A5002327189- Plant Stress Responses and Tolerance
- Plant Micronutrient Interactions and Effects
- Plant nutrient uptake and metabolism
- Plant Molecular Biology Research
- Plant Virus Research Studies
- Plant Reproductive Biology
- Plant responses to water stress
- Plant tissue culture and regeneration
- Plant Physiology and Cultivation Studies
- RNA Interference and Gene Delivery
- Legume Nitrogen Fixing Symbiosis
- CRISPR and Genetic Engineering
- Nematode management and characterization studies
- Photosynthetic Processes and Mechanisms
- Transgenic Plants and Applications
- Plant-Microbe Interactions and Immunity
- Plant Disease Management Techniques
- 14-3-3 protein interactions
- Plant Gene Expression Analysis
- Mycorrhizal Fungi and Plant Interactions
China Agricultural University
2016-2025
Ministry of Agriculture and Rural Affairs
2022-2025
University of Connecticut
2019-2024
Hunan Agricultural University
2023
U.S. National Arboretum
2023
Guangdong Academy of Agricultural Sciences
2023
Due to the chelation of phosphorus in soil, it becomes unavailable for plant growth and development. The mechanisms by which phosphorus-solubilizing bacteria activate immobilized promote development woody plants, as well intrinsic molecular mechanisms, are not clear. Through analysis microbial communities rhizosphere 16S V3-V4 a homologous gene encoding alkaline phosphomonoesterase (phoD) phosphate-efficient (PE) phosphate-inefficient apple rootstocks, was found that PE significantly...
Abstract Plants experiencing salt‐induced stress often reduce cytokinin levels during the early phases of stress‐response. Interestingly, we found that content in apple rootstock “robusta” was maintained at a high level under salt stress. Through screening genes involved biosynthesis and catabolism, expression IPT5b robusta roots were maintaining content. We identified 42 bp deletion promoter region , which elevated levels, this linked to tolerance robusta×M.9 segregating population. The...
Abstract Iron (Fe) is a trace element necessary for plant growth. Many land plants have evolved set of mechanisms associated with the Fe absorption process to deal problem insufficient supply in soil. During absorption, reactive oxygen species (ROS) can be used as signal initiate response stress caused by deficiency. However, molecular underlying involvement ROS deficiency remains unclear. In this study, we identified kinase, MxMPK6-2, from Malus xiaojinensis, an apple rootstock that highly...
Abstracts Grafting is a highly useful technique, and its success largely depends on graft union formation. In this study, we found that root-specific expression of the auxin biosynthetic gene iaaM in tobacco, when used as rootstock, resulted more rapid callus formation faster healing. However, overexpression auxin-inactivating iaaL rootstocks delayed We observed increased endogenous levels auxin-responsive DR5::GUS scions WT/ grafts compared with those WT/WT grafts, which suggested...
Phosphorus in the soil is easily chelated into forms that are unavailable to plants, leading phosphorus deficiency, which severely affects growth, development, and fruit quality of apple trees. To address we used four different arbuscular mycorrhizal fungi (AMF) investigate their effects on growth development rootstocks uptake soil. We identified Glomus mosseae (Gm) as most effective AMF for promoting found under phosphorus-deficient conditions, inoculating with Gm promoted above-ground...
Reactive oxygen species (ROS) are important signaling molecules in plants that contribute to stress adaption. This study demonstrated ROS play a critical role Fe deficiency-induced at an early stage Malus xiaojinensis. Once production has been initiated, prolonged starvation leads activation of scavenging mechanisms. Further, we scavengers involved maintaining the cellular redox homeostasis during deficiency treatment. Taken together, our results describe feedback repression loop for...
Understanding the mechanism of iron (Fe)-deficiency responses is crucial for improving plant Fe bioavailability. Here, we found that Arabidopsis Rho-like GTPase 6 mutant (rop6) less sensitive to Fe-deficiency and has reduced levels reactive oxygen species (ROS) compared wild-type (WT), while AtROP6-overexpressing seedlings exhibit more sensitivity higher ROS WT. Moreover, treatment with H2 O2 improves in rop6 mutants. By using yeast two-hybrid system, further demonstrate direct interaction...
Abstract Iron (Fe) deficiency limits the yield of fruit trees. When subjected to Fe deficiency, H+ secretion increases in rhizosphere dicotyledonous plants and pH decreases. This leads acidification soil promotes Fe3+ Fe2+ conversion, which can better uptake. study investigated relationship between two inhibitory transcription factors (ethylene response MbERF4 MbERF72) H+-ATPase gene MbHA2. Two species apple woody were studied: Fe-inefficient Malus baccata Fe-efficient xiaojinensis. Yeast...
