A5003049858- Plant and fungal interactions
- Gut microbiota and health
- Reproductive tract infections research
- Genomics and Phylogenetic Studies
- Probiotics and Fermented Foods
- Diphtheria, Corynebacterium, and Tetanus
- Clostridium difficile and Clostridium perfringens research
- Bacterial biofilms and quorum sensing
- Mycobacterium research and diagnosis
- Antibiotic Resistance in Bacteria
- Bacterial Identification and Susceptibility Testing
- Toxin Mechanisms and Immunotoxins
- Pneumonia and Respiratory Infections
- Cystic Fibrosis Research Advances
- Infections and bacterial resistance
- Microbial Community Ecology and Physiology
- Bacteriophages and microbial interactions
- Oral microbiology and periodontitis research
- Biochemical and Structural Characterization
- Antibiotics Pharmacokinetics and Efficacy
- Microscopic Colitis
- Veterinary medicine and infectious diseases
- Syphilis Diagnosis and Treatment
- Antimicrobial Resistance in Staphylococcus
- Bacterial Infections and Vaccines
Pirogov Russian National Research Medical University
2016-2025
Federal Medical-Biological Agency
2024-2025
Gabrichevsky Institute of Epidemiology and Microbiology
2020-2023
Russian State University for Physical Education Sport Youth and Tourism
2020
Moscow State Academy of Veterinary Medicine and Biotechnology named after Skryabin
2020
Ministry of Health of the Russian Federation
2020
Yeshiva University
1988
Faecalibacterium prausnitzii is a ubiquitous member of the human gut microbiome, constituting up to 15% total bacteria in gut. Substantial evidence connects decreased levels F. with onset and progression certain forms inflammatory bowel disease, which has been attributed its anti-inflammatory potential. Two phylogroups have identified, decrease phylogroup I being more sensitive marker intestinal inflammation. Much genomic physiological data available date was collected using II strains....
Culture-based study of the faecal microbiome in two adult female subjects revealed presence obligately anaerobic, non-spore-forming, rod-shaped, non-motile, Gram-negative bacterial strains that represent novel species. The first strain, designated 627 T , was a fastidious, slow-growing, indole-positive bacterium with non-fermentative type metabolism. strain characterized by production acetic and succinic acids as metabolic end products, prevalence iso-C 15 : 0 fatty acid menaquinones MK-10...
Two novel strains of Gram-stain-negative, rod-shaped, obligately anaerobic, non-spore-forming, non-motile bacteria were isolated from the faeces healthy human subjects. The strains, designated as 585-1T and 668, characterized by mesophilic fermentative metabolism, production d-lactic acid, succinic acid acetic end products d-glucose fermentation, prevalence C18 : 1ω9, 1ω9 aldehyde, C16 0 1ω7c fatty acids, presence glycine, glutamic lysine, alanine aspartic in petidoglycan peptide moiety lack...
Introduction Bacteroides fragilis (Bf)’s T6SS locus has been characterized and shown to have functional activity in competition experiments. It demonstrated that symbiont nontoxigenic Bf strains a more effective “weapon” effect on pathogenic Bf, which is realized through the of effector-immune (E-I) protein pairs. Intensive study structure led an understanding certain issues related its activity, but exact regulatory mechanisms E-I pair remain unclear. Proteomic annotation components...
A novel obligately anaerobic, non-spore-forming, rod-shaped, non-motile Gram-reaction-negative bacterium was isolated from infant faeces. The strain, designated NSB1(T), able to grow on rich media at 30-37 °C, in the presence of up 2 % (w/v) Oxgall and NaCl. Cells strain NSB1(T) produced catalase, but not urease indole. Aesculin hydrolysed. utilize d-glucose, lactose, maltose, mannose raffinose as electron donors. When grown main metabolic end products were propionic acetic acids, with a...
Members of genus Bifidobacterium are Gram-positive bacteria, representing a large part the human infant microbiota and moderately common in adults. However, our knowledge about their diversity, intraspecific phylogeny long-term persistence humans is still limited. longum generally considered to be most prevalent species intestinal microbiota. In this work we studied whole genome sequences 28 strains B. longum, including 8 described paper. Part these were isolated from healthy children during...
Abstract Background Chlamydia are ancient intracellular pathogens with reduced, though strikingly conserved genome. Despite their parasitic lifestyle and isolated environment, these bacteria managed to avoid accumulation of deleterious mutations leading subsequent genome degradation characteristic for many bacteria. Results We report pan-genomic analysis sixteen species from genus including identification functional annotation orthologous genes, characterization gene gains, losses,...
Microorganisms and their hosts communicate with each other by secreting numerous components. This cross-kingdom cell-to-cell signaling involves proteins small molecules, such as metabolites. These compounds can be secreted across the membrane via transporters may also packaged in outer vesicles (OMVs). Among components, volatile (VOCs) are of particular interest, including butyrate propionate, which have proven effects on intestinal, immune, stem cells. Besides short fatty acids, groups...
A study of species distribution numerically predominant Bacteroidales order isolates in feces healthy people aged 1-33 years was accomplished using a combination amplified ribosomal DNA restriction analysis (ARDRA) and 16S (rDNA) sequencing. It found that the majority all age groups belonged to B. xylanisolvens, vulgatus, uniformis. Members genera Alistipes, Parabacteroides, Odoribacter, Barnesiella, Prevotella were also detected frequently.
