A5042452743- Neuroscience and Neural Engineering
- Neural dynamics and brain function
- Neuroscience and Neuropharmacology Research
- Advanced Memory and Neural Computing
- Photoreceptor and optogenetics research
- EEG and Brain-Computer Interfaces
- Receptor Mechanisms and Signaling
- Polymer Surface Interaction Studies
- 3D Printing in Biomedical Research
- Acoustic Wave Phenomena Research
- Nanowire Synthesis and Applications
- Surface Modification and Superhydrophobicity
- Shape Memory Alloy Transformations
- Electrospun Nanofibers in Biomedical Applications
- Electrochemical Analysis and Applications
- Hydrogels: synthesis, properties, applications
- Additive Manufacturing and 3D Printing Technologies
- Nicotinic Acetylcholine Receptors Study
- Molecular Junctions and Nanostructures
- Soft Robotics and Applications
- Cell Image Analysis Techniques
- Speech and Audio Processing
- Analytical Chemistry and Sensors
- Metamaterials and Metasurfaces Applications
- Electrochemical sensors and biosensors
Georgia Institute of Technology
2019-2024
National Eye Institute
2024
Janelia Research Campus
2024
Emory University
2021-2024
National Institutes of Health
2024
Massachusetts Institute of Technology
2024
McGovern Institute for Brain Research
2024
Howard Hughes Medical Institute
2024
Columbia University
2024
Presbyterian Hospital
2017-2023
Potentiometric sensing of ions with ion-selective electrodes (ISEs) is a powerful technique for selective and sensitive measurement in complex matrices. The application ISEs generally limited to laboratory settings, because most commercially available reference are large, delicate, expensive, not suitable point-of-use or point-of-care measurements. This work utilizes cotton thread as substrate fabrication robust miniaturized that applications. Thread-based Cl-, K+, Na+, Ca2+ were developed....
Intracellular patch-clamp electrophysiology, one of the most ubiquitous, high-fidelity techniques in biophysics, remains laborious and low-throughput. While previous efforts have succeeded at automating some steps technique, here we demonstrate a robotic 'PatcherBot' system that can perform many recordings sequentially, fully unattended.Comprehensive automation is accomplished by outfitting robot with machine vision, cleaning pipettes instead manually exchanging them.the PatcherBot obtain...
A common electrophysiology technique used in neuroscience is patch clamp: a method which glass pipette electrode facilitates single cell electrical recordings from neurons. Typically, clamp done manually an electrophysiologist views brain slice under microscope, visually selects neuron to patch, and moves the into close proximity break through seal its membrane. While recent advances field of clamping have enabled partial automation, task detecting healthy neuronal soma acute tissue slices...
This paper reports the rapid 3D printing of tough (toughness, <italic>U</italic><sub>T</sub>, up to 141.6 kJ m<sup>−3</sup>), highly solvated (<italic>ϕ</italic><sup>water</sup> ∼ 60 v/o), and antifouling hybrid hydrogels for potential uses in biomedical, smart materials, sensor applications, using a zwitterionic photochemistry compatible with stereolithography (SLA).
Active acoustic metamaterials incorporate electric circuit elements that input energy into an otherwise passive medium to aptly modulate the effective material properties. Here, we propose active metamaterial with Willis coupling drastically extend tunability of density and bulk modulus accessible parameter range enlarged by at least two orders magnitude compared a non-Willis metamaterial. Traditional designs are based on local resonances without considering limit their range. Our design...
Communication between neuronal cells, which is central to brain function, performed by several classes of ligand-gated ionotropic receptors. The gold-standard technique for measuring rapid receptor response agonist manual patch-clamp electrophysiology, capable the highest temporal resolution any current electrophysiology technique. We report an automated high-precision system that substantially improves throughput these time-consuming pharmacological experiments. patcherBotPharma enables...
Summary Significant technical challenges exist when measuring synaptic connections between neurons in living brain tissue. The patch clamping technique, used to probe for connections, is manually laborious and time-consuming. To improve its efficiency, we pursued another approach: instead of retracting all electrodes after each recording attempt, cleaned just one them reused it obtain while maintaining the others. With new clamp many can be probed. By placing pipette front others this way,...
