A5062345705- Microbial Community Ecology and Physiology
- Methane Hydrates and Related Phenomena
- Genomics and Phylogenetic Studies
- Climate change and permafrost
- Polar Research and Ecology
- Probiotics and Fermented Foods
- Microbial Metabolites in Food Biotechnology
- Planetary Science and Exploration
- Anaerobic Digestion and Biogas Production
- Geological Studies and Exploration
- Gut microbiota and health
- Advanced Fiber Optic Sensors
- Legume Nitrogen Fixing Symbiosis
- Wastewater Treatment and Nitrogen Removal
- Spaceflight effects on biology
- Metal Extraction and Bioleaching
- Microbial bioremediation and biosurfactants
- Mine drainage and remediation techniques
- Advanced Fiber Laser Technologies
- Geology and Paleoclimatology Research
- Enzyme-mediated dye degradation
- Space Science and Extraterrestrial Life
- Biofuel production and bioconversion
- Environmental Chemistry and Analysis
- Microbial Metabolic Engineering and Bioproduction
G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms
2016-2025
Russian Academy of Sciences
2003-2024
Pushchino Scientific Center for Biological Research
2024
National University of Science and Technology
2024
Perm National Research Polytechnic University
2017-2021
Solikamsk State Pedagogical Institute
2019-2020
Moscow Power Engineering Institute
2019-2020
Japan Aerospace Exploration Agency
2015
Institute of Space and Astronautical Science
2015
Antarctic permafrost soils have not received as much geocryological and biological study has been devoted to the ice sheet, though is more stable older inhabited by microbes. This makes these potentially informative a significant microbial repository than sheets. Due stability of subsurface physicochemical regime, an extreme environment but balanced natural one. Up 104 viable cells/g, whose age presumably corresponds longevity permanently frozen state sediments, isolated from permafrost....
This study describes the biodiversity of indigenous microbial community in sodium-chloride water brines (cryopegs) derived from ancient marine sediments and sandwiched within permafrost 100-120,000 years ago after Arctic Ocean regression. Cryopegs remain liquid at situ temperature -9 to -11 degrees C make up only habitat on Earth that is characterized by permanently subzero temperatures, high salinity, absence external influence during geological time. From these cryopegs, anaerobic aerobic,...
This study summarizes the findings of our research on genesis methane, its content and distribution in permafrost horizons different age origin. Supported by reliable data from a broad geographical sweep, these confirm presence methane permanently frozen fine-grained sediments. In contrast to omnipresence carbon dioxide permafrost, methane-containing (up 40.0mL kg−1) alternate with strata free methane. Discrete representing over tens thousands years are indicative absence diffusion through...
This study describes brine lenses (cryopegs) found in Siberian permafrost derived from ancient marine sediment layers of the Arctic Ocean. The cryopegs were formed and isolated ~100,000-120,000 years ago. They remain liquid at situ temperature -10°C as a result their high salt content (170-300 g/L). [14C] Glucose is taken up by cryopeg biomass -15°C, indicating microbial metabolism low temperatures this habitat. Furthermore, aerobic, anaerobic heterotrophs, sulfate reducers, acetogens,...
Culture-based study of the faecal microbiome in two adult female subjects revealed presence obligately anaerobic, non-spore-forming, rod-shaped, non-motile, Gram-negative bacterial strains that represent novel species. The first strain, designated 627 T , was a fastidious, slow-growing, indole-positive bacterium with non-fermentative type metabolism. strain characterized by production acetic and succinic acids as metabolic end products, prevalence iso-C 15 : 0 fatty acid menaquinones MK-10...
A mesophilic, non-motile, hydrogenotrophic, rod-shaped methanogen, designated M2 T , was isolated from Holocene permafrost sediments of the Kolyma lowland in Russian Arctic. Cells were 3–6 μm long and 0.45–0.5 wide. Strain grew on H 2 /CO formate. Optimum conditions for growth 37 °C, pH 6.8–7.2 0.1 M NaCl. The DNA G+C content 38.1 mol%. On basis 16S rRNA gene sequence comparison with known methanogens, strain affiliated genus Methanobacterium most closely related to veterum MK4 bryantii DSM...
Two novel strains of Gram-stain-negative, rod-shaped, obligately anaerobic, non-spore-forming, non-motile bacteria were isolated from the faeces healthy human subjects. The strains, designated as 585-1T and 668, characterized by mesophilic fermentative metabolism, production d-lactic acid, succinic acid acetic end products d-glucose fermentation, prevalence C18 : 1ω9, 1ω9 aldehyde, C16 0 1ω7c fatty acids, presence glycine, glutamic lysine, alanine aspartic in petidoglycan peptide moiety lack...
A methanogenic archaeon, strain MK4(T), was isolated from ancient permafrost after long-term selective anaerobic cultivation. The cells were rods, 2.0-8.0 microm long and 0.40-0.45 wide, stained Gram-negative. Optimal growth observed at 28 degrees C pH 7.0-7.2 in 0.05 M NaCl. isolate used H(2) plus CO(2), methylamine methanol as sources for methanogenesis. Phylogenetic analysis of the 16S rRNA gene sequence showed close affinity with Methanobacterium bryantii (similarity >99 % to type...
