A5026464636- Microtubule and mitosis dynamics
- Protist diversity and phylogeny
- Genetic and Kidney Cyst Diseases
- Photosynthetic Processes and Mechanisms
- Genetics, Aging, and Longevity in Model Organisms
- Advanced Fluorescence Microscopy Techniques
- RNA and protein synthesis mechanisms
- Photoreceptor and optogenetics research
- RNA Research and Splicing
- RNA modifications and cancer
- GDF15 and Related Biomarkers
- RNA regulation and disease
- Mitochondrial Function and Pathology
- Resilience and Mental Health
Vrije Universiteit Amsterdam
2020-2024
Lomonosov Moscow State University
2019
Genes coding for small peptides have been frequently misannotated as long noncoding RNA (lncRNA) genes. Here we demonstrated that one such transcript is translated into a 56-amino-acid-long peptide conserved in chordates, corroborating the work published while this manuscript was under review. The Mtln could be detected mitochondria of mouse cell lines and tissues. In line with its mitochondrial localization, lack decreases activity respiratory chain complex I. Unlike integral components...
Abstract Intraflagellar transport (IFT) orchestrates entry of proteins into primary cilia. At the ciliary base, assembled IFT trains, driven by kinesin-2 motors, can cargo cilium, across crowded transition zone. How trains assemble at base and how associate with them is far from understood. Here, we use single-molecule imaging in cilia C. elegans chemosensory neurons to directly visualize kinesin-II OSM-3, as well anterograde proteins, dynein tubulin. Single-particle tracking shows that...
Intracellular transport of organelles and biomolecules is vital for several cellular processes. Single-molecule fluorescence microscopy can illuminate molecular aspects the dynamics individual that remain unresolved in ensemble experiments. For example, studying single-molecule trajectories moving reveal motility properties such as velocity, diffusivity, location duration pauses, etc. We use imaging to study microtubule-based motor proteins their cargo primary cilia living C. elegans. To...
RNA interference (RNAi)-based therapeutics hold the potential for dominant genetic disorders, enabling sequence-specific inhibition of pathogenic gene products. We aimed to direct RNAi selective suppression heterozygous
Single-particle imaging using laser-illuminated widefield epi-fluorescence microscopy is a powerful tool to investigate molecular processes in vivo. Performing high-quality single-molecule such biological systems, however, remains challenge due difficulties controlling the number of fluorescing molecules, photobleaching, and autofluorescence background. Here, we show that by exciting only small, 5-15 μm wide region chemosensory neurons live C. elegans, can significantly improve duration...
Summary Intraflagellar transport (IFT) orchestrates entry of proteins into primary cilia. At the ciliary base, assembled IFT trains, driven by kinesin-2 motors, can cargo cilium, across crowded transition zone (TZ). How trains assemble at base and how associate with them is far from understood. Here, we use single-molecule imaging in cilia C. elegans chemosensory neurons to directly visualize motors kinesin-II OSM-3, as well anterograde dynein tubulin. Single-particle tracking shows that...
Primary cilia, organelles protruding from the surface of eukaryotic cells, act as cellular antennae to detect and transmit signals extracellular environment. They are built maintained by continuous cycles intraflagellar transport (IFT), where ciliary proteins transported between base tip. These originate cell body because cilia lack protein synthesis machinery. How input affects IFT function is not well understood. Here, we use femtosecond-laser ablation perturb dendritic chemosensory in...
Abstract Single-particle imaging using laser-illuminated widefield epi-fluorescence microscopy is a powerful tool to investigate molecular processes in vivo . Performing high-quality single-molecule such biological systems, however, remains challenge due difficulties controlling the number of fluorescing molecules, photobleaching, and autofluorescence background. Here, we show that by exciting only small, 5-15 µm wide region chemosensory neurons live C. elegans , can significantly improve...