A5058736373- Effects of Radiation Exposure
- Advanced Radiotherapy Techniques
- Advances in Oncology and Radiotherapy
- Ultrasound and Hyperthermia Applications
- Radiation Therapy and Dosimetry
- Bladder and Urothelial Cancer Treatments
- Angiogenesis and VEGF in Cancer
- Urological Disorders and Treatments
- Metabolomics and Mass Spectrometry Studies
- Lung Cancer Diagnosis and Treatment
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Bioinformatics and Genomic Networks
- Cancer, Hypoxia, and Metabolism
- TGF-β signaling in diseases
- Cell Image Analysis Techniques
- PARP inhibition in cancer therapy
- Nanoplatforms for cancer theranostics
- Radioactivity and Radon Measurements
- Pelvic floor disorders treatments
- Cancer Immunotherapy and Biomarkers
- Circadian rhythm and melatonin
- Urinary and Genital Oncology Studies
- Glutathione Transferases and Polymorphisms
- Heat shock proteins research
- Biomarkers in Disease Mechanisms
Institut de Radioprotection et de Sûreté Nucléaire
2008-2024
Greater Poland Cancer Center
2019
CEA Paris-Saclay - Etablissement de Fontenay-aux-roses
2018
Université d'Évry Val-d'Essonne
2014
Laboratoire de Recherche en Informatique
2014
Centre National de la Recherche Scientifique
2014
Institut national de recherche en informatique et en automatique
2014
Informatique, BioInformatique, Systèmes Complexes
2014
Université Paris-Sud
2014
Hôpital Necker-Enfants Malades
1999
To investigate the nature of somatic von Hippel-Lindau (VHL) mutations, we analyzed 173 primary sporadic human renal cell carcinomas for mutations VHL tumor suppressor gene, using polymerase chain reaction (PCR) and single-strand conformational polymorphism analysis (SSCP) DNA. We detected abnormal SSCP pattern in 73 samples. After sequencing, identified microdeletions 58% cases, microinsertions 17%, nonsense 8%, missense 17%. Among these 50% correspond to new mutations. were found only...
Abstract The pathophysiological mechanism involved in side effects of radiation therapy and especially the role endothelium remains unclear. Previous results showed that plasminogen activator inhibitor-type 1 (PAI-1) contributes to radiation-induced intestinal injury suggested this could be driven by an endothelium-dependent mechanism. We investigated whether endothelial-specific PAI-1 deletion affect injury. created a mouse model with specific (PAI-1KO endo ) Cre-LoxP system. In...
Normal tissue toxicity still remains a dose-limiting factor in clinical radiation therapy. Recently, plasminogen activator inhibitor type 1 (SERPINE1/PAI-1) was reported as an essential mediator of late radiation-induced intestinal injury. However, it is not clear whether PAI-1 plays role acute damage and we hypothesized that may play the endothelium radiosensitivity. In vivo, model enteropathy −/− mice, apoptosis radiosensitive compartments, epithelial microvascular quantified. vitro,...
Abstract The efficacy and side effects of radiotherapy (RT) depend on parameters like dose the volume irradiated tissue. RT induces modulations tumor immune microenvironment (TIME) that are dependent dose. Low (LDRT, i.e., single doses 0.5–2 Gy) has been shown to promote infiltration into tumor. Here we hypothesize partial irradiation combining immunostimulatory/non-lethal properties LDRT with cell killing/shrinkage high (HDRT) within same mass could enhance anti-tumor responses when...
Radiation-induced lesion outcomes of normal tissues are difficult to predict. In particular, radiotherapy or local exposure a radioactive source by accident can trigger strong injury the skin. The finding biomarkers is fundamental relevance for prediction apparition and its evolution, settlement therapeutic strategies. order study radiation-induced cutaneous lesions, we developed mouse model in which dorsal skin was selectively exposed ionizing radiation (IR). 2-D difference gel...
Abstract Motivation: Identifying the set of genes differentially expressed along time is an important task in two-sample course experiments. Furthermore, estimating at which periods differential expression present can provide additional insight into temporal gene functions. The current detection methods are designed to detect difference observation intervals or on single measurement points, warranting dense measurements characterize full patterns. Results: We propose a novel Bayesian...
