A5004420600- Microfluidic and Capillary Electrophoresis Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- 3D Printing in Biomedical Research
- Microfluidic and Bio-sensing Technologies
- Neuroscience and Neural Engineering
- Nanopore and Nanochannel Transport Studies
- Neural dynamics and brain function
- Electrowetting and Microfluidic Technologies
- Photonic and Optical Devices
- Advanced Memory and Neural Computing
- Electrochemical Analysis and Applications
- Blood properties and coagulation
- Ion-surface interactions and analysis
- Erythrocyte Function and Pathophysiology
- Electrohydrodynamics and Fluid Dynamics
- Nanofabrication and Lithography Techniques
- Micro and Nano Robotics
- Semiconductor materials and devices
- Semiconductor Quantum Structures and Devices
- Photonic Crystals and Applications
- Mechanical and Optical Resonators
- Orbital Angular Momentum in Optics
- Diatoms and Algae Research
- Lattice Boltzmann Simulation Studies
- Fluid Dynamics and Heat Transfer
Institute for Analytical Instrumentation
2015-2024
Saint Petersburg Academic University
2015-2024
St Petersburg University
2020-2023
Privolzhsky Research Medical University
2021
Siberian Federal University
2016-2017
ITMO University
2011
Russian Academy of Sciences
2011
Significance Biocompatible microfluidic double water-in-oil-in-water emulsion (MDE) enables in-droplet cultivation of different living species. The combination droplet-generating machinery with FACS followed by next-generation sequencing and liquid chromatography-mass spectrometry analysis the secretomes encapsulated organisms yielded detailed genotype/phenotype descriptions. MDE–FACS platform we developed enabled highly sensitive single-cell selection predesigned activity exploration...
The architecture of neuron connectivity in brain networks is one the basic mechanisms by which to organize and sustain a particular function circuitry. There are areas composed well-organized layers neurons connected unidirectional synaptic connections (e.g., cortex, hippocampus). Re-engineering neural circuits with such heterogeneous network structure culture may uncover emergent information functions these circuits. In this study, we present model designed two subpopulations primary...
Significance Successful sperm navigation is essential for sexual reproduction, yet we still understand relatively little about how cells are able to adapt their swimming motion in response chemical and physical cues. This lack of knowledge owed the fact that it has been difficult observe directly full 3D dynamics whip-like flagellum propels cell through fluid. To overcome this deficiency, apply a new algorithm reconstruct beat patterns human experiments under varying flow conditions. Our...
Red blood cells (RBCs) are the most numerous in body and perform gas exchange between all tissues. During infusion of cancer chemotherapeutic (CT) agents, first ones to encounter aggressive cytostatics. Erythrocyte dysfunction caused by direct cytotoxic damage might be a part problem chemotherapy-induced anemia-one frequent side effects. The aim current study is evaluate functional status RBCs exposed mono combinations widely used commercial pharmaceutical CT drugs with different action...
While extracellular vesicles (EVs) are extensively studied by various practical applications in biomedicine, there is still little information on their biomechanical properties due to nanoscale size. We identified isolated blood plasma that carried biomarkers associated with exosomes and exomeres applied atomic force microscopy (AFM) study them at single particle level air liquid. Air measurements of revealed a mechanically indented internal cavity which highly adhesive sites were located....
Abstract Nowadays droplet microfluidics is widely used to perform high throughput assays and for the synthesis of micro- nanoparticles. These applications usually require packaging several reagents into droplets their mixing start a biochemical reaction. For rapid microfluidic devices additional functional elements that make designs more complex. Here we series 2D numerical simulations, followed by experimental studies, introduce novel asymmetric flow-focusing generator, which enhances...
The polyelectrolyte nanocarriers’ based on nanosized vaterite particles as a novel tool for genetic material delivery into the clinically relevant cell types and potential application of described technology in gene therapy approaches.
Abstract Oxidative stress is one of the key factors that leads to red blood cells (RBCs) aging, and impairs their biomechanics oxygen delivery. It occurs during numerous pathological processes causes anaemia, most frequent side effects cancer chemotherapy. Here, we used microfluidics simulate microcirculation RBCs under oxidative induced by tert -Butyl hydroperoxide. was expected make more rigid, which would lead decrease transit velocity in microfluidic channels. However, single-cell...
