A5059939490- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Advanced Electron Microscopy Techniques and Applications
- Genomics and Phylogenetic Studies
- Viral Infections and Immunology Research
- Electron and X-Ray Spectroscopy Techniques
- Bacteriophages and microbial interactions
- Advanced biosensing and bioanalysis techniques
University College Cork
2021-2023
Programmed ribosomal frameshifting is a key event during translation of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA genome that allows synthesis viral RNA-dependent polymerase and downstream proteins. Here, we present cryo-electron microscopy structure translating mammalian ribosome primed for on RNA. The adopts pseudoknot lodges at entry to messenger (mRNA) channel generate tension in mRNA promote frameshifting, whereas nascent polyprotein forms distinct...
Abstract Our understanding of protein synthesis has been conceptualised around the structure and function bacterial ribosome. This complex macromolecular machine is target important antimicrobial drugs, an integral line defence against infectious diseases. Here, we describe how open access to cryo-electron microscopy facilities combined with bespoke user support enabled structural determination translating ribosome from Escherichia coli at 1.55 Å resolution. The obtained structures allow for...
Abstract Our understanding of protein synthesis has been conceptualised around the structure and function bacterial ribosome 1–4 . This complex macromolecular machine is target important antimicrobial drugs 5 , an integral line defence against infectious diseases. Here, we describe how open access to state-of-the-art cryogenic electron microscopy facilities combined with bespoke user support offered by newly established EMBL Imaging Centre enabled structural determination translating from...