A5079872563- Gut microbiota and health
- Escherichia coli research studies
- HIV Research and Treatment
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Infant Nutrition and Health
- Diet and metabolism studies
- Viral gastroenteritis research and epidemiology
- Antibiotic Resistance in Bacteria
- HIV/AIDS oral health manifestations
- Alcoholism and Thiamine Deficiency
- Bacteriophages and microbial interactions
- Biotin and Related Studies
- Probiotics and Fermented Foods
- Folate and B Vitamins Research
- Amino Acid Enzymes and Metabolism
- Microbial infections and disease research
Washington University in St. Louis
2021-2022
North Carolina State University
2012-2019
Universidade Estadual de Londrina
2006
Enterotoxigenic E. coli (ETEC) produce heat-labile (LT) and/or heat-stable (ST) enterotoxins, and commonly cause diarrhea in resource-poor regions. ETEC have been linked repeatedly to sequelae children including enteropathy, malnutrition, growth impairment. Although cellular actions of enterotoxins leading are well-established, their contributions remain unclear. LT increases cAMP activate protein kinase A (PKA) that phosphorylates ion channels driving intestinal export salt water resulting...
Enterotoxigenic Escherichia coli (ETEC) isolates are genetically diverse pathological variants of E. defined by the production heat-labile (LT) and/or heat-stable (ST) toxins. ETEC strains estimated to cause hundreds millions cases diarrheal illness annually.
Hypoxia exerts profound effects on cell physiology, but its effect colonic uptake of the microbiota-generated forms vitamin B1 (i.e., thiamin pyrophosphate [TPP] and free thiamine) has not been described. Here, we used human epithelial NCM460 cells differentiated colonoid monolayers as in vitro ex vivo models, respectively, were subjected to either chamber (1% O2, 5% CO2, 94% N2) or chemically (desferrioxamine; 250 μM)-induced hypoxia followed by determination different...
Using complementary models, including human differentiated enteroid and colonoid monolayers, this study shows the involvement of molecular epigenetic mechanisms in mediating inhibitory effect chronic alcohol exposure on biotin uptake along intestinal tract. The also that metabolites (generated by gut microbiota host tissues) cause inhibition uptake.
Accumulating evidence suggests that natural killer (NK) cells may have an important role in HIV-1 disease pathogenesis; however, vivo studies are lacking. Feline immunodeficiency virus (FIV) infection of cats provides a valuable model to study NK cell function vivo. The immune response against Listeria monocytogenes (Lm) is well characterized, allowing its use as innate probe. We previously shown locally delivered IL-15 can improve Lm clearance FIV-infected animals, and this correlated with...
A hemaglutinina temperatura sensível (Tsh) pertence à família das serino-proteases autotransporte de Enterobacteriacea (SPATE), as quais são capazes clivar diferentes substratos. Nós isolamos e caracterizamos o gene Escherichia coli patogênica aviária (APEC) amostra APEC 13, sorotipo O2:H9,clonado em pET101. região 4.2 kb do DNA clonado codifificou uma proteína aproximadamente 140 kDa (r-Tsh). O plasmídio recombinante pET101-tsh conferiu um fenótipo hemaglutinação positivo para a linhagem...
Regulatory T cells (Treg) are key players in the maintenance of peripheral tolerance, preventing autoimmune diseases and restraining chronic inflammatory diseases. Evidence suggests Treg NK have important roles feline immunodeficiency virus (FIV) pathogenesis; however, vivo studies investigating interplay between these two cell populations lacking. We previously described innate immune defects FIV-infected cats characterized by cytokine deficits impaired natural killer (NK) (NKT) functions....
Abstract Enterotoxigenic Escherichia coli (ETEC) are a genetically diverse pathologic variant of E. defined by the production heat-labile (LT) and/or heat-stable (ST) toxins. ETEC estimated to cause hundreds millions cases diarrheal illness annually. However, it is not clear that all strains equally equipped disease and asymptomatic colonization with common in low-middle income regions lacking basic sanitation clean water where ubiquitous. Recent molecular epidemiology studies have revealed...
abstract Enterotoxigenic E. coli (ETEC), produce heat-labile (LT) and/or heat-stable (ST) enterotoxins, and are a common cause of diarrhea in children resource-poor regions. ETEC have also been linked repeatedly to poorly understood sequelae including enteropathy, malnutrition, growth impairment. While the cellular actions enterotoxins leading well-established, their potential contribution subsequent pathology is unclear. LT stimulates cAMP production activate protein kinase A (PKA) which...