Introduction: Breast cancer (BC) diagnostics lack noninvasive methods and procedures for screening monitoring disease dynamics. Admitted CellSearch® is used fluid biopsy capture of circulating tumor cells only epithelial origin. Here we describe an RNA aptamer (MDA231) detecting BC in clinical samples, including blood. The MDA231 was originally selected against triple-negative breast cell line MDA-MB-231 using cell-SELEX. Methods: structure solution predicted mFold program molecular dynamic...
Solid-state nanopore devices with plasmonic nanoantennas are powerful tools that allow for working individual particles and molecules in solution as well providing light-induced control of ionic current flow through them. However, the manipulation by light still requires high power densities and, thus, results optical heating nanopore. Here, we solve this problem using a 5 nm gap bow-tie nanoantenna on SiNx membrane irradiated broadband spectrum incident light. In regime, photons not only...
In order to better understand the communication and connectivity development of neuron networks, we designed microfluidic devices with several chambers for growing dissociated neuronal cultures from mice fetal hippocampus (E18). The were connected microchannels providing unidirectional axonal growth between "Source" "Target" neural sub-networks. Experiments performed in a hippocampal plated poly-dimethylsiloxane (PDMS) chip, aligned 60 microelectrode array (MEA). Axonal through was observed...
Abstract The journey of sperm navigation towards ovum is one the most important questions in mammalian fertilisation and reproduction. However, we know very little about spermatozoa propagation a complex fluidic, chemical topographic environment fertility tract. Using microfluidics techniques, investigate influence cell-cell interactions on swimming behavior constrained at different concentrations. Our study shows that high enough cell concentration interaction between boundary-following...
Droplet microfluidics is an extremely useful and powerful tool for industrial, environmental, biotechnological applications, due to advantages such as the small volume of reagents required, ultrahigh-throughput, precise control, independent manipulations each droplet. For generation monodisperse water-in-oil droplets, usually T-junction flow-focusing microfluidic devices connected syringe pumps or pressure controllers are used. Here, we investigated droplet-generation regimes in a device...
Amorphous and microcrystalline GaP films were deposited on Si substrates by time modulated plasma enhanced deposition from trimethylgallium phosphine using constant hydrogen at a temperature of 250–380 °C. obtained low radio-frequency (RF) power (20 W) mode exhibit the broad feature 350–360 cm−1 shoulder 370–390 in Raman spectra. have smooth surface with root-mean-square (RMS) roughness decreasing 0.9 to 0.2 nm increasing 250 380 Small amounts 3–5 nanocrystal inclusions amorphous matrix are...
The aim of this work was to determine a design microfluidic droplet generator which provides the best mixing two components within during its formation. Simulation droplets' formation in various designs generators performed. Dependences index from capillary number and viscosity continuous phase were obtained for each design. determined directly after droplet. It found that small numbers correspond lower due increase droplets diameter. high vortex flows area leads an asymmetric designs, but...
The structured organization of connectivity in neural networks is associated with highly efficient information propagation and processing the brain, contrast disordered homogeneous network architectures. Using microfluidic methods, we engineered modular cultures using dissociated cells unidirectional synaptic connections formed by asymmetric microchannels. complexity microchannel geometry defined strength properties spiking activity propagation. In this study, developed an experimental...
The outstanding optical properties and multiphoton absorption of lead halide perovskites make them promising for use as fluorescence tags in bioimaging applications. However, their poor stability aqueous media biological fluids significantly limits further vitro vivo In this work, we have developed a universal approach the encapsulation perovskite nanocrystals (PNCs) (CsPbBr3 CsPbI3) water-resistant fluorescent markers, which are suitable bioimaging. obtained encapsulated PNCs demonstrate...
Corticosteroids are widely used as an anti-inflammatory treatment for eye inflammation, but the current methods in clinical practice delivery form of drops which is usually complicated patients or ineffective. This results increase risk detrimental side effects. In this study, we demonstrated proof-of-concept research development a contact lens-based system. The sandwich hydrogel lens consists polymer microchamber film made via soft lithography with encapsulated corticosteroid, case,...