A5038435496- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Nitric Oxide and Endothelin Effects
- Medical and Biological Ozone Research
- Infant Nutrition and Health
- Neurological Disorders and Treatments
- Free Radicals and Antioxidants
- Neonatal Respiratory Health Research
- Blood properties and coagulation
- Erythrocyte Function and Pathophysiology
- S100 Proteins and Annexins
- Advanced Glycation End Products research
- Redox biology and oxidative stress
- Neonatal Health and Biochemistry
- Heme Oxygenase-1 and Carbon Monoxide
- Cell Adhesion Molecules Research
- Immune Response and Inflammation
- Vanadium and Halogenation Chemistry
- Molecular Sensors and Ion Detection
- Hemoglobin structure and function
- Vitamin C and Antioxidants Research
- Glycosylation and Glycoproteins Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Lubricants and Their Additives
- Selenium in Biological Systems
- Wound Healing and Treatments
Belarusian State University
2016-2025
Kazan State Technical University named after A. N. Tupolev
2023
Peter the Great St. Petersburg Polytechnic University
2019
Moscow Regional Research Institute of Obstetrics and Gynecology
2003-2017
Myeloperoxidase (MPO) is a heme-containing enzyme released from activated leukocytes into the extracellular space during inflammation. Its main function production of hypohalous acids that are potent oxidants. MPO can also modulate cell signaling and inflammatory responses independently its enzymatic activity. Because regarded as an important risk factor for cardiovascular diseases associated with increased platelet activity, we studied effects on human functional properties. Laser scanning...
Myeloperoxidase (MPO) is an oxidant-producing enzyme that can also regulate cellular functions via its nonenzymatic effects. Mature active MPO isolated from normal human neutrophils a 145 kDa homodimer, which consists of 2 identical protomers, connected by single disulfide bond. By binding to CD11b/CD18 integrin, dimeric induces neutrophil activation and adhesion augmenting leukocyte accumulation at sites inflammation. This study was performed compare the potency monomeric elicit selected...
Hypochlorous acid (HOCl), one of the major precursors free radicals in body cells and tissues, is endowed with strong prooxidant activity. In living systems, dinitrosyl iron complexes (DNIC) glutathione ligands play role nitric oxide donors possess a broad range biological activities. At micromolar concentrations, DNIC effectively inhibit HOCl-induced lysis red blood (RBCs) manifest an ability to scavenge alkoxyl alkylperoxyl generated reaction HOCl tert -butyl hydroperoxide. proved be more...
Myeloperoxidase is a key factor promoting development of halogenative/oxidative stress under inflammatory conditions. Previously, we have discovered complexes including myeloperoxidase and its physiological inhibitor, ceruloplasmin in blood plasma patients with diseases different etiology, e.g., atherosclerosis. Studies on regulation activity by shown that hypochlorous acid, specific product action, likely to modify during inflammation. The present study was aimed for analysis relationships...
The application of vaterite microparticles for mucosal delivery depends on their interaction with mucin and immune cells. As we have shown previously, the binding onto particles enhances generation reactive oxygen species by neutrophils. attenuation pro-oxidant effect bound through modification could improve its biocompatibility. Hybrid composed pectin (CCP) were prepared using co-precipitation. In comparison (CC), they had a smaller diameter pores, greater surface area, negative...
CP is a copper-containing ferroxidase of blood plasma, which acts as an acute phase reactant during inflammation. The effect oxidative modification induced by oxidants produced MPO, such HOCl, HOBr, and HOSCN, on its spectral, enzymatic, anti-inflammatory properties was studied. We monitored the chemiluminescence lucigenin luminol along with fluorescence hydroethidine scopoletin to assay inhibition neutrophilic respiratory burst PMA or fMLP. Superoxide dismutase activity capacity reduce...
Myeloperoxidase (MPO), found mainly in neutrophils, is released inflammation. MPO produces reactive halogen species (RHS), which are bactericidal agents. However, RHS overproduction, i.e., halogenative stress, can also damage host biomolecules, and itself may be targeted by RHS. Therefore, we examined the susceptibility of to inactivation its primary products (HOCl, HOBr, HOSCN) secondary such as taurine monochloramine (TauCl) monobromamine (TauBr). was dose-dependently inhibited up complete...
A significant increase in the myeloperoxidase (MPO) activity has been found plasma of patients with stable angina and acute coronary syndrome (ACS) comparison control group. MPO concentration was significantly increased ACS patients. Reduced treated correlated a favorable outcome disease. Generally, changes coincided lactoferrin thus confirming role neutrophil degranulation concentrations these proteins. The obviously determined by modification protein caused reactive oxygen species halogen...
Hypochlorous acid (HOCl) derived from hydrogen peroxide and chloride anion by myeloperoxidase (MPO) plays a significant role in physiological pathological processes. Herein we report phenoxazine-based fluorescent probe Celestine Blue B (CB) that is applicable for HOCl detection living cells assaying the chlorinating activity of MPO. A remarkable selectivity sensitivity (limit 32 nM), along with rapid "turn-on" response CB to was demonstrated. Furthermore, able detect endogenous reactive...
Hyperglycemia in diabetes mellitus induces modification of proteins by glucose and its derivative methylglyoxal (MG). Neutrophils perform their bactericidal activity mainly via reactive halogen (RHS) oxygen (ROS) species generation catalyzed myeloperoxidase (MPO) stored neutrophil azurophilic granules (AGs) membrane NADPH oxidase, respectively. Herein, we study the binding human serum albumin (HSA) modified with MG (HSA-MG) to MPO effects on release neutrophils. Peroxidase was registered...