A5090321881- Enzyme Structure and Function
- Heat shock proteins research
- Advanced Electron Microscopy Techniques and Applications
- Protein Structure and Dynamics
- RNA and protein synthesis mechanisms
- Glycosylation and Glycoproteins Research
- RNA modifications and cancer
- Enzyme Production and Characterization
- Bacteriophages and microbial interactions
- Viral gastroenteritis research and epidemiology
- Prion Diseases and Protein Misfolding
- Mosquito-borne diseases and control
- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Vectors
- Biochemical and Molecular Research
- Cytomegalovirus and herpesvirus research
- Influenza Virus Research Studies
- Herpesvirus Infections and Treatments
- Radiopharmaceutical Chemistry and Applications
- Monoclonal and Polyclonal Antibodies Research
- Hepatitis B Virus Studies
- Photosynthetic Processes and Mechanisms
- Vector-borne infectious diseases
- Advanced X-ray Imaging Techniques
- Escherichia coli research studies
Kurchatov Institute
2018-2025
Petersburg Nuclear Physics Institute
2019-2024
Federal Scientific Research Centre Crystallography and Photonics
2019-2024
Russian Academy of Sciences
2020-2024
A.V. Shubnikov Institute of Crystallography
2020
Tick-borne encephalitis virus (TBEV) causes a severe disease, tick-borne (TBE), that has substantial epidemiological importance for Northern Eurasia. Between 10,000 and 15,000 TBE cases are registered annually despite the availability of effective formaldehyde-inactivated full-virion vaccines due to insufficient vaccination coverage, as well sporadic vaccine breakthrough. The development improved would benefit from atomic resolution structure antigen. Here we report refined single-particle...
X-ray imaging of virus particles at the European XFEL could eventually allow their complete structures to be solved, potentially approaching resolution other structural virology methods. To achieve this ambitious goal with today's technologies, about 1 ml purified suspension containing least 10
Macrolides are one of the most successful and widely used classes antibacterials, which kill or stop growth pathogenic bacteria by binding near active site ribosome interfering with protein synthesis. Dirithromycin is a derivative prototype macrolide erythromycin additional hydrophobic side chain. In our recent study, we have discovered that chain dirithromycin forms lone pair-π stacking interaction aromatic imidazole ring His69 residue in ribosomal uL4 Thermus thermophilus 70S ribosome....
Abstract The GroEL–GroES chaperonin complex is a bacterial protein folding system, functioning in an ATP-dependent manner. Upon ATP binding and hydrolysis, it undergoes multiple stages linked to substrate binding, release. Structural methods helped reveal several conformational states provide more information about the functional cycle. Here, using cryo-EM we resolved two nucleotide-bound structures of bullet-shaped 1 at 3.4 Å resolution. main difference between them relative orientation...
Abstract Translation is a critical step in gene expression and target for multiple antibiotics. Inhibition of protein synthesis by many antibiotics depends on mRNA context, making them selective towards particular mRNAs. Toe-printing standard low-throughput method to determine ribosome position along mRNA. Hereby we report the development application Toe-seq method, combining toe-printing with next-generation sequencing. proved be efficient monitor translation its inhibition library over 37...
Study of mechanisms by which antibodies recognize different viral strains is necessary for the development new drugs and vaccines to treat COVID-19 other infections. Here, we report 2.5 Å cryo-EM structure SARS-CoV-2 Delta trimeric S-protein in complex with Fab recombinant analog REGN10987 neutralizing antibody. adopts "two RBD-down one RBD-up" conformation. interacts RBDs both conformations, blocking recognition angiotensin converting enzyme-2. Three-dimensional variability analysis reveals...
Nowadays, polypropylene-based nonwovens are used in many areas, from filtration to medicine. One of the methods for obtaining such materials is melt electrospinning. In some cases, it especially interesting produce composite fibers with a high degree filling. this work, influence filling isotactic polypropylene calcium carbonate on structure and properties nonwoven obtained by electrospinning was studied. It shown that possible, even at filler content 50%, while average diameter increases...
The molecular chaperone GroEL is designed to promote protein folding and prevent aggregation. However, the interaction between prion protein, PrPC, could lead pathogenic transformation of latter aggregation-prone PrPSc form. Here, basis interactions in GroEL–PrP complex studied with cryo-EM dynamics approaches. obtained structure shows PrP be bound several subunits at level their apical domains. According MD simulations, disordered N-domain forms much more intermolecular contacts GroEL. Upon...
The Dps protein of Escherichia coli is a homododecamer with an internal cavity accumulating iron oxides, transformed from ambient toxic Fe2+ into harmless inorganic core. High resolution cryogenic transmission electron microscopy was applied to visualize the molecules and characterize their ability self-organization. Due ultrafast freezing, dodecameric particles were observed as "snapshot" natural state, highly ordered two-dimensional layers hexagonal symmetry obtained. Thus, it became...
The most extensively studied β-d-galactosidases (EC3.2.1.23) belonging to four glycoside hydrolase (GH) families 1, 2, 35, and 42 are widely distributed among Bacteria, Archaea Eukaryotes. Here, we report a novel GH35 family β-galactosidase from the hyperthermophilic Thermoprotei archaeon Desulfurococcus amylolyticus (DaβGal). Unlike fungal monomeric six-domain β-galactosidases, DaβGal enzyme is dimer; it has an extra jelly roll domain D7 three composite domains (D4, D5, D6) that formed by...
In this paper we present an approach to the on-the-fly Cryo-EM data acquisition and analysis using High Performance Data Analysis concept. An of bottlenecks in currently used processing is presented, new iterative scheme for near real time implementation discussed. The proposed that will considerably reduce required protein (or other biological objects, e.g. viruses) 3D structure determination allow eliminating manual interventions processing.
The structure of cytochrome c nitrite reductase from the bacterium Thioalkalivibrio nitratireducens was determined by cryo-electron microscopy (cryo-EM) at a 2.56 resolution. Possible structural heterogeneity enzyme assessed. backbone and side-chain orientations in cryo-EM-based model are, general, similar to those high-resolution X-ray diffraction this enzyme.