A5051181238- Proteins in Food Systems
- Enzyme Catalysis and Immobilization
- Biofuel production and bioconversion
- Chemical and Physical Properties in Aqueous Solutions
- Protein purification and stability
- Crystallization and Solubility Studies
- Glycosylation and Glycoproteins Research
- Agricultural and Food Sciences
- Microbial Metabolites in Food Biotechnology
- Polysaccharides Composition and Applications
- Thermodynamic properties of mixtures
- Meat and Animal Product Quality
- Enzyme Production and Characterization
- Enzyme Structure and Function
- Electrospun Nanofibers in Biomedical Applications
- Protein Hydrolysis and Bioactive Peptides
- Microbial Metabolic Engineering and Bioproduction
- Microencapsulation and Drying Processes
- Botanical Research and Applications
- Pineapple and bromelain studies
- Nutritional Studies and Diet
- Food Drying and Modeling
- Drug Solubulity and Delivery Systems
- Agricultural Engineering and Mechanization
- Geography and Environmental Studies
Universidade Federal de Lavras
2017-2025
Universidade Federal de Viçosa
2009-2013
Southwest Bahia State University
2012
This work aimed to optimize the production of L-asparaginase (L-ASNase) from Aspergillus caespitosus CCDCA 11593 using Pereskia aculeata (Ora-pro-nóbis) leaf fiber as a substrate for solid-state fermentation (SSF), along with powdered whey protein in submerged (SmF) processes. A centered face design was applied evaluate effect different parameters. Additionally, L-ASNase partially purified on an ion-exchange cryogel column. For SSF, experimental condition, inoculum concentration 105...
Abstract Ora‐pro‐nobis (OPN) is a plant with high protein content. The development of new adsorbents that enable the separation biomolecules while preserving their bioactivity has attracted interest researchers. This work reports functionalization cryogel via epoxy method and its application in adsorption OPN proteins through ion exchange, using amino acids. Among pH values types exchange ligands evaluated (cysteine, taurine, polyethyleneimine, glutamic acid), highest (54.44 mg g −1 ) was...
AbstractAbstractThis study aimed to produce and characterise microparticles produced from barley residue proteins (BRP) enriched with β-carotene. The were obtained by freeze-drying five emulsion formulations 0.5% w/w whey protein concentrate different concentrations of maltodextrin BRP (0, 1.5, 3.0, 4.5 6.0% w/w), the dispersed phase consisting corn oil mixtures mechanically mixed sonicated, formed emulsions freeze-drying. tested for encapsulation efficiency, humidity, hygroscopicity,...
Affinity cryogels were synthesized using tris(hydroxymethyl)aminomethane (Tris) as ligand for specific interactions with the lysozyme (LYZ). The cryogel was produced by cryo-copolymerization at −12°C. A central composite rotational design 22 used to optimize immobilization procedure of Tris on cryogel. maximum adsorption LYZ (149.5 mg/g) achieved when 376 mg/mL and 3.06 mol/L sodium borohydride during immobilization. Chromatographic separation from chicken egg white done a purity 92.13%....
In order to design and adapt equipments for food processing, it is essential know thermophysical properties. Once temperature composition affects these properties, models based in such factors are important further calculation. this work, density dynamic viscosity of bovine milk, probably the most processed fluid world, were determined within large ranges major constituents (moisture, fat, lactose, protein minerals), on typical processing values. Density varied from (962.01 1100.45) kg/m3...
Liquid–liquid equilibrium data of aqueous two phase systems (ATPS) composed polyethylene glycol (PEG) 1500 g·mol–1 + sodium sulfate water at T = (293.15, 303.15 and 313.15) K p 0.1 MPa were determined. The density the top bottom phases tie-lines (TL) was universal functional activity coefficient (UNIFAC) model correlated to experimental tie-line data, root-mean-square deviations (RMSD) between calculated considered in calculation. salting-out effect evaluated using effective volume (EEV)....
Lectins have potential for application in many fields because of their ability to selectively bind glycoconjugates. Jackfruit seeds are a source various lectins, with several isoforms and isoelectric points. Therefore, ion exchange can be viable alternative lectin separation. In this work we developed single-step purification protocol lectins from jackfruit seed extract cryogels. Supermacroporous cryogels were functionalized cationic anionic exchangers. The matrices produced showed high...
Different protocols for functionalization of cryogels with L-tryptophan were tested the lysozyme adsorption. The effect ionic strength and pH on adsorptive capacity (q) was investigated. morphological, thermal, mechanical properties determined. A maximum adsorption (58.030 mg g–1) achieved when 5% (v/v) glutaraldehyde (at 6.0) no methanol used during cryogel functionalization. hydrophobic presented a porosity 0.666. strong single band at 14.3 kDa visualized in SDS-PAGE, indicating that...