A strain of obligately anaerobic, Gram-stain-negative rods was isolated from human faeces and characterized both phenotypically genotypically. Phylogenetic analysis based on 16S rRNA gene whole-genome sequences revealed the to represent a member genus Prevotella, distant species with validly published names, closest relationship Prevotella oryzae. The moderately saccharolytic proteolytic. predominant menaquinones were MK-13 MK-12. major cellular long-chain fatty acids anteiso-C15 : 0 iso-C15...
Three novel strains of Gram-stain-negative, obligately anaerobic, spore-forming straight or slightly curved rods with pointed ends occurring singly in pairs were isolated from the faeces healthy human children. The characterized by mesophilic fermentative metabolism and production acetate, ethanol H2 as end metabolic products. Strains ASD3451 ASD5720T motile, fermented lactose raffinose, weakly maltose. Strain ASD4241T was non-motile did not ferment carbohydrates listed above but starch....
ABSTRACT Inflammatory bowel diseases are characterized by chronic intestinal inflammation and alterations in the gut microbiota composition. Bacteroides fragilis , which secretes outer membrane vesicles (OMVs) with polysaccharide A (PSA), can moderate inflammatory response possibly alter In this study, we created a murine model of sodium dextran sulfate (DSS)-induced colitis treated it B. OMVs. We monitored efficiency OMV therapy determining disease activity index (DAI) performing...
The members of genus Bifidobacterium represent a significant part intestinal microbiota in adults and predominate infants. Species repertoire the bifidobacteria is known to be subjected major changes with age; however, many details this process are still elucidated.Our aim was study diversity their qualitative quantitative composition characteristics during growing up using MALDI-TOF mass-spectrometric analysis ofpure bacterial cultures.A cross-sectional performed 93 healthy people ages from...
A study of the faecal microbiome in three healthy female rhesus macaques revealed presence a novel obligately anaerobic, chemoorganoheterotrophic, non-sporing, coccoid, non-motile, Gram-stain-positive bacterial species. Three strains this species, designated as M108T, M916-1/1, and M919-2/1, were non-haemolytic, H2S-positive, catalase-positive, bile- NaCl-sensitive required peptone for growth. Strains also asaccharolytic, able to utilize sulfite, thiosulfate elemental sulfur electron...
A strain of obligately anaerobic, spore-forming, Gram-positive rods was isolated from child faeces and characterized both phenotypically genotypically. Phylogenetic analysis based on 16S rRNA gene whole genome sequencing revealed the to represent a member family Ruminococcaceae distant described species genera. The moderately saccharolytic with mannose as preferred substrate produced lactic acid, acetic acid H2 end products. major cellular long-chain fatty acids were C16 : 0 aldehyde....
We report the genome sequences of four isolates a human gut symbiont, Bifidobacterium longum. Strains 44B and 35B were isolated from two 1-year-old infants, while 1-6B 2-2B same children 5 years later. The permit investigations factors enabling long-term colonization bifidobacteria.
Invasive pneumococcal disease remains one of the leading causes morbidity and mortality worldwide. In Russia, 13- valent conjugate vaccine (PCV13) was introduced into childhood immunization programme nationwide in 2014. As part Global Pneumococcal Sequencing Project (GPS), we used genome data to characterize 179 isolates collected from Russia 2011-2018 investigate circulating strains using a standardized genomic definition lineages (global sequence clusters, GPSCs), prevalent serotypes...
We report here a draft genome sequence of Megasphaera sp. ASD88, strain from the intestinal microbiota child with autism spectrum disorder, representing previously undescribed species genus Megasphaera. The assembled consists 88 scaffolds, and total size is 2.59 Mb.
In recent decades, nucleic acid sequencing technologies used for metagenomic analysis have become the main methods assessing composition of microbiota. At same time, use novel cultivation and identification microorganisms in microbiological research led to renaissance culture-based technologies, because facilitated discovery isolation both new strains well-known as well uncultivated unexplored bacterial taxa. The aim this study was evaluate potential using method assessment qualitative...
Coprobacter fastidiosus is a Gram-negative obligate anaerobic bacterium belonging to the phylum Bacteroidetes. In this work, we report draft genome sequence of C. strain NSB1(T) isolated from human infant feces.
Antibiotic-resistant strains of Pseudomonas aeruginosa are a global threat to public health. The knowledge mechanisms underlying antibiotic resistance is essential counter P. infections. This study describes the phenomenon meropenem-induced cross-resistance colistin in ATCC 27853 strain aeruginosa. was conducted specimens grown from reference medium containing meropenem gradients. Susceptibility isolates carbapenems and assessed using agar dilution method; susceptibly broth microdilution...
Stenotrophomonas maltophilia is a common opportunistic microorganism and an important respiratory pathogen in cystic fibrosis (CF). The aim of this study was to determine antimicrobial resistance phenotypes, sequence-types (ST) genetic determinants antibiotic S. strains recovered from CF patients Russia. isolates 170 were analyzed. Minimum inhibitory concentrations antibacterial agents determined using Sensititre Gram Negative GNX2F plates the results interpreted according Clinical...