The whole-cell patch-clamp method is a gold standard for single-cell analysis of electrical activity, cellular morphology, and gene expression. Prior to our discovery that pipettes could be cleaned reused, experimental throughput automation were limited by the need replace manually after each experiment. This article presents an optimized protocol pipette cleaning, which enables it performed quickly (< 30 s), resulting in high yield recording success rate (> 90%) over 100 reuses single...
Abstract This paper describes the methodology for rapid prototyping of nitinol structures by heat setting. Nitinol is a shape memory alloy commonly used in implantable medical devices. The proposed technique, based on 3D printing, can be to effectively iterate multiple designs different types We describe and low-cost process ceramic replica molding standard printed parts create high-temperature resistant fixtures, suitable setting nitinol. technique represents low cost (&lt;$20 materials...
Patch clamp electrophysiology is a common technique used in neuroscience to understand individual neuron behavior, allowing one record current and voltage changes with superior spatiotemporal resolution compared most methods. While patch experiments produce high fidelity data, the onerous labor intensive. Despite emergence of systems that automate key stages typical procedure, full automation remains elusive. pipettes can miss target cell during automated because positioning errors robotic...
Abstract The ability to optically image cellular transmembrane voltage at millisecond-timescale resolution can offer unprecedented insight into the function of living brains in behaving animals. chemigenetic indicator Voltron is bright and photostable, making it a favorable choice for long vivo imaging neuronal populations resolution. Improving sensitivity would allow better detection spiking subthreshold signals. We performed site saturation mutagenesis 40 positions screened increased ΔF/F...
Significant technical challenges exist when measuring synaptic connections between neurons in living brain tissue. The patch clamping technique, used to probe for connections, is manually laborious and time-consuming. To improve its efficiency, we pursued another approach: instead of retracting all electrodes after each recording attempt, cleaned just one them reused it obtain while maintaining the others. With new clamp many can be probed. By placing pipette front others this way, ‘walk’...
Significant technical challenges exist when measuring synaptic connections between neurons in living brain tissue. The patch clamping technique, used to probe for connections, is manually laborious and time-consuming. To improve its efficiency, we pursued another approach: instead of retracting all electrodes after each recording attempt, cleaned just one them reused it obtain while maintaining the others. With new clamp many can be probed. By placing pipette front others this way, “walk”...
Significant technical challenges exist when measuring synaptic connections between neurons in living brain tissue. The patch clamping technique, used to probe for connections, is manually laborious and time-consuming. To improve its efficiency, we pursued another approach: instead of retracting all electrodes after each recording attempt, cleaned just one them reused it obtain while maintaining the others. With new clamp many can be probed. By placing pipette front others this way, “walk”...
Significant technical challenges exist when measuring synaptic connections between neurons in living brain tissue. The patch clamping technique, used to probe for connections, is manually laborious and time-consuming. To improve its efficiency, we pursued another approach: instead of retracting all electrodes after each recording attempt, cleaned just one them reused it obtain while maintaining the others. With new clamp many can be probed. By placing pipette front others this way, ‘walk’...
Preventing occurrence of thrombosis and biofouling is essential for safety efficacy an intravascular device.The slippery liquid-infused porous surface (SLIPS) technology has promise to achieve this goal by forming a liquidrepellent layer on the blood contacting materials.As adhesion biomolecules greatly inhibited, SLIPS-treated reduces thrombosing biofouling.This study demonstrates that in static conditions, cells can adhere surfaces without significant decreases attachment or migration, as...
Abstract Serial section electron microscopy (ssEM), a technique where volumes of tissue can be anatomically reconstructed by imaging consecutive slices, has proven to powerful tool for the investigation brain anatomy. Between process cutting or “sections,” and them, however, handling 10°−10 6 delicate sections remains bottleneck in ssEM, especially batches “mesoscale” regime, i.e., 10 2 –10 3 sections. We present device that transports positions sections, accurately repeatability, automated,...