As it is altered by ionizing radiation, the vascular network considered as a prime target in limiting normal tissue damage and improving tumor control radiation therapy. Irradiation activates endothelial cells which then participate recruitment of circulating cells, especially overexpressing cell adhesion molecules, but also other yet unknown mechanisms. Since protein glycosylation an important determinant adhesion, we hypothesized that could alter pattern thereby impact cells. Herein, show...
Background & AimsRadiation therapy in the pelvic area is associated with side effects that impact quality of life cancer survivors. Interestingly, gastrointestinal tract able to adapt significant changes oxygen availability, suggesting mechanisms related hypoxia sensing help preserve tissue integrity this organ. However, hypoxia-inducible factor (HIF)-dependent responses radiation-induced gut toxicity are unknown. Radiation-induced intestinal a complex process involving multiple cellular...
The current study evaluated the role of Hey2 transcription factor in radiation-induced endothelial-to-mesenchymal transition (EndoMT) and its impact on tissue damage mice. Phenotypic modifications irradiated, siRNA- vector plasmid-transfected human umbilical vein endothelial cells (HUVECs) resembling EndoMT were monitored by qPCR, immunocytochemistry western blots. Subsequently, mice, a Cre-LoxP strategy for inactivation specifically endothelium was used to biological consequences. Total...
Exposure of the skin to ionizing radiation leads characteristic reactions that will often turn into a pathophysiological process called cutaneous syndrome. The study this disorder is crucial finding diagnostic and prognostic bioindicators local exposure or effects. It known irradiation alters serum proteome content potentially post-translationally modifies proteins. In study, we investigated whether localized glycome. Two-dimensional differential in-gel electrophoresis proteins from man mice...
Despite advances in radiation delivery protocols, exposure of normal tissues during the course therapy remains a limiting factor cancer treatment. If canonical TGF-β/Smad pathway has been extensively studied and implicated development damage various organs, precise modalities its activation following remain elusive. In present study, we hypothesized that TGF-β1 signaling target genes expression may depend on radiation-induced modifications Smad transcriptional co-repressors/inhibitors...
Whereas an RBE > 1 is described for very low-energy X-ray beams (in the range of 25-50 kV), there a consensus that X-rays (from 0.1 to 3 MeV) equal 1, whatever energy or dose rate beam. Comparisons beam rates are scarce even though these widely used in medical diagnosis radiotherapy. By using two (0.63 and 2.5 Gy.min-1) high-energy on normal endothelial cells (HUVECs), we have studied clonogenic assay, but also viability/mortality, cell cycle analysis measured cellular senescence by flow...
Purpose: To examine the effects of low-dose exposure to uranium with a systems biology approach, multiscale high-throughput multi-omics analysis was applied protocol for chronic rat kidney. Methods: Male and female rats were contaminated nine months through their drinking water nontoxic solution uranyl nitrate. A approach enabled clinical monitoring associated metabolomic transcriptomic (mRNA microRNA) analyses. Results: sex-interaction effect observed in kidney, urine, plasma metabolomes...
The vascular endothelium is a hot spot in the response to radiation therapy for both tumors and normal tissues. To improve patient outcomes, interpretable systemic hypotheses are needed help radiobiologists oncologists propose endothelial targets that could protect tissues from adverse effects of and/or enhance its antitumor potential. this end, we captured kinetics multi-omics layers-i.e. miRNome, targeted transcriptome, proteome, metabolome-in irradiated primary human cells cultured vitro....
PurposeStereotactic body radiation therapy is a therapeutic option offered to high surgical risk patients with lung cancer. Focal irradiation in mice new preclinical model help understand the development of damage this context. Here we developed mouse stereotactic using arc delivery and monitored while varying beam size dose delivered.Methods MaterialsC57BL/6JRj were exposed 90 Gy focal on left 1-mm diameter, 3 × mm2, 7 or 10 mm2 collimation for effect delivering 20 120 effect. Long-term was...
The vascular endothelium is considered as a key cell compartment for the response to ionizing radiation of normal tissues and tumors, promising target improve differential effect radiotherapy in future. Following exposure, global endothelial covers wide range gene, miRNA, protein metabolite expression modifications. Changes occur at transcriptional, translational post-translational levels impact phenotype well microenvironment by production secretion soluble factors such reactive oxygen...