A5071722038- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Medical and Biological Ozone Research
- Antioxidant Activity and Oxidative Stress
- Computational Drug Discovery Methods
- Free Radicals and Antioxidants
- Wound Healing and Treatments
- Nitric Oxide and Endothelin Effects
- Diabetic Foot Ulcer Assessment and Management
- Advanced Nanomaterials in Catalysis
- Redox biology and oxidative stress
- Graphene and Nanomaterials Applications
- Nanoparticles: synthesis and applications
- Advanced Glycation End Products research
- Gut microbiota and health
- Vitamin D Research Studies
- Immune cells in cancer
- Blood disorders and treatments
- Vasculitis and related conditions
- Vitamin C and Antioxidants Research
- Antimicrobial Peptides and Activities
- Metabolomics and Mass Spectrometry Studies
- Infections and bacterial resistance
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Hemoglobin structure and function
- Pancreatitis Pathology and Treatment
Federal Medical-Biological Agency
2012-2024
Research Institute of Physical Chemical Medicine
2016-2021
State Research Institute of Highly Pure Biopreparations
2020
Physico-Technical Institute
2006-2010
Federal Agency for Health and Social Development
2007
Ministry of Health of the Russian Federation
1995-2006
Russian Academy of Sciences
1998
An understanding of the consequences oxidative/halogenative stress triggered by neutrophil activation is impossible without considering NETosis. NETosis, formation extracellular traps (NETs), known to promote microthrombus and impair wound healing in type 2 diabetes mellitus (T2DM) patients. Therefore, there a need search for drugs treatment approaches that could prevent excessive NET formation. We aimed evaluate effect vitamin D3 combination with omega‐3 polyunsaturated fatty acids (vitamin...
Due to their extraordinary properties, single-walled carbon nanotubes (SWNTs) have a tremendous potential for medical applications such as clinical diagnostics, targeted drug (or gene) delivery and cancer therapy. Hence, effects of SWNTs on living systems well mechanisms biodegradation SWTNs are great importance must be studied before starting explore use. This study was undertaken compare the different peroxidases in degrading carboxylated SWNT (c-SWNT) elucidate role peroxidase-generated...
Myeloperoxidase (MPO), found mainly in neutrophils, is released inflammation. MPO produces reactive halogen species (RHS), which are bactericidal agents. However, RHS overproduction, i.e., halogenative stress, can also damage host biomolecules, and itself may be targeted by RHS. Therefore, we examined the susceptibility of to inactivation its primary products (HOCl, HOBr, HOSCN) secondary such as taurine monochloramine (TauCl) monobromamine (TauBr). was dose-dependently inhibited up complete...
Bacteria colonizing human intestine adhere to the gut mucosa and avoid innate immune system. We previously demonstrated that Escherichia coli isolates can adsorb mucin from a diluted solution in vitro. Here, we evaluated effect of adsorption by E. cells on neutrophil activation Activation was based detection reactive oxygen species production chemiluminescent reaction (ChL), observation morphological alterations neutrophils exocytosis myeloperoxidase lactoferrin. report adsorbed SharL1...
Cationic antimicrobial peptides (CAMPs) have gained attention as promising therapeutics causing lower or no bacterial resistance. Considerable achievements been made in designing new CAMPs that are highly active antimicrobials. However, there is a lack of research on their interaction with biologically important proteins. This study focused CAMPs' effects myeloperoxidase (MPO), an enzyme which microbicidal and concomitantly damaging to host biomolecules cells due its ability produce reactive...
Cationic antimicrobial peptides (CAMPs) are considered as next-generation antibiotics with a lower probability of developing bacterial resistance. In view potential clinical use, studies on CAMP biocompatibility important. This work aimed to evaluate the behavior synthetic short CAMPs (designed using bioinformatic analysis medicinal leech genome and microbiome) in direct contact blood cells plasma. Eight were included study. Hemolysis lactate dehydrogenase assays showed that potency disrupt...
Oxidative stress and neutrophil activation leading to an increase in myeloperoxidase (MPO), elastase extracellular trap (NET) levels blood are considered as pathogenic mechanisms responsible for the development of extremity damage people with type 2 diabetes mellitus (T2DM). The aim this study was analyze relationship between factors, associated activation, length initial phase wound healing (the inflammatory phase) T2DM patients. Patients were divided retrospectively into three groups...
This study was carried out to compare the enzymatic and bactericidal activity of mature, dimeric myeloperoxidase (MPO) its monomeric form. Dimeric MPO isolated from HL-60 cells. Hemi-MPO obtained by reductive cleavage a disulfide bond between protomeric subunits used as Both peroxidase halogenating (chlorinating) activities were assayed, each them two methods. Bactericidal MPO/Н2О2/Cl- system tested using Escherichia coli laboratory strain DH5a. No difference in hemi-MPO found. forms enzyme...
In the blood of children (n=16) with large thermal skin burns (> 20% total body surface), luminol-dependent chemiluminescence (CL) neutrophils stimulated phorbol-12-myristate-13-acetate (PMA) and myeloperoxidase (MPO) activity in plasma were assayed early period (1-7 post-burn days). PMA-stimulated thermally injured patients produced higher CL than those a reference group healthy (n=24), p<0.01. MPO was elevated 40% 57% patients' samples, respectively. The albumin fraction isolated from...
Successful colonization of the intestine requires that bacteria interact with innate immune system and, in particular, neutrophils. Progression inflammatory bowel diseases (IBD) is associated alterations gut microbiota, and dysbiosis Crohn’s disease (CD) patients often an expansion Escherichia coli . Here, we investigated ability such E. isolates to avoid neutrophil activation utilize reactive oxygen species. Neutrophil was detected vitro normal human blood via luminol chemiluminescence (CL)...
Aim of the study. Neutrophil extracellular traps (NETs) have been reported to participate in many pathological processes, which points at NET concentration blood as an important indicator body condition. When studying NETs, their is usually evaluated indirectly, has a number shortcomings. We aimed measure directly amount NETs integral structures, using smears most suitable tool for clinical practice, and carry out measurements levels type 2 diabetes mellitus (T2DM) patients with necrotic...
In cases of any acute surgical abdominal disease the progression purulent inflammation can lead to local or diffuse peritonitis. The indicators degree and specificity inflammatory response in blood such as cytokine concentration, neutrophil activity, plasma antioxidant capacity (thiols concentration) could be considered potential predictors complications. luminol-dependent chemiluminescence (CL) activated by phorbol ester (PMA), concentration cytokines IL-6, IL-8, IL-10, myeloperoxidase...