Abstract Iron (Fe) deficiency is a nutritional stress in plants that commonly occurs alkaline and calcareous soils. Mitogen‐activated protein kinases (MPKs), the terminal player of MAPK cascade, are involved distinct physiological processes. Once suffer from Fe stress, mechanism MPK function remains unclear owing to limited study on networks including substrate proteins downstream pathways. Here, MAP kinase MPK4‐1 was induced roots efficient apple rootstock Malus xiaojinensis but not...
Abstract Long-distance transport or systemic silencing effects of exogenous biologically active RNA molecules in higher plants have not been reported. Here, we report that cationized bovine serum albumin (cBSA) avidly binds double-stranded beta-glucuronidase (dsGUS RNA) to form nucleic acid–protein nanocomplexes. In our experiments with tobacco and poplar plants, successfully demonstrated gene cBSA/dsGUS nanocomplexes when locally applied the from basal ends leaf petioles shoots. We further...
SUMMARY Iron (Fe) deficiency significantly affects the growth and development, fruit yield quality of apples. Apple roots respond to Fe stress by promoting H + secretion, which acidifies soil. In this study, plasma membrane (PM) ‐ATPase MxHA2 promoted secretion root acidification apple rootstocks under stress. is upregulated in Fe‐efficient rootstock Malus xiaojinensis at transcription level . also induced kinase MxMPK6‐2, a positive regulator absorption that can interact with MxHA2....
Abstract Iron (Fe) deficiency is a long-standing issue in plant mineral nutrition. Ca2+ signals and the mitogen-activated protein kinase (MAPK) cascade are frequently activated parallel to perceive external cues, but their interplay under Fe stress remains largely unclear. Here, MxMPK4-1, which induced during response apple rootstock Malus xiaojinensis, cooperates with IQ-motif containing protein3 (MxIQM3). MxIQM3 gene expression, abundance, phosphorylation level increased stress. The...
Abstract Apple rootstock dwarfing and dense planting are common practices in apple farming. However, the mechanisms not understood. In our study, expression of MdARF3 root system ‘M9’ was lower than vigorous from Malus micromalus due to deletion WUSATAg element promoter genotype. Notably, this variation significantly associated with rootstocks. Subsequently, transgenic tobacco (Nicotiana tabacum) cv. Xanthi generated ARF3 M. genotypes. The 35S::MdARF3 also obtained. highly expressed had a...
The micronutrient iron plays a crucial role in the growth and development of plants, necessitating meticulous regulation for its absorption by plants. Prior research has demonstrated that transcription factor MxZR3.1 restricts apple rootstocks; however, precise mechanism which contributes to homoeostasis rootstocks remains unexplored. Here, MxMPK3-2, protein kinase, was discovered interact with MxZR3.1. Y2H, bimolecular fluorescence complementation pull down experiments were used confirm...
Abstract Promoting initiation and biomass production of roots is significant for plant-based industries including using as bioreactors. Two citrus genotypes, Carrizo US-897, were used model plants to test the effects root-predominantly overexpressed iaaM (indoleacetic acid-tryptophan monooxygenase) gene a CKX (a cytokinin oxidase/dehydrogenase) gene. The transgenic lines exhibited markedly faster root initiation, more numbers, higher compared their wild-type counterparts. + also similar...
Abstract Promoting initiation and biomass production of roots is significant for plant-based industries including using as bioreactors. We used two citrus genotypes, Carrizo US-897, model plants to test the effects root-predominantly overexpressed iaaM (indoleacetic acid-tryptophan monooxygenase) gene a CKX (a cytokinin oxidase/dehydrogenase) gene. The transgenic lines exhibited markedly faster root initiation, more numbers, higher compared their wild-type counterparts. + also similar...
Abstract Long-distance transport of exogenous biologically active RNA molecules in higher plants has not been reported. Here, we report that cationized bovine serum albumin (cBSA) avidly binds double-stranded beta-glucuronidase (dsGUS RNA) to form nucleic acid-protein nanocomplexes. Using tobacco and poplar plants, have shown effective uptake long-distance cBSA/dsGUS nanocomplexes from basal ends leaf petioles blades as well shoots their apexes apical leaves. We further demonstrated the are...
ABSTRACT Small G protein is a multifunctional molecular switch that can regulate plant growth, development and responses to the environment. However, how Rho-related GTPase of plants (ROPs) regulates response Fe deficiency has not been well clarified. Here, we found induced MxROP1 in Malus xiaojinensis at both transcriptional translational levels. The overexpression MxROP1, DN (inactive form) CA (active apple roots increased activity ferric chelate reductase ability acidify rhizosphere,...