A5064741037- Mitochondrial Function and Pathology
- Cancer, Hypoxia, and Metabolism
- Adipose Tissue and Metabolism
- ATP Synthase and ATPases Research
- Metabolism and Genetic Disorders
- Diet and metabolism studies
- Metabolomics and Mass Spectrometry Studies
- Luminescence Properties of Advanced Materials
- Fatty Acid Research and Health
- RNA modifications and cancer
- Inorganic Fluorides and Related Compounds
- Amino Acid Enzymes and Metabolism
- Epigenetics and DNA Methylation
- Pancreatic function and diabetes
- Free Radicals and Antioxidants
- Sirtuins and Resveratrol in Medicine
- Advanced Fluorescence Microscopy Techniques
- Lipid metabolism and biosynthesis
- Cell Image Analysis Techniques
- Enzyme Structure and Function
- Traditional Chinese Medicine Analysis
- Pain Management and Opioid Use
- MicroRNA in disease regulation
- Neurological diseases and metabolism
- Advanced Electron Microscopy Techniques and Applications
Czech Academy of Sciences, Institute of Physiology
2015-2025
Czech Academy of Sciences
2015-2025
St. Anne's University Hospital Brno
2024
University Hospital Brno
2024
Masaryk University
2024
University of Guelph
2017
Bordeaux Population Health
2008-2010
Inserm
2008-2010
Université de Bordeaux
2008
The recent development of diffraction-unlimited far-field fluorescence microscopy has overcome the classical resolution limit ~250 nm conventional light by about a factor ten. improved resolution, however, reveals not only biological structures at an unprecedented but is also susceptible to sample drift on much finer scale than previously relevant. Without correction, leads smeared images with decreased and in worst case misinterpretation imaged structures. This poses problem especially for...
Abstract Background Pancreatic ductal adenocarcinoma (PDAC) has been associated with the host dysmetabolism of branched-chain amino acids (BCAAs), however, implications for role BCAA metabolism in PDAC development or progression are not clear. The mitochondrial catabolism valine, leucine, and isoleucine is a multistep process leading to production short-chain R-CoA species. They can be subsequently exported from mitochondria as carnitines (SC-CARs), utilized anabolic pathways, released...
Isocitrate dehydrogenase 2 (IDH2) is located in the mitochondrial matrix. IDH2 acts forward Krebs cycle as an NADP(+)-consuming enzyme, providing NADPH for maintenance of reduced glutathione and peroxiredoxin systems self-maintenance by reactivation cystine-inactivated glutaredoxin 2. In highly respiring cells, resulting NAD(+) accumulation then induces sirtuin-3-mediated activating deacetylation, thus increasing its protective function. Reductive carboxylation 2-oxoglutarate (in reverse...
The relationship of the inner mitochondrial membrane (IMM) cristae structure and intracristal space (ICS) to oxidative phosphorylation (oxphos) is not well understood. Mitofilin (subunit Mic60) contact site organizing system (MICOS) IMM complex attached outer (OMM) via sorting assembly machinery/topogenesis β-barrel proteins (SAM/TOB) controls shape cristae. ATP synthase dimers determine sharp edges, whereas trimeric OPA1 tightens ICS outlets. Metabolism altered during hypoxia, we therefore...
Aims: Glucose-stimulated insulin secretion (GSIS) in pancreatic β cells was expected to enhance mitochondrial superoxide formation. Hence, we elucidated relevant redox equilibria. Results: Unexpectedly, INS-1E at transitions from 3 (11 mM; islets 5 mM) 25 mM glucose decreased matrix release rates (MitoSOX Red monitoring validated by MitoB) and H2O2 (mitoHyPer, subtracting mitoSypHer emission). Novel double-channel fluorescence lifetime imaging, approximating free NADHF, indicated its ∼20%...
Oleic acid-stabilized hexagonal NaYF4:Yb3+/Er3+ nanocrystals, emitting green and red luminescence, were prepared by the high-temperature co-precipitation of lanthanide chlorides. By varying reaction time Ln3+/Na+ ratio, nanocrystal size can be controlled within range 16–270 nm. The maximum upconversion quantum yield is achieved under 970 nm excitation. reverse microemulsion technique using hydrolysis condensation tetraethoxysilane a suitable method to coat surface with silica shell make...
Background Local anesthetics offer the benefits of extended analgesia with greater patient satisfaction and faster rehabilitation compared intravenous morphine. These benefits, however, can be offset by adverse iatrogenic muscle pain caused bupivacaine. Here, authors describe mechanisms local anesthetic-induced myotoxicity a partial protective effect recombinant human erythropoietin (rhEPO). Methods The developed rat model femoral nerve catheter cell culture skeletal myoblasts to study...
The glycaemic response of millet foods and the effect processing are not known. Therefore, decorticated proso was used to produce four types common food products (biscuits, couscous, porridge an extruded snack). Postprandial blood glucose these (all containing 50 g total starch) compared same produced with refined corn, in a crossover human study 12 healthy male participants (age 26.3 ± 3.8 yr; BMI 23.3 2.8 kg/m2). Capillary samples were collected determined; differences assessed using...
Mitochondrial Ca2+-independent phospholipase A2γ (iPLA2γ/PNPLA8) was previously shown to be directly activated by H2O2 and release free fatty acids (FAs) for FA-dependent H+ transport mediated the adenine nucleotide translocase (ANT) or uncoupling protein 2 (UCP2). The resulting mild mitochondrial consequent partial attenuation of superoxide production lead an antioxidant effect. However, role iPLA2γ in brain is not completely understood. Here, using wild-type iPLA2γ-KO mice, we demonstrate...
Each cell types or tissues contain certain “physiological” levels of R-2-hydroxyglutarate (2HG), as well enzymes for its synthesis and degradation. 2HG accumulates in tumors, possessing heterozygous point mutations isocitrate dehydrogenases IDH1 (cytosolic) IDH2 (mitochondrial) contributes to strengthening their malignancy by inhibiting 2-oxoglutarate-dependent dioxygenases. By blocking histone de-methylation 5-methyl-cytosine hydroxylation, maintains cancer cells de-differentiated promotes...
Abstract Wild type mitochondrial isocitrate dehydrogenase (IDH2) was previously reported to produce oncometabolite 2- hydroxyglutarate (2HG). Besides, deacetylase SIRT3 has been shown regulate the oxidative function of IDH2. However, regulation 2HG formation by SIRT3-mediated deacetylation not investigated yet. We aimed study IDH2 in response acetylation and deacetylation, focus specifically on production used surrogate mutant K413Q assayed enzyme kinetics decarboxylation isocitrate, enzyme,...