Phytophthora root and stem rot of soybean [Glycine max (L.) Merr.], caused by sojae Kaufmann Gerdemann, is a destructive disease throughout the planting regions in world. Here, we report insights into function underlying mechanisms novel ethylene response factor (ERF) soybean, namely GmERF5, host responses to P. sojae. GmERF5-overexpressing transgenic exhibited significantly enhanced resistance positively regulated expression PR10, PR1-1, PR10-1 genes. Sequence analysis suggested that GmERF5...

Isoflavone reductase (IFR) is an enzyme involved in the biosynthetic pathway of isoflavonoid phytoalexin plants. IFRs are unique to plant kingdom and considered have crucial roles response various biotic abiotic environmental stresses. Here, we report characterization a novel member soybean isoflavone gene family GmIFR. Overexpression GmIFR transgenic exhibited enhanced resistance Phytophthora sojae. Following stress treatments, was significantly induced by P. sojae, ethephon (ET), abscisic...

Phytophthora root and stem rot of soybean [Glycine max (L.) Merr.] caused by sojae is a destructive disease worldwide. Phenylalanine ammonia-lyase (PAL) one the most extensively studied enzymes related to plant responses biotic abiotic stresses. However, molecular mechanism PAL in response P. largely unclear. Here, we characterize novel member gene family, GmPAL2.1, which significantly induced sojae. Overexpression RNA interference analysis demonstrates that GmPAL2.1 enhances resistance...

Pathogenesis-related proteins (PR proteins) play crucial roles in the plant defense system. A novel PRP gene was isolated from highly resistant soybean infected with Phytophthora sojae (P. sojae) and named GmPRP (GenBank accession number: KM506762). The amino acid sequences of showed identities 74%, 73%, 72% 69% Vitis vinifera, Populus trichocarpa, Citrus sinensis Theobroma cacao, respectively. Quantitative real-time reverse transcription PCR (qRT-PCR) data that expression highest roots,...

Phytophthora root and stem rot of soybean caused by the oomycete sojae, is a destructive disease worldwide. Ethylene response factors (ERFs) play important roles in regulating plant biotic abiotic stress tolerance. In this study, new ethylene factor gene, GmERF113, was isolated from highly resistant 'Suinong 10'. Sequence analysis suggested that protein encoded GmERF113 contained conserved AP2/ERF domain 58 amino acid belonged to B-4 subgroup ERF subfamily. Expression significantly induced...

Phytophthora root and stem rot (PRR) of soybean [Glycine max (L.) Merr.] caused by the oomycete sojae, is a destructive disease worldwide. The molecular mechanism response to P. sojae largely unclear. We report novel WRKY transcription factor (TF) in soybean, GmWRKY31, host sojae. Overexpression RNA interference analysis demonstrated that GmWRKY31 enhanced resistance transgenic plants. was targeted nucleus, where it bound W-box acted as an activator gene transcription. Moreover, we...

Abstract Light‐harvesting chlorophyll a / b binding (Lhc) family proteins play significant role in photosynthetic processes. Our objective was systematic identification and analysis of the Lhc cotton, as well relationship between genes synthesis during We used genome‐wide identification, phylogenetic analysis, chromosomal distribution collinearity to examine potential functions superfamily upland cotton. Subcellular localization, qRT‐PCR, yeast two hybrid (Y2H) , Virus‐induced gene silencing...

Phytophthora root and stem rot of soybean, caused by sojae (P. sojae), is a destructive disease in many soybean planting regions worldwide. In previous study, an expressed sequence tag (EST) homolog the major allergen Pru ar 1 apricot (Prunus armeniaca) was identified up-regulated highly resistant 'Suinong 10' infected with P. sojae. Here, full length EST isolated using rapid amplification cDNA ends (RACE). It showed highest homolgy 53.46% Gly m 4 after comparison eight families reported...

Pathogenesis-related proteins, often used as molecular markers of disease resistance in plants, can enable plants to obtain systemic resistance. In this study, a gene encoding pathogenesis-related protein was identified via RNA-seq sequencing analysis performed at different stages soybean seedling development. Because the sequence showed highest similarity with PR1L soybean, named GmPR1-9-like (GmPR1L). GmPR1L either overexpressed or silenced seedlings through Agrobacterium-mediated...

