A5006605423- Bone health and treatments
- Bone Metabolism and Diseases
- Bone health and osteoporosis research
- Connective tissue disorders research
- Phagocytosis and Immune Regulation
- Adenosine and Purinergic Signaling
- Virus-based gene therapy research
- Spaceflight effects on biology
- Cancer Mechanisms and Therapy
- Hepatitis C virus research
- Parathyroid Disorders and Treatments
- Bone Tissue Engineering Materials
- Glioma Diagnosis and Treatment
- Immune cells in cancer
- Radiopharmaceutical Chemistry and Applications
- CAR-T cell therapy research
- Cancer Immunotherapy and Biomarkers
- Hepatitis B Virus Studies
- Orthopaedic implants and arthroplasty
- Cellular Mechanics and Interactions
- Bone and Dental Protein Studies
- Neuroscience of respiration and sleep
- Osteoarthritis Treatment and Mechanisms
- Viral Infectious Diseases and Gene Expression in Insects
- Ion channel regulation and function
Shriners Hospitals for Children - Canada
2016-2025
McGill University
2016-2025
University of Alberta
2024-2025
University of Alabama at Birmingham
2010-2024
McGill University Health Centre
2016-2023
Sechenov University
2023
Ural research Institute of Phthisiopulmonology
2022
Ural State Medical University
2017
Shriners Hospitals for Children - Erie
2012-2015
Royal Victoria Hospital
2014
Basic life science literature is rich with information, however methodically quantitative attempts to organize this information are rare. Unlike clinical research, where consolidation efforts facilitated by systematic review and meta-analysis, the basic sciences seldom use such rigorous methods. The goal of study present a brief theoretical foundation, computational resources workflow outline along working example for performing or rapid reviews research followed meta-analysis. Conventional...
Abstract Natural and genetically modified oncolytic viruses have been systematically tested as anticancer therapeutics. Among this group, conditionally replicative adenoviruses developed for a broad range of tumors with rapid transition to clinical settings. Unfortunately, trials shown limited antitumor efficacy partly due insufficient viral delivery tumor sites. We investigated the possibility using mesenchymal progenitor cells (MPC) virus carriers based on documented tumor-homing abilities...
Abstract Glioblastoma (GBM) is a lethal disease with no effective therapies available. We previously observed upregulation of the TAM (Tyro-3, Axl, and Mer) receptor tyrosine kinase family member AXL in mesenchymal GBM showed that knockdown induced apoptosis mesenchymal, but not proneural, glioma sphere cultures (GSC). In this study, we report BGB324, novel small molecule inhibitor AXL, prolongs survival immunocompromised mice bearing GSC-derived GBM-like tumors. show protein S (PROS1),...
Abstract Intratumor spatial heterogeneity facilitates therapeutic resistance in glioblastoma (GBM). Nonetheless, understanding of GBM is largely limited to the surgically resectable tumor core lesion while seeds for recurrence reside unresectable edge. In this study, stratification and edge demonstrates clinically relevant surgical sequelae. We establish regionally derived models that retain their identity a cell autonomous manner. Upon xenotransplantation, edge-derived cells show higher...
Collagens form the fibrous component of extracellular matrix in all multi-cellular animals. Collagen type I is most abundant collagen present skin, tendons, vasculature, as well organic portion calcified tissue bone and teeth. This review focuses on numerous receptors for which acts a ligand, including integrins, discoidin domain DDR1 2, OSCAR, GPVI, G6b-B Lair-1 leukocyte receptor complex mannose family uPARAP/Endo 180. We explore process production self-assembly, its degradation by...
To evaluate the relationship between osteoblast differentiation and bioenergetics, cultured primary osteoblasts from fetal rat calvaria were grown in medium supplemented with ascorbate to induce differentiation. Before treatment, rate of glucose consumption was 320 nmol · h −1 10 6 cells , respiration 40 ratio lactate production ∼2, indicating that glycolysis main energy source for immature osteoblasts. Ascorbate treatment 14 days led a fourfold increase respiration, threefold ATP...
Systemic acidosis has detrimental effects on the skeleton, and local coincides with bone destruction in inflammatory metastatic diseases. Acidification dramatically enhances osteoclastic resorption, although underlying mechanism remained elusive. We investigated effect of osteoclastogenic transcription factor NFATc1, which upon dephosphorylation translocates from cytoplasm to nuclei. Lowering extracellular pH increased accumulation NFATc1 nuclei rat rabbit osteoclasts levels comparable those...
The Src substrate associated in mitosis of 68 kDa (Sam68) is a KH-type RNA binding protein that has been shown to regulate several aspects metabolism; however, its physiologic role remained elusive. Herein we report the generation Sam68-null mice by homologous recombination. Aged Sam68-/- preserved their bone mass, sharp contrast with 12-month-old wild-type littermates which mass was decreased up approximately 75%. In fact, volume virtually indistinguishable from 4-month-old or mice. marrow...
During bone remodeling, bone-resorbing osteoclasts and bone-forming osteoblasts are organized in multicellular units (BMUs), which travel at a rate of 20-40 mum/d for 6-12 mo, maintaining cylindrical structure. However, the interplay local BMU geometry with biochemical regulation is poorly understood. We developed mathematical model describing changes time space concentrations proresorptive cytokine RANKL its inhibitor osteoprotegerin (OPG), osteoclast osteoblast numbers, mass. assumed that...
Bone metastasis from breast and prostate carcinomas is facilitated by activation of bone-resorbing osteoclasts. Using proteomics approaches, we have identified peroxiredoxin-4 (PRDX4) as a cancer-secreted mediator osteoclastogenesis. We now report characterization L-plastin in the conditioned media (CM) MDA-MB-231 human cancer cells using immunoblotting mass spectrometry. The osteoclastogenic potential CM with siRNA-silenced was significantly reduced. detected cancer-derived exosomes,...
Digital technologies are becoming essential to address and optimize the suboptimal performance of healthcare systems. Teledentistry involves use information communication technology improve access oral health care quality delivery. Several systematic reviews (SRs) have been conducted synthesize evidence on effectiveness teledentistry but with conflicting results. The aim this review is comprehensively summarize available SRs provide impact care, patients’ providers’ outcomes, costs. This...
Mammalian cells represent a novel vector approach for gene delivery that overcomes major drawbacks of viral and nonviral vectors couples cell therapy with delivery. A variety types have been tested in this regard, confirming the ideal cellular system ex vivo has to comply stringent criteria is yet be found. Several properties mesenchymal progenitor (MPCs), such as easy access simple isolation propagation procedures, make these attractive candidates vehicles. In current work, we evaluated...
To restore falling plasma calcium levels, PTH promotes liberation from bone. targets bone-forming cells, osteoblasts, to increase expression of the cytokine receptor activator nuclear factor kappaB ligand (RANKL), which then stimulates osteoclastic bone resorption. Intriguingly, whereas continuous administration decreases mass, intermittent has an anabolic effect on bone, was proposed arise direct effects osteoblastic formation. However, antiresorptive therapies impair ability indicating a...