We propose a serial time-division-multiplexing sensor network based on ultraweak fiber Bragg gratings (FBGs). Twelve FBGs are distinguished with spatial resolution of about 0.2 m, and their spectra resolved an accuracy 10 pm. The low crosstalk the makes it possible to multiplex over 1000 along single optical fiber.

We demonstrated a wavelength scanning time division multiplexing of 1000 ultra-weak fiber Bragg gratings (FBG) for distributed temperature sensing. The strong capability and low crosstalk the FBG sensors is investigated through both theoretical analysis experiment. An automated fabrication system was developed fast 1000-FBG fabrication. measurement accuracy wavelengths more than 80% FBGs less 20 pm, corresponding to 2 <formula formulatype="inline"...

Microglia transform in response to changes sensory or neural activity, such as deprivation. However, little is known about how specific frequencies of brain rhythms, affect microglia and cytokine signaling. Using visual noninvasive flickering stimulation (flicker) induce electrical activity at 40 hertz, within the gamma band, 20 beta we found that these rhythms differentially microglial morphology expression healthy animals. Flicker induced certain cytokines independently microglia,...

A fully disentangled variational auto-encoder (VAE) aims to identify latent components from observations. However, enforcing full independence between all may be too strict for certain datasets. In some cases, multiple factors entangled together in a non-separable manner, or single independent semantic meaning could represented by within higher-dimensional manifold. To address such scenarios with greater flexibility, we develop the Partially Disentangled VAE (PDisVAE), which generalizes...

Abstract Widefield imaging with genetically encoded voltage indicators (GEVIs) is a promising approach for understanding the role of large cortical networks in neural coding behavior. However, limited performance current GEVIs restricts their deployment single-trial rapid neuronal dynamics. Here, we developed high-throughput platform to screen that combine fast kinetics high brightness, sensitivity, and photostability under widefield one-photon illumination. Rounds directed evolution...

A high-speed quasi-distributed fiber optic sensing technique is proposed. link consisting multiple identical Bragg gratings (FBGs) was interrogated by a pulsed light source, and dispersive unit at the receiver end used to convert wavelength shifts of reflected pulses into time-delay changes. This wavelength-dependent time delay can be accurately calculated fast cross-correlation-based algorithm, enabling distributed sensing. experimentally demonstrated using containing 2014 FBGs with...

Even in the absence of specific sensory input or a behavioral task, brain produces structured patterns activity. This organized activity is modulated by changes arousal. Here, we use wide-field voltage imaging to establish how arousal relates cortical network and hemodynamic spontaneously behaving head-fixed male female mice expressing voltage-sensitive fluorescent FRET sensor Butterfly 1.2. We find that global signals are both positively correlated with maximum correlation 0.5 0.25,...

Fully distributed fiber-optic biological sensing is demonstrated based on a traveling long-period grating (LPG) in single-mode fiber coated with functional film. The temporal LPG generated by an acoustic pulse propagating along the fiber. Immunoglobulin G (IgG) immobilized onto surface of via ionic self-assembly. We show that specific antigen-antibody binding can introduce measurable shift transmission optical spectrum when it passes through 10-cm pretreated segment. It also shown...

A highly sensitive fully distributed fiber-optic temperature sensing technique is proposed and demonstrated based on a transient traveling rocking grating. The grating generated by pulsed acoustic torsional waves propagating along the fiber. measured sensitivity 1000 ppm/°C experimentally mainly due to dependence of fiber birefringence. This may also serve other fully-distributed applications using specially designed fibers.

A potential technique for fully distributed fiber-optic hydrogen sensing is demonstrated based on a traveling long-period grating (LPG) in single-mode fiber coated with platinum (Pt) catalyst layer. The LPG generated by an acoustic pulse propagating along the fiber. Pt-coated section heated thermal energy released from Pt-assisted combustion of H <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> and O . resulted temperature change gives...

