A5011050819- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Nitric Oxide and Endothelin Effects
- Trace Elements in Health
- Medical and Biological Ozone Research
- Infant Nutrition and Health
- Advanced Nanomaterials in Catalysis
- S100 Proteins and Annexins
- Neurological Disorders and Treatments
- Nanoparticles: synthesis and applications
- Graphene and Nanomaterials Applications
- Iron Metabolism and Disorders
- Vitamin C and Antioxidants Research
- Antimicrobial Peptides and Activities
- Advanced Glycation End Products research
- Vasculitis and related conditions
- Immune Response and Inflammation
- SARS-CoV-2 and COVID-19 Research
- Aquaculture disease management and microbiota
- Pancreatic function and diabetes
- Vanadium and Halogenation Chemistry
- Diabetic Foot Ulcer Assessment and Management
- Heme Oxygenase-1 and Carbon Monoxide
- COVID-19 Clinical Research Studies
- Neonatal Respiratory Health Research
- Neonatal Health and Biochemistry
Institute of Experimental Medicine
2015-2024
Federal Medical-Biological Agency
2012-2024
Czech Academy of Sciences, Institute of Experimental Medicine
2010-2024
Federal Research and Clinical Center of Physical-Chemical Medicine named after Y.M. Lopukhin
2011-2019
Physico-Technical Institute
2014-2016
HUN-REN Institute of Experimental Medicine
2016
Russian Academy of Sciences
2010-2012
Academy of Medical Sciences
2012
Myeloperoxidase (MPO) and eosinophil peroxidase (EPO) are involved in the development of halogenative stress during inflammation. We previously described a complex between MPO ceruloplasmin (CP). Considering high structural homology EPO, we studied latter's interaction with CP checked whether EPO becomes inhibited CP. Disc-electrophoresis gel filtration showed that form stoichiometry 1:1. Affinity chromatography on CP-agarose (150 mM NaCl, 10 Na-phosphate buffer, pH 7.4) resulted retention...
Myeloperoxidase (MPO) is a challenging molecular target which, if put under control, may allow regulating the development of inflammatory reactions associated with oxidative/halogenative stress. In this paper, new kinetic method for assaying halogenating activity MPO described. The based on measuring rate iodide-catalyzed oxidation celestine blue B (CB) by oxygen and taurine N-chloramine (bromamine). latter produced in reaction HOCl (HOBr). CB not substrate peroxidase does react hydrogen...
COVID-19 cases caused by new variants of highly mutable SARS-CoV-2 continue to be identified worldwide. Effective control the spread can achieved through targeting conserved viral epitopes. In this regard, nucleocapsid (N) protein, which is much more than evolutionarily influenced spike protein (S), a suitable antigen. The recombinant N considered not only as screening antigen but also basis for development next-generation vaccines, little known about induction antibodies against via...
Our previous study showed antitumor activity of the complex formed by iron-free recombinant human lactoferrin (apo-recHLF) and oleic acid (1:8 molar ratio) in a model murine hepatoma 22a inoculated to C3HA mice. Taken alone, apo-recHLF was less efficient, i.e., tumor growth index 0.14 for recHLF-8OA 0.63 recHLF as compared with 1.0 control animals. In present we evaluated iron-saturated per se complexes OA holo-recHLF. Balb/c mice solid myeloma Sp2/0 were subjected 10-day treatment daily...
Myeloperoxidase (MPO) is an oxidant-producing enzyme that can also regulate cellular functions via its nonenzymatic effects. Mature active MPO isolated from normal human neutrophils a 145 kDa homodimer, which consists of 2 identical protomers, connected by single disulfide bond. By binding to CD11b/CD18 integrin, dimeric induces neutrophil activation and adhesion augmenting leukocyte accumulation at sites inflammation. This study was performed compare the potency monomeric elicit selected...
The protective effects of recombinant human lactoferrin rhLF (branded "CAPRABEL™") on the cognitive functions rat offspring subjected to prenatal hypoxia (7% O2, 3 h, 14th day gestation) have been analyzed. About 90% in CAPRABEL was iron-free (apo-LF). Rat dams received several injections 10 mg during either gestation (before and after hypoxic attack) or lactation. Western blotting revealed appearance erythropoietin (EPO) alongside hypoxia-inducible factors (HIFs) organ homogenates...
Destruction of ceruloplasmin (Cp) in the presence hydrogen peroxide is accompanied by release protein’s copper ions that provoke formation hydroxyl radicals (OH˙) and, consequently, further degradation protein. Under such conditions, Cp hampered a number substances able to bind ions. Lactoferrin (Lf) most active protector Cp, its protective effect depending on pH medium. The best protection Lf was detected at 7.4. In an acidic buffer (pH 5.5), did not affect destruction Cp. pH-dependent...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to have a significant impact on global public health. Multiple mechanisms for SARS-CoV-2 cell entry been described; however, the role of transferrin receptor 1 (TfR1) in infection has received little attention. We used ferristatin II induce degradation TfR1 surface Vero cells and study consequences such treatment viability replication SARS-CoV-2. demonstrated that is non-toxic concentrations up 400 µM. According confocal...
Due to their extraordinary properties, single-walled carbon nanotubes (SWNTs) have a tremendous potential for medical applications such as clinical diagnostics, targeted drug (or gene) delivery and cancer therapy. Hence, effects of SWNTs on living systems well mechanisms biodegradation SWTNs are great importance must be studied before starting explore use. This study was undertaken compare the different peroxidases in degrading carboxylated SWNT (c-SWNT) elucidate role peroxidase-generated...