Rapid sensory adaptation is observed across all systems, and strongly shapes percepts in complex environments. Yet despite its ubiquity likely necessity for survival, the mechanistic basis poorly understood. A wide range of primarily vitro anesthetized studies have demonstrated emergence at level primary cortex, with only modest signatures earlier stages processing. The nature rapid how it representations during wakefulness, thus potential role perceptual adaptation, underexplored, as are...

With the recent breakthrough in genetically expressed voltage indicators (GEVIs), there has been a tremendous demand to determine capabilities of these sensors vivo. Novel sensitive fluorescent proteins allow for direct measurement neuron membrane potential changes through fluorescence. Here, we utilized ArcLight, recently developed GEVI, and examined functional characteristics widely used mouse somatosensory whisker pathway. We measured resulting evoked fluorescence using wide-field...

Abstract Behavioral experience and flexibility are crucial for survival in a constantly changing environment. Despite evolutionary pressures to develop adaptive behavioral strategies dynamically sensory landscape, the underlying neural correlates have not been well explored. Here, we use genetically encoded voltage imaging measure signals primary somatosensory cortex (S1) during learning adaptation mouse. In response stimulus statistics, mice adopt strategy that modifies their detection...

Even in the absence of specific sensory input or a behavioral task, brain produces structured patterns activity. This organized activity is modulated by changes arousal. Here, we use wide-field voltage imaging to establish how arousal relates cortical network and hemodynamic spontaneously behaving head-fixed male female mice expressing voltage-sensitive fluorescent FRET sensor Butterfly 1.2. We find that global signals are both positively correlated with maximum correlation 0.5 0.25,...

Behavioral adaptation is a prerequisite for survival in constantly changing sensory environment, but the underlying strategies and relevant variables driving adaptive behavior are not well understood. Many learning models neural theories consider probabilistic computations as an efficient way to solve variety of tasks, especially if uncertainty involved. Although this suggests possible role inference expectation behaviors, there little any evidence relationship experimentally. Here, we...

1.1.1 Abstract Whole-cell patch-clamp recording in vivo is the gold-standard method for measuring subthreshold electrophysiology from single cells during behavioural tasks, sensory stimulations, and optogenetic manipulation. However, these recordings require a tight, gigaohm resistance, seal between glass pipette electrode’s aperture cell’s membrane. These seals are difficult to form, especially vivo, part because of strong dependence on distance cell We elucidate utilize this dependency...

Abstract Rapid sensory adaptation is observed across all systems, and strongly shapes percepts in complex environments. Yet despite its ubiquity likely necessity for survival, the mechanistic basis poorly understood. A wide range of primarily in-vitro anesthetized studies have demonstrated emergence at level primary cortex, with only modest signatures earlier stages processing. The nature rapid how it representations during wakefulness, thus potential role perceptual adaptation,...

Summary The thalamus controls transmission of sensory signals from periphery to cortex, ultimately shaping perception. Despite this significant role, dynamic thalamic gating and the consequences for downstream cortical representations have not been well studied in awake brain. We optogenetically modulated ventro-posterior medial vibrissa pathway mouse, measured spiking activity thalamus, primary somatosensory cortex (S1) using extracellular electrophysiology genetically encoded voltage...

ABSTRACT Behavioral experience and flexibility are crucial for survival in a constantly changing environment. Despite evolutionary pressures to develop adaptive behavioral strategies dynamically sensory landscape, the underlying neural correlates have not been well explored. Here, we use genetically encoded voltage imaging measure signals primary somatosensory cortex (S1) during learning adaptation mouse. In response stimulus statistics, mice adopt strategy that modifies their detection...

Abstract Models of basal ganglia function predict that tonic inhibitory output to motor thalamus suppresses unwanted movements, and a decrease in such activity leads action selection. A direct test these outcomes thalamic inhibition has not been performed, however. To conduct test, we utilized rapid optogenetic activation inactivation the GABAergic substantia nigra pars reticulata (SNr) mice were trained sensory cued left/right licking task. Directional tasks have previously shown depend on...

Abstract Even in the absence of specific sensory input or a behavioral task, brain produces structured patterns activity. This organized activity is modulated by changes arousal. Here, we use wide-field voltage imaging to establish how arousal relates cortical network and hemodynamic spontaneously behaving head-fixed male female mice expressing voltage-sensitive fluorescent FRET sensor Butterfly 1.2. We find that global signals are both positively correlated with maximum correlation 0.5 0.25...