A5062608849- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- DNA Repair Mechanisms
- HIV Research and Treatment
- Glycosylation and Glycoproteins Research
- Ubiquitin and proteasome pathways
- Cancer Genomics and Diagnostics
- Genetic factors in colorectal cancer
- DNA and Nucleic Acid Chemistry
- Immunodeficiency and Autoimmune Disorders
- Advanced NMR Techniques and Applications
- CRISPR and Genetic Engineering
- Enzyme Structure and Function
- Glioma Diagnosis and Treatment
- Solid-state spectroscopy and crystallography
- Cancer-related gene regulation
- Amino Acid Enzymes and Metabolism
- Epigenetics and DNA Methylation
- interferon and immune responses
- RNA modifications and cancer
- RNA regulation and disease
- Electron Spin Resonance Studies
- Bacteriophages and microbial interactions
- Nuclear Structure and Function
- Genetics, Aging, and Longevity in Model Organisms
Institute for Basic Science
2023-2024
Ulsan National Institute of Science and Technology
2024
Max Planck Society
2012-2017
National University of Singapore
2016-2017
Agency for Science, Technology and Research
2016-2017
Institute of Molecular and Cell Biology
2016-2017
Bioinformatics Institute
2016-2017
The University of Texas Health Science Center at San Antonio
2017
Audie L. Murphy Memorial VA Hospital
2017
Friedrich Miescher Laboratory
2016-2017
SPT5 and its binding partner SPT4 regulate transcriptional elongation by RNA polymerase II. are involved in both 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB)-mediated inhibition the activation of human immunodeficiency virus type 1 (HIV-1) Tat protein. Recent data suggest that P-TEFb, which is composed CDK9 cyclin T1, also critical regulating SPT5. In this study, we analyze domains presence either DRB or HIV-1 We demonstrate bind II, addition to a region C terminus contains...
Abstract Most genotoxic anticancer agents fail in tumors with intact DNA repair. Therefore, trabectedin, anagent more toxic to cells active repair, specifically transcription-coupled nucleotide excision repair (TC-NER), provides therapeutic opportunities. To unlock the potential of trabectedin and inform its application precision oncology, an understanding mechanism drug’s TC-NER-dependent toxicity is needed. Here, we determine that abortive TC-NER trabectedin-DNA adducts forms persistent...
Assembly of the HIV and other retroviruses is primarily driven by oligomerization Gag polyprotein, major viral structural protein capable forming virus-like particles even in absence all virally encoded components. Several critical determinants are located C-terminal domain capsid (CA-CTD), which encompasses most conserved segment highly variable called homology region (MHR). The CA-CTD thought to function as a dimerization module, although existing model does not readily explain why...
Cohesin is a protein complex that forms ring around sister chromatids thus holding them together. The composed of three proteins: Smc1, Smc3 and Scc1. roles additional proteins associate with the ring, Scc3, Pds5 Wpl1, are not well understood. It has been proposed these factors form stabilizes prevents it from opening. This activity promotes chromatid cohesion but at same time poses an obstacle for initial entrapment DNAs. hindrance to establishment overcome during DNA replication via...
The RAG1 and RAG2 proteins catalyze V(D)J recombination are essential for generation of the diverse repertoire antigen receptor genes effective immune responses. is composed a "core" domain that required reaction C-terminal nonessential or "non-core" region. Recent evidence has emerged arguing non-core region plays critical regulatory role in reaction, mutations this have been identified patients with immunodeficiencies. Here we present first structural data protein, using NMR spectroscopy...
Cells constantly accumulate mutations, which are caused by replication errors, as well through the action of endogenous and exogenous DNA-damaging agents. Mutational patterns reflect status DNA repair machinery history genotoxin exposure a given cellular clone. Computationally derived mutational signatures can shed light on origins cancer. However, to understand etiology cancer signatures, they need be compared with experimental obtained from isogenic cell lines or organisms under controlled...
The HIV-1 (human immunodeficiency virus type 1) and HIV-2 Tat proteins increase the level of transcription from their corresponding long terminal repeats. activates likely by interaction with components transcriptional initiation elongation complexes during different stages reaction. In current study, two approaches were used to address sites at which becomes stably associated HIV complex. First, we isolated column purified that competent for in vitro found wild-type but not mutant protein...
During cell division, segregation of sister chromatids to daughter cells is achieved by the poleward pulling force microtubules, which attach means a multiprotein complex, kinetochore. Kinetochores assemble at centromeric DNA organized specialized nucleosomes. In contrast other eukaryotes, typically have large repetitive regions, budding yeast CEN defined 125 bp sequence and assembles single nucleosome. yeast, as well in Cse4 histone variant (known vertebrates CENP-A) believed substitute for...
Pure quadrupole resonance is a potentially useful spectroscopic approach to study the coordination of quadrupolar nuclei in biological systems. We used field-cycling NMR method observe boron pure two peptide boronic acid inhibitors bound α-lytic protease. The similar our earlier experiment [Ivanov, D., and Redfield, A. R. (1998) Z. Naturforsch. A 53, 269−272] but uses simple Hartmann−Hahn transfer from proton 11B before field cycle direct after it. sensitive geometry. For example, trigonal...