A5040267657- Plant Virus Research Studies
- CRISPR and Genetic Engineering
- Plant tissue culture and regeneration
- Plant Molecular Biology Research
- Plant Reproductive Biology
- RNA Interference and Gene Delivery
- Plant Disease Management Techniques
- Plant Stress Responses and Tolerance
- Plant Water Relations and Carbon Dynamics
- Transgenic Plants and Applications
- Soil Carbon and Nitrogen Dynamics
- Plant Physiology and Cultivation Studies
- Photosynthetic Processes and Mechanisms
- Nematode management and characterization studies
- Growth and nutrition in plants
- Insect Resistance and Genetics
University of Connecticut
2021-2024
The University of Western Australia
2024
Hunan Agricultural University
2021-2023
U.S. National Arboretum
2023
Guangdong Academy of Agricultural Sciences
2023
Abstract Green fluorescent protein (GFP) has been widely used for monitoring gene expression and localization in diverse organisms. However, highly sensitive imaging equipment, like fluorescence microscope, is usually required the visualization of GFP, limitings its application to fixed locations samples. A reporter that can be visualized real-time regardless shape, size location target samples will increase flexibility efficiency research work. Here, we report a GFP-like protein, called...
Abstracts Grafting is a highly useful technique, and its success largely depends on graft union formation. In this study, we found that root-specific expression of the auxin biosynthetic gene iaaM in tobacco, when used as rootstock, resulted more rapid callus formation faster healing. However, overexpression auxin-inactivating iaaL rootstocks delayed We observed increased endogenous levels auxin-responsive DR5::GUS scions WT/ grafts compared with those WT/WT grafts, which suggested...
Abstract Long-distance transport or systemic silencing effects of exogenous biologically active RNA molecules in higher plants have not been reported. Here, we report that cationized bovine serum albumin (cBSA) avidly binds double-stranded beta-glucuronidase (dsGUS RNA) to form nucleic acid–protein nanocomplexes. In our experiments with tobacco and poplar plants, successfully demonstrated gene cBSA/dsGUS nanocomplexes when locally applied the from basal ends leaf petioles shoots. We further...
Genome editing technologies provide a powerful tool for genetic improvement of perennial ryegrass, an important forage and turfgrass species worldwide. The sole publication gene in ryegrass used gene-gun plant transformation dual promoter based CRISPR/Cas9 system editing. However, their efficiency was low (5.9% or only one gene-edited produced). To test the suitability maize Ubiquitin 1 ( ZmUbi1 ) we produced promoter: RUBY transgenic plants. We observed that active callus tissue prior to...
The colorful "delayed leaf greening" is a common but overlooked phenomenon in phosphorus (P)-limited environments habitats tropical, subtropical and temperate forests, the physiological mechanism underpinning it remains unclear. It important to understand how allocation of major P fractions shifts during development, as strategy for utilizing efficiently. We measured concentrations nitrogen five chemical fractions, metal element (K, Ca, Mg, Mn, Fe, Cu, Zn Se) young mature leaves six woody...
Abstract Deciduous woody plants like poplar (Populus spp.) have seasonal bud dormancy. It has been challenging to simultaneously delay the onset of dormancy in fall and advance break spring, as dormancy, were thought be controlled by different genetic factors. Here, we demonstrate that heterologous expression REVEILLE1 gene (named AaRVE1) from Agave (Agave americana) not only delays but also accelerates field trials. AaRVE1 increases biomass yield 166% greenhouse. Furthermore, reveal...
Abstract Promoting initiation and biomass production of roots is significant for plant-based industries including using as bioreactors. Two citrus genotypes, Carrizo US-897, were used model plants to test the effects root-predominantly overexpressed iaaM (indoleacetic acid-tryptophan monooxygenase) gene a CKX (a cytokinin oxidase/dehydrogenase) gene. The transgenic lines exhibited markedly faster root initiation, more numbers, higher compared their wild-type counterparts. + also similar...
Abstract Promoting initiation and biomass production of roots is significant for plant-based industries including using as bioreactors. We used two citrus genotypes, Carrizo US-897, model plants to test the effects root-predominantly overexpressed iaaM (indoleacetic acid-tryptophan monooxygenase) gene a CKX (a cytokinin oxidase/dehydrogenase) gene. The transgenic lines exhibited markedly faster root initiation, more numbers, higher compared their wild-type counterparts. + also similar...
Abstract Long-distance transport of exogenous biologically active RNA molecules in higher plants has not been reported. Here, we report that cationized bovine serum albumin (cBSA) avidly binds double-stranded beta-glucuronidase (dsGUS RNA) to form nucleic acid-protein nanocomplexes. Using tobacco and poplar plants, have shown effective uptake long-distance cBSA/dsGUS nanocomplexes from basal ends leaf petioles blades as well shoots their apexes apical leaves. We further demonstrated the are...