-ATPase (gene symbol Atp12a), unlike those Na-K-ATPase and gastric H-K-ATPase, are poorly understood. It has been suggested that it pumps Na + more efficiently than H ; however, so far, there is no direct evidence in vivo. Previously, we found the α-subunit expressed apical membranes rodent anterior prostate epithelium, a complex with β 1 -subunit. Here report effects Atp12a gene ablation on polarization -subunit secretory function...
PON2 belongs to the paraoxonase protein family that consists of lactone hydrolyzing enzymes with different substrate specificities. Unlike other members family, exhibits substantial antioxidant activity, is localized predominantly inside cell, and ubiquitously expressed in all human tissues. Previously, it was proffered defense against pathogens, such as Pseudomonas aeruginosa, main function paraoxonases. However, recent findings have highlighted important role played by protection oxidative...
We have identified the fifth member of mammalian X,K‐ATPase β‐subunit gene family. The human and rat genes are largely expressed in skeletal muscle at a lower level heart. deduced proteins designated as β (β m ) consist 357 356 amino acid residues, respectively, exhibit 89% identity. sequence homology with known Na,K‐ H,K‐ATPase β‐subunits 30.5–39.4%. Unlike other β‐subunits, putative large N‐terminal cytoplasmic domains containing long Glu‐rich sequences. data obtained indicate existence...
The molecular basis of active ion transport in secretory glands such as the prostate is not well characterized. Rat nongastric H-K-ATPase expressed at high levels distal colon surface cell apical membranes and thus referred to “colonic.” Here we show that ATPase rodent complex a lobe-specific manner. RT-PCR Western blot analyses indicate rat α-subunit (α ng ) mRNA protein are present coagulating gland (anterior prostate) lateral dorsal absent from ventral lobe, whereas Na-K-ATPase all lobes....
The structural organization of nongastric H-K-ATPase, unlike that closely related Na-K-ATPase and gastric is not well characterized. Recently, we demonstrated H-K-ATPase α-subunit (α ng ) expressed in apical membranes rodent prostate. Its highest level, as relative abundance, with respect to α 1 -isoform Na-K-ATPase, was observed anterior lobe. Here, aimed determine the subunit composition through detailed analysis expression all known X-K-ATPase β-subunits rat prostate (AP). RT-PCR detects...
Hemophilia B patients suffer from an inherited blood-clotting defect and require regular administration of factor IX replacement therapy. Recombinant human produced in cultured CHO cells is nearly identical to natural, plasma-derived widely used clinical practice. Development a biosimilar recombinant for medical applications requires the generation clonal cell line with highest specific productivity possible high level procoagulant activity secreted IX. We previously developed plasmid...
In 2020 the world faced pandemic of COVID-19 severe acute respiratory syndrome caused by a new type coronavirus named SARS-CoV-2. To stop spread disease, it is crucial to create molecular tools allowing investigation, diagnoses and treatment COVID-19. One such are monoclonal antibodies (mAbs). this study we describe development hybridoma cells that can produce mouse mAbs against receptor binding domain SARS-CoV-2 spike (S) protein. These able specifically detect native denatured S proteins...
Serine-threonine protein kinases of the DYRK and CLK families regulate a variety vital cellular functions. In particular, these enzymes phosphorylate proteins involved in pre-mRNA splicing. Targeting splicing with pharmacological DYRK/CLK inhibitors emerged as promising anticancer strategy. Investigation pyrido[3,4-g]quinazoline scaffold led to discovery binders differential potency against individual enzyme isoforms. Exploring structure–activity relationship within this chemotype, we...