A5001804721- Polymer Surface Interaction Studies
- Advanced Drug Delivery Systems
- Proteins in Food Systems
- Protein purification and stability
- Calcium Carbonate Crystallization and Inhibition
- biodegradable polymer synthesis and properties
- Electrospun Nanofibers in Biomedical Applications
- Pickering emulsions and particle stabilization
- Hydrogels: synthesis, properties, applications
- Hair Growth and Disorders
- Glycosylation and Glycoproteins Research
- Enzyme Catalysis and Immobilization
- Enzyme Production and Characterization
- Surfactants and Colloidal Systems
- Infant Nutrition and Health
- Nanoparticle-Based Drug Delivery
- Protein Hydrolysis and Bioactive Peptides
- Nanocomposite Films for Food Packaging
- Drug Solubulity and Delivery Systems
- Silk-based biomaterials and applications
- Electromagnetic Fields and Biological Effects
- Microencapsulation and Drying Processes
- Dyeing and Modifying Textile Fibers
- Blood properties and coagulation
- Analytical Chemistry and Sensors
Lomonosov Moscow State University
2015-2024
Federal Medical-Biological Agency
2018-2024
Moscow State University
2000-2024
Max Planck Institute of Colloids and Interfaces
2003
Stable polyelectrolyte capsules were produced by the layer-by-layer (LbL) assembling of biodegradable polyelectrolytes, dextran sulfate and protamine, on melamine formaldehyde (MF) microcores followed cores decomposition at low pH. The mean diameter pH 3−5 was 8.0 ± 0.2 μm, which is more than that templates (5.12 0.15 μm). With growing up to 7−8, enlarged, swelling 9−10 μm. microcapsules loaded with horseradish peroxidase. Seemingly, peroxidase embedded in gellike structure microcapsule...
Mesoporous vaterite CaCO3 crystals are nowadays one of the most popular vectors for loading fragile biomolecules like proteins due to biocompatibility, high capacity, cost effective and simple procedures. However, recent studies reported reduction bioactivity protein encapsulation into in water rather alkaline pH about 10.3 caused by crystal hydrolysis. In this study we have investigated how retain control release rate enzyme superoxide dismutase (SOD) loaded via co-synthesis. SOD is widely...
Porous vaterite CaCO3 crystals are nowadays extensively used as high-capacity bio-friendly sacrificial templates for the fabrication of such protein-containing nano- and micro-particles capsules beads. The first step in protein encapsulation is performed through loading molecules into crystals. Co-synthesis one most useful simple methods proven to effectively load with proteins; however, mechanism still unknown. To understand mechanism, this study, we focus on a model catalase by means...
Encapsulation of model proteins (catalase, insulin, aprotinin) into multilayer dextran sulphate/protamin capsules by templating on CaCO 3 microparticles is investigated employing: (i) PRE‐loading particles adsorption or co‐synthesis and (ii) POST‐loading performed capsules. Protein encapsulation governed both its size electrostatic interactions with the carbonate shell. enables improved compared to (catalase content in 630 70 mg · g −1 ). Bioactivity encapsulated protein not affected...
Protein therapy gained a reputation of the most direct and safe approach for treating various diseases, yet biodegradation loss bioactivity fragile therapeutic proteins limit their wide medical use. Recently, new hard templating technology using decomposable mesoporous vaterite CaCO3 crystals became extremely popular strategy formulation protein nano(micro)-vectors. This study deciphers how depends on loading/release this utilizing catalase as promising antioxidant agent. Catalase has been...
Multicomponent insulin‐containing microparticles are prepared by layer‐by‐layer assembly of dextran sulfate and chitosan on the core protein‐polyanion complex with or without protease inhibitors. Oral bioavailability encapsulated insulin is improved due to cumulative effect each component. A physico‐chemical study shows that particle design allows adjustment pH‐dependent profile release, as well mucoadhesive properties Ca 2+ binding ability microparticles. Supplementing 2–3% inhibitors fully...
