A5060865245- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- HIV Research and Treatment
- Biochemical and Molecular Research
- Colorectal Cancer Screening and Detection
- Esophageal and GI Pathology
- Enzyme Structure and Function
- Enzyme function and inhibition
- Mass Spectrometry Techniques and Applications
- Bacteriophages and microbial interactions
- Amino Acid Enzymes and Metabolism
- Urologic and reproductive health conditions
- Protein Structure and Dynamics
- vaccines and immunoinformatics approaches
- Celiac Disease Research and Management
- HIV/AIDS drug development and treatment
- Cell Adhesion Molecules Research
- CAR-T cell therapy research
- Gastric Cancer Management and Outcomes
- Microscopic Colitis
- T-cell and Retrovirus Studies
- Cancer Research and Treatments
- Nuclear Structure and Function
Indiana University – Purdue University Indianapolis
2021-2025
Indiana University School of Medicine
2022-2025
Indiana University
2024
University of Indianapolis
2024
Merrion Fertility Clinic
2023
University of Louisville
2014-2021
National Institutes of Health
2013-2018
National Institute of Environmental Health Sciences
2013
Laboratory of Molecular Genetics
2013
Institute of Genetics and Biophysics
1997-2008
INTRODUCTION: Data on the risk of post-polypectomy bleeding after endoscopic mucosal resection (EMR) in patients with cirrhosis are limited. METHODS: This retrospective cohort study used US Collaborative Network to assess colorectal EMR compared controls. Using one-to-one propensity score matching, primary outcome measured was within 30 days post-EMR. The further stratified by severity (compensated and decompensated). RESULTS: After each included 2,381 patients. Patients had a higher (odds...
Previous studies indicated that CD3-CD16+ natural killer (NK) cells are capable of specific alloantigen recognition. Thus, alloreactive NK clones lysed normal allogeneic target (phytohemagglutinin [PHA] blasts) bearing the stimulating but did not lyse autologous or majority unrelated cells. In this study we investigated whether isolated from single individuals could exhibit different allospecificities. To end, derived large numbers (in presence PHA) fresh CD3- peripheral blood lymphocytes....
Abstract Long-term persistence of Ag-experienced CD8 cells, a class T lymphocytes with cytotoxic function, contributes to immunological memory against intracellular pathogens. After Ag clearance, cells are maintained over time by slow proliferation, primarily cytokine driven. In this article, we show that the bone marrow (BM) is crucial organ where such basal division occurs. BM contain higher percentage proliferating than their corresponding in either spleen or lymph nodes from C57BL/6...
We analyzed the recently defined ability of CD3-CD16+ cells to specifically recognize and lyse normal allogeneic target (PHA-induced blasts). The susceptibility lysis by a given alloreactive natural killer (NK) clone ("1 anti-A") was expressed PHA blasts derived from 9 38 random donors analyzed. In all instances, specific "susceptible" greater than 35% while that "nonsusceptible" targets less 6% at an E/T cell ratio 5:1. addition 1 anti-A, A anti-1 clones could also be isolated reverse MLC...
Abstract To study naive and memory CD8 T cell turnover, we performed BrdU incorporation experiments in adult thymectomized C57BL/6 mice analyzed data a mathematical framework. The following aspects were novel: 1) examined the bone marrow, addition to spleen lymph nodes, took into account sum of cells contained three organs; 2) describe both BrdU-labeling -delabeling phase, designed general model, which populations distinguished based on number divisions; 3) find parameters, used...
Evidence is reported that shows the presence in Escherichia coli K-12 of a newly found acetolactate synthase. This enzyme product two genes, ilvH and ilvI , both located very close to leu . Amber mutations have been genes therefore their products are polypeptides. Mutations gene cause appearance an synthase activity which relatively resistant valine inhibition can be separated by adsorption on hydroxylapatite from another present extract more sensitive than former. A mutant altered was...
The kinetics of isoleucine, leucine, and valine transport in Escherichia coli K-12 has been analyzed as a function substrate concentration. Such analysis permits an operational definition several systems having different affinities for their substrates. identification these was made possible by experiments on specific mutants whose isolation characterization is described elsewhere. process with highest affinity called the “very-high-affinity”process. Isoleucine, are substrates this apparent...
Raji, a human B-lymphoma cell line, expresses high levels of class II (Ia) antigens. The expression Ia antigens is totally abolished at the level specific mRNA accumulation in RJ 2.2.5, variant line derived from Raji after mutagenesis and immunoselection. We report here that antigen can be restored interspecies somatic hybrids between 2.2.5 cells BALB/c-derived M12.4.1 (I-A+, I-E+) cells. Two hybrid clones were studied detail. In both molecules DR NG2 type easily detected by surface...
Raji, a human B lymphoma line, expresses high levels of major histocompatibility complex (MHC) class II antigens. Conversely, none the detectable Ia antigens is present in RJ 2.2.5, an immunoselected Raji variant. Clonal analysis, biochemical characterization, and nucleic acid hybridization studies hybrids between mouse spleen cells 2.2.5 show that MHC gene expression regulated trans by factor which, as judged dominance studies, has characteristics activator. Such positive acting expressed...
Two mutants of Escherichia coli K-12, defective in the oligopeptide and dipeptide transport system, are described. A mutant system ( opp-1 ) was isolated as resistant to inhibitory action triornithine; this is also glycylglycylvaline does not concentrate 14 C-glycylglycylglycine, although it still sensitive parental strain glycylvaline valine. Starting from strain, a dpp-1 isolated; glycylvaline, valylleucine, leucylvaline C-glycylglycine, The apparent kinetic constants for were measured....
Cancer and infection are predominant causes of human mortality derive, respectively, from inadequate genomic host defenses against environmental agents. The transcription factor p53 plays a central role in tumor suppression. Despite its expression immune cells broad responsiveness to stressors, it is virtually unknown whether regulates defense infection. We report that the lungs naive p53−/− mice display genome-wide induction NF-κB response element–enriched proinflammatory genes, suggestive...
We previously isolated a CD4 ligand glycoprotein, gp17, from human seminal plasma; this glycoprotein is identical with gross cystic disease fluid protein-15 (GCDFP-15), factor specifically secreted primary and secondary breast tumors. The function of gp17/GCDFP-15 in physiological as well pathological conditions has remained elusive thus far. As follow up to our previous findings that gp17 binds high affinity interferes both HIV-1 gp120 binding syncytium formation, we investigated whether...
Virions of filamentous bacteriophage fd are capable displaying multiple copies peptide epitopes and generating powerful immune responses to them. To investigate the antigen processing mechanisms in human B cell lines used as presenting cells, major coat protein (pVIII) intact virions was fluorescently labeled, its localization various intracellular compartments followed using confocal microscopy. We show that were taken up processed yield peptides reach both histocompatibility complex (MHC)...
The specificity recognized on normal allogeneic cells by a given alloreactive (1-anti-A) natural killer clone is controlled gene locus termed EC1. Because the EC1 was previously shown to be located chromosome 6, families characterized recombinant major histocompatibility complex haplotype were analyzed map this more precisely. breakpoint of recombination studied standard HLA typing, complement and restriction fragment length polymorphism analysis series genes between cluster HLA-B within...
Two mutants of Escherichia coli K-12 are described which resistant to the inhibition that valine exerts on growth E. . These have lesions at two different loci chromosome. One them, brnP , is linked leu (87% cotransduction) and located between azi represented map 1 min; other, brnQ phoA (96% cotransduction), probably proC 10 min. but sensitive dipeptides containing valine. Since it known taken up by through a transport system(s) from those used amino acids, this sensitivity peptides suggests...