A5063708588- Blood properties and coagulation
- Erythrocyte Function and Pathophysiology
- Neurological Disorders and Treatments
- Microfluidic and Bio-sensing Technologies
- Spectroscopy Techniques in Biomedical and Chemical Research
- Antioxidant Activity and Oxidative Stress
- Lipid Membrane Structure and Behavior
- Proteoglycans and glycosaminoglycans research
- Algal biology and biofuel production
- Chemical synthesis and pharmacological studies
- Advanced Scientific Research Methods
- Orbital Angular Momentum in Optics
- Heparin-Induced Thrombocytopenia and Thrombosis
- Enzyme Structure and Function
- Protein Structure and Dynamics
- Heat shock proteins research
- Rheology and Fluid Dynamics Studies
- Protein Interaction Studies and Fluorescence Analysis
- Photosynthetic Processes and Mechanisms
- Photoreceptor and optogenetics research
- Blood groups and transfusion
- Retinal Diseases and Treatments
- Advanced Fluorescence Microscopy Techniques
- Platelet Disorders and Treatments
- Porphyrin and Phthalocyanine Chemistry
Lomonosov Moscow State University
2007-2025
Saarland University
2024-2025
Moscow State University
2021-2023
Ministry of Defence of the Russian Federation
2020
National Research Nuclear University MEPhI
2015
National Research Tomsk State University
2015
Tomsk Polytechnic University
2015
Kurchatov Institute
2013-2015
Tver State University
2007-2014
Research Institute of Synthetic Fiber with the Pilot Plant
2013
Blood cell analysis is one of the standard clinical tests.Despite widespread use exogenous markers for blood quantification, label-free optical methods are still high demand due to their possibility in vivo application and signal specific biochemical state provided by native fluorophores.Here we report results characterization using fluorescence imaging techniques flow-cytometry.Autofluorescence parameters different types -white cells, red erythrophagocytic cells -are assessed analyzed terms...
Enveloped viruses, such as flaviviruses and coronaviruses, are pathogens of significant medical concern that cause severe infections in humans. Some photosensitizers known to possess virucidal activity against enveloped targeting their lipid bilayer. Here we report a series halogenated difluoroboron-dipyrromethene (BODIPYs) with strong virus-inactivating activity. Our structure–activity relationship analysis revealed BODIPY scaffolds heavy halogen atom demonstrate efficacy both tick-borne...
Endogenous autofluorescence of biological tissues is an important source information for biomedical diagnostics. Despite the molecular complexity tissues, list commonly known fluorophores strictly limited. Still, question sources red and near-infrared excited remains open. In this work we demonstrated that oxidation products organic components (lipids, proteins, amino acids, etc.) can serve as such autofluorescence. Using model solutions cell systems (human keratinocytes) under oxidative...
Lipofuscin of retinal pigment epithelium (RPE) cells is a complex heterogeneous system chromophores which accumulates as granules during the cell’s lifespan. serves source various cytotoxic effects linked with oxidative stress. Several age-related eye diseases such macular degeneration retina, well some severe inherited pathologies, are accompanied by significant increase in lipofuscin granule concentration. The accumulation carotenoids RPE could provide an effective antioxidant protection...
An elevated concentration of fibrinogen in blood is a significant risk factor during many pathological diseases, as it leads to an increase red cells (RBC) aggregation, resulting hemorheological disorders. Despite the biomedical importance, mechanisms fibrinogen-induced RBC aggregation are still debatable. One discussed models non-specific adsorption macromolecules onto membrane, leading bridging aggregates. However, recent works point specific character interaction between and membrane....
Signaling pathways of red blood cells (RBC) micromechanics regulation, which are responsible for maintaining the microcirculation, constitute an important property RBC physiology. Selective control over these processes may serve indispensable tool correction hemorheological disorders, accompany a number systemic diseases (diabetes mellitus I&II, arterial hypertension, malaria, etc.). Namely, activation certain involving adenylyl cyclase provide fast adaptive regulation deformability (RBC-D)....
This study aims to highlight the problems with implementing optical techniques (laser tweezers, diffuse light scattering and laser diffractometry) in clinical hemorheological practice. We provide feasibility of these assess microrheological effects various molecular mechanisms affecting RBC aggregation deformability. In particular, we show that they allow assessment changes whole blood samples both on level single cells large ensembles cells. Application methods allows for studying because...
Abstract We consider the effect of a tightly focused laser beam with wavelength 1064 nm and power from 10 to 160 mW on red blood cells during their optical trapping tweezers. It is found that shape cell, which alters after trapping, ceases change when duration less than 5 min 60 mW. At above 80 mW, cell begins fold at about 1 min, powers 100 – 150 membrane ruptures in 3 trapping. also repeated short-term capture an trap, deformation properties change: it becomes more rigid. The obtained...
Carotenoids are potent antioxidants with a wide range of biomedical applications. However, their delivery into human cells is challenging and relatively inefficient. While the use natural water-soluble carotenoproteins capable to reversibly bind carotenoids transfer them membranes promising, quantitative estimation remains unclear. In present work, we studied echinenone (ECN) by cyanobacterial carotenoprotein AnaCTDH (C-terminal domain homolog Orange Carotenoid Protein from Anabaena),...
The aggregation of red blood cells (RBCs) is a complex phenomenon that strongly impacts flow and tissue perfusion. Despite extensive research for more than 50 years, physical mechanisms govern RBC are still under debate. Two proposed based on bridging depletion interactions between RBCs due to the presence macromolecules in plasma. hypothesis assumes formation bonds through adsorbing macromolecules, while mechanism results from exclusion inter-cellular space, leading effective attraction....
Photodamage to the outer segments of photoreceptor cells and their impaired utilization by retinal pigment epithelium (RPE) contribute development age‐related macular degeneration (AMD) leading blindness. Degeneration RPE is triggered reactive oxygen species (ROS) produced photochemical reactions involving bisretinoids, by‐products visual cycle, which accumulate in discs lipofuscin granules RPE. Carotenoids, natural antioxidants with high potential efficacy against a wide range ROS, may...
Two-channel laser tweezers are used to measure the interaction kinetics of two erythrocytes at initial stage aggregation, i.e., formation a pair aggregate in vitro. The study is important both for understanding fundamental aggregation mechanisms and evaluating differences dynamics depending on presence or absence diseases that disturb blood flow parameters and, therefore, oxygen supply tissues. We analyse samples from more than 60 patients with arterial hypertension, as well group healthy...
Red blood cells (RBCs) are able to interact and communicate with endothelial (ECs). Under some pathological or even normal conditions, the adhesion of RBCs endothelium can be observed. Presently, mechanisms many aspects interaction between ECs not fully understood. In this work, we considered single in vitro aiming quantitatively determine force using laser tweezers. Measurements were performed under different concentrations proaggregant macromolecules presence absence tumor necrosis factor...
Molybdenum nanocrystalline powder particles using electric blasting circuit method after molybdenum destruction were formed. During this process the separate molybdenum-containing as products of explosion reaction with each other and getting thinner during cooling stage combining. Their surface is partially oxygenated in argon (Ar) air addition atmosphere. When finished size distribution had three-modal profile, most 120 nm diameter according to electronic microscope data. Oxygenated...
Microrheological parameters of red blood cells (RBCs) and viscosity were studied by different optical rheometric techniques. Measurements performed under in vitro condition both on the EDTA-stabilized whole samples at various temperatures RBCs autologous serum without platelets concentrations proaggregant macromolecules (Dextran 150, Dextran 500 albumin). The nonlinear dependences aggregation concentration observed. Rheometry human demonstrates a power law dependence suspension temperature...