A fiber-optic sensing platform based on a transient and traveling long-period grating (LPG) in single-mode optical fiber has been proposed demonstrated. The LPG is generated by pulsed acoustic waves that propagate along the fiber. First, we demonstrate for temperature measurement By coating with ultrathin ionically self-assembled multilayers, then show capable of detecting nanometer thickness variations compensation method also Because acoustically travels fiber, this advance expected to...

The application of photonic biosensor assays to diagnose the category-A select agent Francisella tularensis was investigated. Both interferometric and long period fiber grating sensing structures were successfully demonstrated; both these sensors are capable detecting optical changes induced by either immunological binding or DNA hybridization. Detection made possible attachment probes immunoglobulins (IgG) directly surface via layer-by-layer electrostatic self-assembly. An tested using a...

Abstract Widefield imaging with genetically encoded voltage indicators (GEVIs) is a promising approach for understanding the role of large cortical networks in neural coding behavior. However, slow kinetics current GEVIs limit their deployment single-trial rapid neuronal dynamics. Here, we developed high-throughput platform to screen that combine fast high brightness, sensitivity, and photostability under widefield one-photon illumination. Rounds directed evolution produced JEDI-1P,...

This paper presents a new label-free DNA optical sensor based on the microgap structured Fabry-Perot interferometer. An cavity is formed by sealed between singlemode fiber and well-cleaved end. The capture immobilized end layer-by-layer electrostatic self-assembly. detects hybridization of target with complementary observing thickness variation. Experimental results demonstrate that this has sensitivity 6 nmol/ml can identify 2-bp mismatch.

Widefield optical imaging allows for novel methods of network-level functional connectivity analysis neuronal voltage and hemodynamic signals to aid the interpretation metabolic activation across mouse cortex.

Through-skull wide-field imaging in awake mice was used to compare functional connectivity neuronal voltage signals across different frequencies those obtained from a red reference fluorophore track optical fluctuations due hemodynamics.

A temperature sensor link based on wavelength-multiplexed fiber Bragg grating (FBG) was designed and fabricated for distributed measurement in a jet engine nozzle under field conditions. Eight FBGs with different wavelengths ranging from 1520 nm to 1560 were along one single-mode which packaged inside stainless steel tube. The reflected signal the simultaneously collected by an optical sensing interrogator converted into information. tube embedded flange assembly attached engine. Three...

Abstract Microglia, the brain’s primary immune cells, transform in response to changes sensory or neural activity, like deprivation. However, little is known about how specific frequencies of brain rhythms, impact microglia and cytokine signaling. Using visual noninvasive flickering stimulation (flicker) induce electrical activity at different frequencies, 40Hz, within gamma band 20Hz, beta band, we discovered these rhythms differentially affect microglial morphology expression healthy...

A method for equalizing the path lengths of two arms an optical fiber coupler is presented as a critical step towards construction high-resolution 3-D interferometric imaging system. Based on white-light interferometry (WLI), technique combines absolute measurement capability with ability to accurately measure near-zero length differences. controlled temperature increase in one arm generates shift WLI measurement, allowing fibers be polished near-equal lengths. The demonstrated equalize...

This paper gives a review of proposed fully-distributed fiber-optic sensing technique based on traveling long-period grating (LPG) in single-mode optical fiber. The LPG is generated by pulsed acoustic waves that propagate along the Based this platform, first we demonstrated temperature measurement 2.5m Then coating fiber with functional coatings, biological and chemical sensing. In experiment, immunoglobulin G (IgG) was immobilized onto surface, showed only specific antigen-antibody binding...

The large multiplexing number of FBGs exposes a requirement for the effective and repeatable fabrication method. In this paper we report development an automatic FBG system, which meets mass production. There are four major functional parts in system: fiber feeding CO₂ laser coating removal writing system collecting system. uses motors gears to accurately move optical where will be made. is based on heat effect laser, decompose evaporate selected fiber. UV photosensitivity A...