An anaerobic, saccharolytic bacterial strain designated GLS2T was isolated from aggregates of the psychrotolerant archaeon Methanosarcina mazei JL01 arctic permafrost. Bacterial cells were non-motile, spherical, ovoid and annular with diameter 0.2-4 μm. They chemoorganoheterotrophs using a wide range mono-, di- trisaccharides as carbon energy sources. The novel isolate required yeast extract vitamins for growth. bacteria exhibited resistance to number β-lactam antibiotics, rifampicin,...
In the present study, we used culture-independent methods to investigate diversity of methanogenic archaea and their distribution in five permafrost samples collected from a borehole Kolyma River Lowland (north-east Russia). Total DNA was extracted methane-containing different age amplified by PCR. The resulting fragments were cloned. Phylogenetic analysis sequences showed presence all studied samples; 60%–95% belonged Euryarchaeota. Methanogenic novel representatives Methanosarcinales,...
Anaerobic, fermentative, halotolerant bacteria, strains 4-11T and 585, were isolated from production water of two low-temperature petroleum reservoirs (Russia) characterized by using a polyphasic approach. Cells the spherical, non-motile 0.30-2.5 µm in diameter. Strain grew optimally at 35 °C, pH 6.0 1.0-2.0% (w/v) NaCl. Both chemoorganotrophically with mono-, di- trisaccharides. The major cellular fatty acids both C14:0, C16:0, C16:1 ω9 C18:0 3-OH. Major polar lipids glycolipids...
A novel, spore-forming, acidophilic and metal-resistant sulfate-reducing bacterium, strain OL T , was isolated from a microbial mat in tailing dam at gold ore mining site. Cells were slightly curved immotile rods, 0.5 µm diameter 2.0–3.0 long. stained Gram-negative, despite the Gram-positive cell structure revealed by electron microscopy of ultrathin layers. grew pH 4.0–7.0 with an optimum 5.5. utilised H 2 lactate, pyruvate, malate, formate, propionate, ethanol, glycerol, glucose, fructose,...
Abstract Results of an experiment named "Test" on survival and variability microorganisms in open space near the International Space Station are presented. It was found after two-years exposure, spore-forming bacteria species Bacillus subtilis , fungi Aureobasidium pullulans archaea Methanosarcina mazei S-6 T deposited cotton wool, able to survive, their numbers decreased equally, regardless whether belong different taxonomic groups. The main factors for long-term could be result dehydration...
A novel obligately anaerobic, non-spore-forming, rod-shaped, non-motile Gram-reaction-negative bacterium was isolated from infant faeces. The strain, designated NSB1(T), able to grow on rich media at 30-37 °C, in the presence of up 2 % (w/v) Oxgall and NaCl. Cells strain NSB1(T) produced catalase, but not urease indole. Aesculin hydrolysed. utilize d-glucose, lactose, maltose, mannose raffinose as electron donors. When grown main metabolic end products were propionic acetic acids, with a...
The class Limnochordia harbors a single cultivated member, the mesophilic Limnochorda pilosa , which was isolated from meromictic lake. Despite numerous molecular signatures reported in various ecosystems, ecophysiological versatility of this deeply branched lineage Firmicutes (Bacillota) remains poorly understood. objective study to use targeted cultivation, based on metagenome-assembled genomes deep terrestrial aquifer Western Siberia, isolate two new thermophilic members class. These...
A strictly anaerobic, Gram-positive, psychrotolerant, endospore-forming bacterium (strain A121(T)) was isolated from a permafrost sample collected in the Canadian High Arctic. Phylogenetic analysis of 16S rRNA gene sequence strain A121(T) showed its affiliation with group psychrophilic and psychrotolerant members cluster I genus Clostridium, Clostridium bowmanii DSM 14206(T) being closest relative (sequence similarity 98.5 %). Levels DNA-DNA relatedness between type strains phylogenetically...
Polar permafrost is at the forefront of climate change, yet only a few studies have enriched native methane-producing microbes that might provide positive feedbacks to change. Samples Ant1 and Ant2, collected in Antarctic Miers Valley from sediments, with without biogenic methane, respectively, were evaluated for methanogenic activity presence methanogens. After one-year incubation both samples under anaerobic conditions, methane production was observed room temperature microcosm CO2/H2...
A psychrotolerant sulfate-reducing bacterium, designated B15 T , was isolated from supercooled water brine within permafrost of the Varandey Peninsula, on southern coast Barents Sea. Cells were Gram-negative, motile vibrions (3.0–4.0×0.4–0.5 µm) with a single polar flagellum. The isolate positive for desulfoviridin as bisulfite reductase. Strain grew at −2 to 28 °C (optimum 24 °C) and 0–2.0 % NaCl 0.2 %). used H 2 plus acetate, formate, ethanol, lactate, pyruvate choline electron donors...
The terrestrial permafrost represents a range of possible cryogenic extraterrestrial ecosystems on Earth-like planets without obvious surface ice, such as Mars. autotrophic and chemolithotrophic psychrotolerant methanogens are more likely than aerobes to function model for life forms that may exist in frozen subsurface environments Mars, which has no free oxygen, inaccessible organic matter, extremely low amounts unfrozen water. Our research the genesis methane, its content distribution...
A strain of obligately anaerobic, Gram-stain-negative rods was isolated from human faeces and characterized both phenotypically genotypically. Phylogenetic analysis based on 16S rRNA gene whole-genome sequences revealed the to represent a member genus Prevotella, distant species with validly published names, closest relationship Prevotella oryzae. The moderately saccharolytic proteolytic. predominant menaquinones were MK-13 MK-12. major cellular long-chain fatty acids anteiso-C15 : 0 iso-C15...