A5089602306- Extracellular vesicles in disease
- DNA Repair Mechanisms
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- DNA and Nucleic Acid Chemistry
- Genomics and Chromatin Dynamics
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- RNA and protein synthesis mechanisms
- Chemical Reactions and Isotopes
- Cancer Research and Treatments
- Cancer therapeutics and mechanisms
- Animal Genetics and Reproduction
- Epigenetics and DNA Methylation
- Advanced Proteomics Techniques and Applications
- Advanced Biosensing Techniques and Applications
- Cancer-related Molecular Pathways
- Molecular Biology Techniques and Applications
- RNA Research and Splicing
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Chemical and Physical Studies
- Carcinogens and Genotoxicity Assessment
- Circular RNAs in diseases
- Reproductive Biology and Fertility
- Microtubule and mitosis dynamics
Petersburg Nuclear Physics Institute
2015-2024
Kurchatov Institute
2015-2024
Saint-Petersburg Research Institute of Phthisiopulmonology
2018-2019
Ministry of Health of the Russian Federation
2018-2019
St Petersburg University
2018
National Research Centre
2017
Peter the Great St. Petersburg Polytechnic University
2013-2015
Russian Academy of Sciences
1999-2013
Abstract Background Exosomes are nano-sized vesicles of endocytic origin that involved in cell-to-cell communication including shuttle RNA, mainly mRNA and microRNA. As exosomes naturally carry RNA between cells, these particles might be useful gene cancer therapy to deliver therapeutic short interfering (siRNA) the target cells. Despite promise interference (RNAi) for use therapy, several technical obstacles must overcome. Exogenous siRNA is prone degradation, has a limited ability cross...
BACKGROUND Prostate cancer is the most common in men. Prostate‐specific antigen has, however, insufficient diagnostic specificity. Novel complementary approaches are greatly needed. MiRNAs small regulatory RNAs which play an important role tumorogenesis and being investigated as a biomarker. In addition to their intracellular functions, miRNAs secreted into extracellular space can be found various body fluids, including urine. The stability of defined by association with proteins,...
Thyroid cancer (TC) is the most common endocrine malignancy and its incidence has increased over last few decades. As been revealed by a number of studies, TC tissue's micro-RNA (miRNA) profile may reflect histological features clinical behavior tumor. However, alteration miRNA plasma exosomes associated with development to date not explored. We isolated from assayed their characteristics using laser diffraction particle size analysis, atomic force microscopy, western blotting. Next, we...
Arrangement of chromatin in intact chicken erythrocyte nuclei was investigated by small angle neutron scattering. The scattering spectra have revealed that on the scales between 15 nm and 1.5 μm interior nucleus exhibited properties a mass fractal. fractal dimension protein component cell held constant at approximately 2.5, while DNA organization biphasic, with slightly higher than 2 smaller 300 approaching 3 larger scales.
This study was initiated to determine the relationship between fertilizing potential of spermatozoa and abnormalities in compact packing their chromatin which occurs final stage male germ cell differentiation. Chromatin involves disulphide bridge covalent cross-linking. The degree determined from accessibility DNA a fluorescent dye, ethidium bromide, following detergent treatment spermatozoa. amount dye bound by flow cytometry presence or absence heparin, polyanion removes only...
While extracellular vesicles (EVs) are extensively studied by various practical applications in biomedicine, there is still little information on their biomechanical properties due to nanoscale size. We identified isolated blood plasma that carried biomarkers associated with exosomes and exomeres applied atomic force microscopy (AFM) study them at single particle level air liquid. Air measurements of revealed a mechanically indented internal cavity which highly adhesive sites were located....
Testing of pregnant women reveals weakening neutrophil-mediated effector functions, such as reactive oxygen species generation. This study provides data confirming the phenomenon, gained through application flow cytometry technique. Key factors influencing neutrophil functional activity in blood plasma have not been detected so far. At same time, concentration ceruloplasmin - a copper-containing glycoprotein is known to increase significantly during pregnancy. We observed negative...
Malignant transformation is associated with loss of cell differentiation, anaplasia. Transcription factors gli, required for embryonic development, may be involved in this process. We studied the activity transcription gli high-grade gliomas and their role maintenance stem state glioma survival. 20 lines a sample normal adult brain tissue were used present study. found expression target genes, including GLI1 FOXM1, all tested lines, but not tissue. Interestingly, genes some was observed...
High grade glioma (glioblastoma) is the most common brain tumor. Its malignancy makes it fourth biggest cause of cancer death. In our experiments we used several glioblastoma cell lines generated in laboratory to obtain proteomics information specific for this disease. This study starts developing complete 2DE map proteins. separation with following imaging, immunochemistry, spot picking, and mass-spectrometry allowed us detecting identifying more than 100 Several them have prominent...
The eukaryotic genome consists of chromatin—a nucleoprotein complex with hierarchical architecture based on nucleosomes, the organization higher-order chromatin structures still remains unknown. Available experimental data, including SANS spectra we had obtained for whole nuclei, suggested fractal nature chromatin. Previously built random-walk supernucleosomal models (up to 106 nucleosomes) interpret our spectra. Here report a new method build structure given dimension or two different...
CP is a copper-containing ferroxidase of blood plasma, which acts as an acute phase reactant during inflammation. The effect oxidative modification induced by oxidants produced MPO, such HOCl, HOBr, and HOSCN, on its spectral, enzymatic, anti-inflammatory properties was studied. We monitored the chemiluminescence lucigenin luminol along with fluorescence hydroethidine scopoletin to assay inhibition neutrophilic respiratory burst PMA or fMLP. Superoxide dismutase activity capacity reduce...
<ns4:p><ns4:bold>Background: </ns4:bold>Large protein aggregates, known as circulating immune complexes (CICs), are formed in biological fluids a result of the development body's response to various provoking factors. The kinetic characteristics formation and removal (ICs), their physical parameters, isotypic composition immunoglobulins (Igs) antigenic component CICs may reflect certain aspects pathological metabolic processes taking place humans animals. aim this study is assess that form...
P53 protein is more frequently mutated in human tumours compared with the other proteins. While majority of p53 mutations, especially within its DNA-binding domain, lead to loss wild-type function, there are accumulating data demonstrating that mutants gain tumour promoting activities; latter triggers a revitalised interest functional analysis mutants. A systematic screening for mutations surgical materials from patients glioma revealed 378C>G mutation creates stop codon at position amino...