A5030170409- Advanced Fluorescence Microscopy Techniques
- Photoreceptor and optogenetics research
- Optical Coherence Tomography Applications
- Photoacoustic and Ultrasonic Imaging
- Neuroscience and Neural Engineering
- Silicon and Solar Cell Technologies
- Corneal surgery and disorders
- Optical Imaging and Spectroscopy Techniques
- Semiconductor materials and devices
- Neural dynamics and brain function
- Glaucoma and retinal disorders
- Cell Image Analysis Techniques
- Ocular Infections and Treatments
- Semiconductor materials and interfaces
- Photovoltaic Systems and Sustainability
- Photovoltaic System Optimization Techniques
- Digital Holography and Microscopy
- Cerebral Venous Sinus Thrombosis
- Retinal Imaging and Analysis
- Retinal and Optic Conditions
- Random lasers and scattering media
- Quantum Chromodynamics and Particle Interactions
- Electrical Fault Detection and Protection
- Digital Imaging for Blood Diseases
- Neural Networks and Reservoir Computing
Massachusetts Institute of Technology
2024-2025
Boston University
2017-2023
Rider University
2010
University of Tampa
2010
The last decade has seen the development of a wide set tools, such as wavefront shaping, computational or fundamental methods, that allow to understand and control light propagation in complex medium, biological tissues multimode fibers. A vibrant diverse community is now working on this field, revolutionized prospect diffraction-limited imaging at depth tissues. This roadmap highlights several key aspects fast developing some challenges opportunities ahead.
Aim Over the past several decades, optical sectioning technologies have emerged as valuable tools for evaluating tissue histology. Unlike conventional sectioning, these allow real-time intraoperative assessments and more efficient triage. In era of digital pathology, demand high-quality, high-throughput platforms is increasing, they eliminate need traditional slide preparation scanning, potentially transforming anatomical pathology workflows. While non-linear microscopy (NLM) has...
The inherent constraints on resolution, speed and field of view have hindered the development high-speed, three-dimensional microscopy techniques over large scales. Here, we present a multiplane line-scan imaging strategy, which uses series axially distributed reflecting slits to probe different depths within sample volume. Our technique enables simultaneous an optically sectioned image stack with single camera at frame rates hundreds hertz, without need for axial scanning. We demonstrate...
Context.— Histology, the traditional method of examining surgical tissue under a microscope, is time-consuming process involving fixation in formalin, dehydration, embedding paraffin, and cutting into thin sections for hematoxylin-eosin (H&E) staining. Frozen section analysis faster alternative used surgery to quickly evaluate tissue, but it has limitations, such as size specimens that can be analyzed difficulties with fatty bony tissues. Objective.— To rapidly examine nonprocessed...
Multiphoton microscopes are hampered by limited dynamic range, preventing weak sample features from being detected in the presence of strong features, or capture unpredictable bursts strength. We present a digital electronic add-on technique that vastly improves range multiphoton microscope while limiting potential photodamage. The provides real-time negative feedback to regulate laser power delivered sample, and log representation strength accommodate ultrahigh without loss information. No...
The human retina is typically imaged in a reflection geometry, where light delivered through the pupil and images are formed from reflected back retina. In this configuration, artifacts caused by retinal surface reflex often encountered, which complicate quantitative interpretation of images. We present an alternative illumination method, avoids these artifacts. method uses deeply penetrating near-infrared (NIR) transcranially side head, exploits multiple scattering to redirect portion...
Traditional histopathology is a time-intensive and labor-intensive process involving tissue formalin fixation, paraffin embedding, microtoming into thin sections for H&E staining. Frozen section analysis modality used during surgery to quickly evaluate tissue, but it has limitations, such as the size number of specimens that can be analyzed well difficulties with fatty bony tissues. Our objective was investigate performance nonlinear microscopy, fluorescence microscopy technique, rapid...
The fast market growth during the recent years led to huge amounts of production capacities and a large variety module types. increasing need for cost reductions obliges manufacturers maximize efficiencies. Electroluminescence investigation is powerful tool reveal failures thin film photovoltaic modules stage within test sequences. We use it identify after reliability tests included in IEC 61646 or beyond such as bias damp heat assessments. In an earlier stage, manufacturer could sort...
We describe a new technique for non-contact in vivo corneal and lenticular microscopy. It is based on fundus retro-reflection back-illumination of the crystalline lens cornea. To enhance phase-gradient contrast, we apply asymmetric illumination by illuminating one side fundus. The produces micron-scale lateral resolution images across 1 mm diagonal field view central show representative epithelium, subbasal nerve plexus, large stromal nerves, dendritic immune cells, endothelial nuclei,...
Abstract The ability to optically image cellular transmembrane voltage at millisecond-timescale resolution can offer unprecedented insight into the function of living brains in behaving animals. chemigenetic indicator Voltron is bright and photostable, making it a favorable choice for long vivo imaging neuronal populations resolution. Improving sensitivity would allow better detection spiking subthreshold signals. We performed site saturation mutagenesis 40 positions screened increased ΔF/F...
We describe a simple and fast technique to perform ultrasound differential phase contrast (DPC) imaging in arbitrarily thick scattering media. Although configured reflection geometry, DPC is based on transmission direct analog of optical interference contrast. exploits the memory effect works combination with standard pulse-echo imaging, no additional hardware or data requirements, enabling complementary (in transverse direction) without any need for intensive numerical computation....
Thorough examination of renal biopsies may improve understanding disease. Imaging with fluorescence nonlinear microscopy (NLM) and optical clearing enables three-dimensional (3D) visualization pathology without microtome sectioning. Archival paraffin blocks from 12 patients were deparaffinized stained Hoechst Eosin for fluorescent nuclear cytoplasmic/stromal contrast, then optically cleared using benzyl alcohol benzoate (BABB). NLM images entire biopsy fragments (thickness range 88-660 μm)...
ABSTRACT Genetically encoded voltage indicators (GEVIs) hold great promise for monitoring neuronal population activity, but GEVI imaging in dense populations remains difficult due to a lack of contrast and/or speed. To address this challenge, we developed novel confocal microscope that allows simultaneous multiplane with high-contrast at near-kHz rates. This approach enables high signal-to-noise ratio densely labeled and minimizes optical crosstalk during concurrent optogenetic photostimulation.
We present an alternative illumination scheme for retinal imaging. It is based on near-infrared light delivered transcranially at the temple and diffusion towards retina. This unique transmission geometry simplifies absorption measurements enables clear imaging as deep choroid.
Abstract We describe a new technique for non-contact in vivo corneal and lenticular microscopy. It is based on fundus retro-reflection back-illumination of the crystalline lens cornea. To enhance phase-gradient contrast, we apply asymmetric illumination by illuminating one side fundus. The produces micron-scale lateral resolution across 1-mm diagonal field view. show representative images epithelium, subbasal nerve plexus, large stromal nerves, dendritic immune cells, endothelial nuclei,...
We describe a technique to reveal speed-of-sound (SoS) variations within an echogenic sample. The uses the same receive data as standard pulse-echo imaging based on plane-wave compounding, and can be operated in parallel. Point-like scatterers randomly distributed throughout sample serve local probes of downstream transmit-beam phase shifts caused by aberrating structures Phase are monitored differential manner, providing signatures transverse gradients SoS. contrast is augmented method...