A5021342800- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Viral Infections and Immunology Research
- Integrated Circuits and Semiconductor Failure Analysis
- SARS-CoV-2 detection and testing
- Bacteriophages and microbial interactions
- Advanced biosensing and bioanalysis techniques
- Genomics and Phylogenetic Studies
- Molecular Biology Techniques and Applications
- Advanced Electron Microscopy Techniques and Applications
- RNA Research and Splicing
- Plant Virus Research Studies
- Electrostatic Discharge in Electronics
University College Cork
2016-2023
ETH Zurich
2020-2021
Programmed ribosomal frameshifting is a key event during translation of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA genome that allows synthesis viral RNA-dependent polymerase and downstream proteins. Here, we present cryo-electron microscopy structure translating mammalian ribosome primed for on RNA. The adopts pseudoknot lodges at entry to messenger (mRNA) channel generate tension in mRNA promote frameshifting, whereas nascent polyprotein forms distinct...
Abstract Our understanding of protein synthesis has been conceptualised around the structure and function bacterial ribosome. This complex macromolecular machine is target important antimicrobial drugs, an integral line defence against infectious diseases. Here, we describe how open access to cryo-electron microscopy facilities combined with bespoke user support enabled structural determination translating ribosome from Escherichia coli at 1.55 Å resolution. The obtained structures allow for...
Abstract Programmed ribosomal frameshifting is the key event during translation of SARS-CoV-2 RNA genome allowing synthesis viral RNA-dependent polymerase and downstream proteins. Here we present cryo-EM structure mammalian ribosome in process translating paused a conformation primed for frameshifting. We observe that adopts pseudoknot lodged at mRNA entry channel to generate tension leads The nascent polyprotein being synthesized by site forms distinct interactions with polypeptide exit...
Abstract Our understanding of protein synthesis has been conceptualised around the structure and function bacterial ribosome 1–4 . This complex macromolecular machine is target important antimicrobial drugs 5 , an integral line defence against infectious diseases. Here, we describe how open access to state-of-the-art cryogenic electron microscopy facilities combined with bespoke user support offered by newly established EMBL Imaging Centre enabled structural determination translating from...