Porous vaterite crystals of CaCO₃ are extensively used for the fabrication self-assembled polymer-based microparticles (capsules, beads, etc.) utilized drug delivery and controlled release. The nature polymer plays a crucial role discovery new perspective biopolymers is essential to assemble with desired characteristics, such as biocompatibility, loading efficiency/capacity, release rate, stability. Glycoprotein mucin tested here good candidate because high charge due sialic acids,...
Vaterite CaCO3 crystals are actively used as a biocompatible and degradable matrix for encapsulation of fragile biomacromolecules. However, the incorporation small cationic drugs into remains awkward due to poor binding these crystal surface scarce retention inside pores. Herein, we achieve efficient drug loading control over release performance via utilisation hybrid impregnated with mucin. The co-loading mucin anticancer doxorubicin (DOX) enhanced content in by ca 12 times giving DOX...
Here we focus on understanding protein interactions with mesoporous CaCO<sub>3</sub>microspheres (vaterite form), which are nowadays very actively used as decomposable templates for biomolecule encapsulation under mild conditions.
Porous vaterite CaCO3 crystals are widely used as containers for drug loading and sacrificial templates to assemble polymer-based nano- micro-particles at mild conditions. Special attention is paid nowadays mucosal delivery where the glycoprotein mucin plays a crucial role main component of mucous. In this work mucoadhesive properties have been tested by investigation binding function (i) time, (ii) concentration, (iii) adsorption conditions (iv) degree desialization. Mucin follows Bangham...
Loading of therapeutic proteins into mesoporous vaterite crystals is driven by inter-protein interactions in bulk solution and inside the crystals.
Poly(N-isopropylacrylamide) (pNIPAM) hydrogels have broad potential applications as drug delivery vehicles because of their thermoresponsive behavior. pNIPAM loading/release performances are directly affected by the gel network structure. Therefore, there is a need with approaches for accurate design 3D assemblies structure ordered at nanoscale. This study demonstrates size-selective spontaneous loading macromolecules (dextrans 10−500 kDa) into microgels microgel heating from 22 to 35 °C...
Catalase is one of the crucial antioxidant enzymes with diverse applications in textile, food industries, wastewater treatment, cosmetics, and pharmaceutics, which, however, highly sensitive to environmental challenges. Resisting loss activity prolongation formulation storage can be achieved via catalase entrapment into insoluble carriers. Affordable degradable vaterite proposed as amicable material for immobilization. To improve carrier properties vaterite, it was co‐precipitated mucin from...
While the enteral delivery of proteolytic enzymes is widely established for combating many diseases as an alternative to antibiotic treatment, their local only emerges administration route enabling sustained release in a controlled manner on site. The latest requires development drug systems suitable encapsulation and preservation enzymatic activity. This study proposes hybrid microspheres made mucin biodegradable porous crystals calcium carbonate (CC) carriers chymotrypsin (CTR) delivery....
Abstract Summary: Polyelectrolyte multilayer microspheres were prepared by alternating adsorption of dextran sulfate and protamine on melamine formaldehyde cores followed the partial decomposition core. Peroxidase glucose oxidase immobilized in microspheres. Retention enzymatic activity peroxidase/glucose system incorporated into was demonstrated. Applicability bienzyme for kinetic assay shown. SEM image polyelectrolyte microsphere. magnified
Microparticles were fabricated by layer-by-layer deposition of chitosan (Ch) and dextran sulfate (DS) on microaggregates formed human insulin DS.Consecutive treatment the negatively charged with Ch, DS, Ch yielded small (ca. 10 μm) positively microparticles high encapsulation efficiency (65% initial amount insulin) loading (50% w/w).Virtually all immobilized protein remained insoluble in pH range 1.0-6.0corresponding to aggressive media stomach upper intestine, while at 7.4, about 90% was...
The growing use of extremely high-frequency electromagnetic radiation (EHF EMR) in information and communication technology biomedical applications has raised concerns regarding the potential biological impact millimeter waves (MMWs). Here, we elucidated effects MMW on neutrophil activation induced by opsonized zymosan or E. coli whole blood ex vivo. After agonist addition to blood, two samples were prepared. A control sample was incubated at ambient conditions without any treatment, a test...