Phytophthora root and stem rot, a destructive disease of soybean [Glycine max (L.) Merr.], is caused by the oomycete sojae. However, how resistance mechanisms respond to P. sojae infection remains unclear. Previously, we showed that GmWRKY31, which interacts with sucrose non-fermenting-1(SNF1)-related protein kinase (SnRK), enhances in soybean. Here, report membrane-localized SnRK GmSnRK1.1 involved host response The overexpression (GmSnRK1.1-OE) increased sojae, RNA interference...

TEOSINTE BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTOR (TCP) transcription factors are a plant-specific family and play roles in plant growth, development, responses to biotic abiotic stresses. However, little is known about the functions of TCP soybean cultivars with tolerance salt stress. In this study, TCP9-like, factor, was identified exposed The expression TCP9-like gene roots salt-tolerant higher than that salt-sensitive treated NaCl. overexpression enhanced cultivar 'DN50'. T2...

To elucidate the differential gene expression patterns in soybeans during infection by Phytophthora sojae, a cDNA library for suppression subtractive hybridization (SSH) was constructed with cDNAs from soybean cultivar Suinong 10 treated sterile distilled water as driver and inoculated P. sojae tester. A total of 2 067 recombinant colonies SSH were randomly picked, amplified, sequenced. After discarding 312 poor quality expressed sequence tags (EST), 1 755 high ESTs assembled edited to 384...

The TEOSINTE BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTOR (TCP) transcription factors is one of the superfamilies plant-specific involved in plant growth, development, and biotic abiotic stress. However, there no report on research TCP soybean response to Phytophthora sojae . In this study, Agrobacterium -mediated transformation was used introduce CRISPR/Cas9 expression vector into cultivar “Williams 82” generated targeted mutants GmTCP19L gene, which previously related involve responses P...

Abstract Time to flowering is an essential component of the adaptation and productivity cotton ( Gossipium hirsutum ) in various agro‐ecological zones. This article presents a study genetic control this trait two crosses obtained from different early‐maturity parental lines. In each cross, multiple generations including P 1 , F 2 B were evaluated under natural field conditions 2004 2005. The data on time populations had continuous distribution but deviated normality. A joint segregation...

SUMMARY Genetic manipulation of leaf architecture may be a useful breeding objective in cotton ( Gossypium spp.). The present study reports quantitative genetic analysis traits from two intraspecific crosses inbred lines upland hirsutum L.) viz. Kang3×Chaoji463 and Han109×Ji98. Six morphological (leaf area (LA), perimeter (LP), main lobe length (LL) width (LW), petiole (PL), LL/LW ratio) were recorded multiple generations (P 1 , F P 2 BC ) the crosses. Generation mean analyses conducted to...

(1) Corn is the most widely planted food crop, feed and economic crop in world, plays an important role agricultural production national economy development. The copine gene, also known as BONZAI encodes a Ca2+-dependent phospholipid membrane binding protein that present eukaryotes. It has been found negative regulator of disease resistance regulation key plants’ response. In this study, Agrobacterium-tumefacien-mediated method was used to successfully obtain T2 generation...

Ningxia Tan sheep meat is tender, has no strong smell, a delicious taste, and even fat distribution.Intramuscular (IMF) content the composition of fatty acid (FA) both are important factors associated with quality, also for tenderness flavor assessment.We examined correlation candidate genes expression these in four muscle tissues, including longissimus dorsi, triceps, biceps supraspinatus from sheep.The IMF FA different tissues were measured mRNA related (PPARγ, C/EBPα, FAS, LPL, FABP4,...

Polyamines play an important regulatory role during plant growth and development adversity stress, polyamine oxidase (PAO) is involved in catabolism. In this study, up-regulated gene GmPAO1 was obtained by transcriptome sequencing analysis screening at soybean seedling stages. Also, its expression pattern function were analyzed. The identification results of transgenic positive lines showed that the relative level overexpressed increased under salt stress. With increasing stress...

Soybean (<i>Glycine max</i> (Linn.) Merr.) annual leguminous crop is cultivated all over the world. The occurrence of diseases has a great impact on yield and quality soybean. In this study, based RNA-seq soybean variety M18, complete CDS (Coding sequence) <i>GmPR1L</i> pathogenesis-related protein 1 family was obtained, which ability to resist fungal diseases. overexpression vector interference expression were transferred into tobacco NC89, resistance transgenic (<i>Nicotiana tabacum</i>...