A5024801346- Single-cell and spatial transcriptomics
- Advanced biosensing and bioanalysis techniques
- Advanced Biosensing Techniques and Applications
- RNA and protein synthesis mechanisms
- Atmospheric and Environmental Gas Dynamics
- Monoclonal and Polyclonal Antibodies Research
- Medical Research and Treatments
- RNA Research and Splicing
- Aluminum Alloys Composites Properties
- melanin and skin pigmentation
- Congenital Anomalies and Fetal Surgery
- Respiratory viral infections research
- Methane Hydrates and Related Phenomena
- Biosensors and Analytical Detection
- Immunotherapy and Immune Responses
- Air Quality Monitoring and Forecasting
- CAR-T cell therapy research
- Hops Chemistry and Applications
- Diagnosis and treatment of tuberculosis
- Pelvic floor disorders treatments
- T-cell and B-cell Immunology
- Hydrocarbon exploration and reservoir analysis
- Gene expression and cancer classification
- Gene Regulatory Network Analysis
- Amoebic Infections and Treatments
Lanzhou University
2024
Harvard University
2021
Sun Yat-sen University
2008-2021
Arizona State University
2015-2021
Institute of Forensic Science
2010-2019
St. John Medical Center
2019
Tempe Union High School District
2015
Academy of Military Medical Sciences
2015
Sun Yat-sen Memorial Hospital
2008
First Affiliated Hospital of Xinjiang Medical University
2008
A DNA tetrahedral nanostructure-based electrochemical biosensor was developed to detect avian influenza (H7N9) virus through recognizing a fragment of the hemagglutinin gene sequence. The probe immobilized onto gold electrode surface based on self-assembly between three thiolated nucleotide sequences and longer sequence containing complementary hybridize with target single-stranded (ss)DNA. captured hybridized biotinylated-ssDNA oligonucleotide as detection probe, then avidin-horseradish...
Abstract Limitations on the number of proteins that can be quantified in single cells situ impede advances our deep understanding normal cell physiology and disease pathogenesis. Herein, we present a highly multiplexed single‐cell protein analysis approach is based chemically cleavable fluorescent antibodies. In this method, antibodies tethered to fluorophores through novel azide‐based linker are utilized detect their targets. After fluorescence imaging data storage, coupled efficiently...
A novel method to quantify the identities, positions, and copy numbers of a large number different RNA species in single cells has been developed by reiterative cycles target hybridization, fluorescence imaging photobleaching.
Understanding the composition, regulation, and function of complex biological systems requires tools that quantify multiple transcripts at their native cellular locations. However, current multiplexed RNA imaging technologies are limited by relatively low sensitivity or specificity, which hinders applications in studying highly autofluorescent tissues, such as formalin-fixed paraffin-embedded (FFPE) tissues. To address this issue, here we develop a situ profiling approach with high...
Comprehensive RNA analyses in individual cells their native spatial contexts promise to transform our understanding of normal physiology and disease pathogenesis. Here we report a single-cell situ analysis approach using switchable fluorescent oligonucleotides (SFO). In this method, transcripts are first hybridized by pre-decoding oligonucleotides. These subsequently recruit SFO stain corresponding targets. After fluorescence imaging, all the whole specimen simultaneously removed DNA strand...
The ability to comprehensively profile nucleic acids in individual cells their natural spatial contexts is essential advance our understanding of biology and medicine. Here, we report a novel method for transcriptomics genomics analysis. In this method, every acid molecule detected as fluorescent spot at its cellular location throughout the cycles consecutive fluorescence situ hybridization (C-FISH). each C-FISH cycle, oligonucleotide probes hybridize applied previous also introduce binding...
Abstract Limitations on the number of proteins that can be quantified in single cells situ impede advances our deep understanding normal cell physiology and disease pathogenesis. Herein, we present a highly multiplexed single‐cell protein analysis approach is based chemically cleavable fluorescent antibodies. In this method, antibodies tethered to fluorophores through novel azide‐based linker are utilized detect their targets. After fluorescence imaging data storage, coupled efficiently...
目的： 探讨结核杆菌特异性细胞免疫反应检测（A.TB）在尘肺并发肺结核诊断中的应用价值。 方法： 选取2013年7月至2017年12月来我院就诊的尘肺患者67人，尘肺并发肺结核患者56人；同期我院体检的健康志愿者52人分为观察组及对照组。所有受试者均同时进行A.TB检测、血清抗结核抗体检测（TB-Ab）和结核菌素皮肤（TST）试验。 结果： 尘肺并发肺结核组、尘肺组及健康对照组A.TB阳性率分别为73.21%、23.88%、13.46%。尘肺并发肺结核组、尘肺组及健康对照组TST阳性率分别为60.71%、40.29%、40.38%。尘肺并发肺结核组、尘肺组及健康对照组TB-Ab阳性率分别为35.71%、38.81%、30.77%。尘肺并发肺组的A.TB阳性率明显高于TST及TB-Ab阳性率，差异有统计学意义（P<0.05）。 结论： A.TB检测在尘肺并发结核病临床辅助诊断的应用价值明显高于TST、TB-Ab，对尘肺并发结核病具有重要的辅助诊断价值。.
Objective To provide reference for further perfection and revision of standards relevant to limb injury by comparing the evaluation results different disability long bone fracture. Methods Thirty cases were selected from fracture accepted our institution in 2018. These include 5 shoulder, elbow, wrist, hip, knee, ankle joints, respectively, investigate degree loss function joints after Disability was made according Classification Impairment Related Injury （hereinafter referred as...