A5075607809- Liver physiology and pathology
- Plant Physiology and Cultivation Studies
- Liver Disease Diagnosis and Treatment
- Pancreatic function and diabetes
- Liver Disease and Transplantation
- Plant Reproductive Biology
- Organ Transplantation Techniques and Outcomes
- Postharvest Quality and Shelf Life Management
- Endoplasmic Reticulum Stress and Disease
- Adenosine and Purinergic Signaling
- Horticultural and Viticultural Research
- Diet, Metabolism, and Disease
- Rice Cultivation and Yield Improvement
- Phytase and its Applications
- Pancreatitis Pathology and Treatment
- Plant responses to water stress
- Wnt/β-catenin signaling in development and cancer
- Pain Mechanisms and Treatments
- Ion channel regulation and function
- Enzyme Production and Characterization
- Agriculture and Biological Studies
- Potato Plant Research
- Flowering Plant Growth and Cultivation
- Agricultural Systems and Practices
- Seed and Plant Biochemistry
University of Shizuoka
1988-2025
Keio University
2024
Pharmac
2019-2023
Graco (United States)
2023
Institute of Fruit Tree and Tea Science
2001-2007
Tokyo University of Agriculture
1999
Central Research Institute
1996
Fuji Oil (Japan)
1996
Musashigaoka College
1988
The improvement of type 2 diabetes mellitus induced by naturally occurring polyphenols, known as flavonoids, has received considerable attention. However, there is a dearth information regarding the effect trihydroxyflavone apigenin on pancreatic β-cell function. In present study, anti-diabetic insulin secretion, apoptosis, and mechanism underlying its effects, were investigated in INS-ID line. results showed that concentration-dependently facilitated 11.1-mM glucose-induced which peaked at...
Introduction Nobiletin is a polymethoxyflavonoid abundant in citrus peels and has been reported to have various bioactive effects. We previously that nobiletin inhibits endoplasmic reticulum stress-induced apoptosis the pancreatic β-cell line INS-1 continuous subcutaneous administration of suppresses progression diabetes by protecting β-cells type 2 diabetic db/db mice. In present study, we investigated effects oral ingestion Shiikuwasha extract rich on pathogenesis Materials methods A was...
During liver injury, hepatic stellate cells (HSCs) are activated by various cytokines and transdifferentiated into myofibroblast-like HSCs, which produce collagen, a major source of fibrosis. Therefore, the suppression HSC activation is regarded as therapeutic target for Several epidemiological reports have revealed that caffeine intake decreases risk disease. In this study, therefore, we investigated effect on primary HSCs isolated from mice. Caffeine suppressed in concentration-dependent...
Abstract The increased incidence of obesity in the global population has risk several chronic inflammation-related diseases, including non-alcoholic steatohepatitis (NASH)-hepatocellular carcinoma (HCC). progression from NASH to HCC involves a virus-independent liver carcinogenic mechanism; however, we currently lack effective treatment and prevention strategies. Several reports have suggested that fecal volatile organic compounds (VOCs) are strongly associated with NASH-HCC; therefore,...
Liver inflammation leads to the activation of hepatic stellate cells (HSCs), resulting in development liver fibrosis. The present study aimed investigate effects prostaglandin E2 (PGE2), which is biosynthesized by Kupffer cells, hepatocytes, and HSCs during inflammation, on HSC activation, including its combinatory effect with caffeine.HSCs isolated from mice were activated culturing a medium supplemented 10% fetal bovine serum for 7 days plastic plates. was evaluated immunofluorescence...
Myofibroblast-like activated hepatic stellate cells (aHSCs), which produce collagen, a major cause of liver fibrosis, are specific target for antifibrotic treatment. Recently, several reports have indicated that extracellular vesicles (EVs) play important roles in cell-to-cell communication through their tropism or organs. Therefore, the present study aimed to identify aHSC-directed EVs by focusing on interactions under pathological conditions. were derived from hepatocyte cell line AML12...
The preventive effect of beta-alanyl-L-histidinato zinc (AHZ) on the deterioration bone metabolism was investigated in femoral diaphysis ovariectomized rats. AHZ (10, 30 and 100 mg/kg body weight/d) orally administered to rats for 6 weeks. Ovariectomy produced a significant decrease estradiol, calcitonin, calcium inorganic phosphorus concentrations serum as compared with those from sham-operated dose mg AHZ/kg prevented any concentration caused by ovariectomy. Alkaline phosphatase activity,...
The biological effect of calcitonin (CT) was investigated in isolated rat hepatocytes. Addition synthetic [Asu1,7] eel CT (20 and 40 ng/ml medium with 2.5 mg dry weight cells) into the containing calcium ion produced a marked elevation uptake hepatocytes, when concentrations were monitored spectrophotometrically using arsenazo III. Intracellular contents mitochondria microsomes significantly increased by addition. Meanwhile, glycogenolysis gluconeogenesis hepatocytes stimulated addition (10,...
Although quiescent hepatic stellate cells (HSCs) have been suggested to regulate blood flow, there is no direct evidence that HSCs display contractile abilities. Here, we developed a new method quantitatively measure the contraction of single isolated and evaluated whether endothelin-1 (ET-1) induced in non-activated state. from mice were seeded on collagen gel containing fluorescent beads. The beads around HSC observed gravitating toward cell upon contraction. By recording movement each...
The substrate specificity of a novel serine protease isolated from soybean seeds, cultivar Keburi, was investigated using various peptide-MCAs and several neuropeptides involving single paired basic ami no acid sequences. quite specific for arginine residue at the PI site active center, it recognized Arg-Arg cleaved linkage between or after in peptide protein molecules. This is first plant tissues which resembles arginine-specific proteinases porcine gastric intestinal mucosa, recognizing amino
Abstract Unique architectures of microbial skeletons are viewed as a model for the architectural design artificial structural materials. In particular, specific geometric arrangement spherical skeleton 0.5–1.5 mm in diameter shell-bearing protists, Phaeodaria ( Aulosphaera sp.), is remarkably interesting because its similarity to geodesic polyhedron, which hollow framework with 6-branched nodes that requires minimal building material maximal strength. A phaeodarian composed silica rods 5–10...