A5100389029- Hemoglobin structure and function
- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Heme Oxygenase-1 and Carbon Monoxide
- Neonatal Health and Biochemistry
- Cancer Cells and Metastasis
- Erythrocyte Function and Pathophysiology
- Microfluidic and Bio-sensing Technologies
- RNA Interference and Gene Delivery
- Spaceflight effects on biology
- Microtubule and mitosis dynamics
- Curcumin's Biomedical Applications
- Advanced NMR Techniques and Applications
- Aldose Reductase and Taurine
- Tendon Structure and Treatment
- Tissue Engineering and Regenerative Medicine
- Extracellular vesicles in disease
- Neuroscience and Neural Engineering
- Hemoglobinopathies and Related Disorders
- Blood properties and coagulation
- Advanced biosensing and bioanalysis techniques
- Wound Healing and Treatments
- Lipid Membrane Structure and Behavior
- Aluminum toxicity and tolerance in plants and animals
Changzhou University
2020-2025
University of Bergen
2020
Tiangong University
2008-2018
Peking University
2012
<title>Abstract</title> Molecular dynamics (MD) simulations struggle to resolve rare conformational transitions hindered by high energy barriers. We introduce the Equivalent Weight Method (EWM), an enhanced sampling framework that combines dynamic trajectory segmentation and adaptive weight balancing guided real-time density analysis. EWM enables comprehensive exploration strategically combining multiple low-dimensional collective variable (CV) spaces with CV augmentation. Validated across...
Tunneling nanotubes (TNTs) are thin membrane tubular structures that interconnect physically separated cells. Growing evidence indicates TNTs play unique roles in various diseases by facilitating intercellular transfer of signaling and organelles, suggesting as a potential target for disease treatment. The efficiency TNT-dependent communication is largely determined the number between Though fragile structures, mechanical properties determinants their stability still unclear. Here, using...
Circulating tumor cells (CTCs) with different epithelial and mesenchymal phenotypes play distinct roles in the metastatic cascade. However, influence of their phenotypic traits chemotherapy on transit retention within capillaries remains unclear. To explore this, we developed a microfluidic device comprising 216 microchannels widths from 5 to 16 μm mimic capillaries. This platform allowed us study behaviors human breast cancer MCF-7 MDA-MB-231 through under chemotherapy-induced stress. Our...
Significance Tissue development and remodeling are highly regulated by the forces from cell contraction collagen cross-linking, which is also dependent on microenvironment mechanics. However, such have seldom been studied in very soft tissue because of a lack techniques to detect force without interference probe. Here, we overcame this challenge using polymer-based, flexible microstrings grow microtissues then quantify strain-induced measuring microstring deflection during large-scale...
Red blood cells (RBCs) possess intact cyto-architectures while haemoglobin (Hb) is a cell-free, homogeneous solution. Both RBCs and Hb are generalized oxygen carriers. In this paper, kinetic studies on oxygen-releasing of high concentration under various conditions were carried out regarding as fluids. Among them, specific was seen the simplest Hb-based carrier (HBOC), Also, factors affecting releasing RBCs, including osmotic pressure, viscosity allosteric agent, have been well studied....
Increasing the size of hemoglobin (Hb) by polymerization offers benefits reduced renal clearance and increased duration in vascular circulation. With this goal, diaspirin cross-linked (DCLHb) was modified order to keep one thiol group on surface then polymerized with 1,6-bismaleimic-hexane (1,6-BMH) increase molecular weight. The HPLC results indicated that approximate 20% dimers tetramers DCLHb desired were achieved after polymerization. It also demonstrated oxygen-carrying capacity...
Soft tissues experience strain under mechanical stresses, storing energy as residual stresses and energy. However, the specific impact of such on cell migration its molecular mechanisms remains unclear. In this study, we investigated by using polydimethylsiloxane (PDMS) membranes with varying prestrain levels but constant stiffness to mimic tissue-like conditions. Results showed that higher enhanced 3T3 fibroblast adhesion reduced filopodia formation. Elevated also increased integrin...
There is a worldwide increased demand for blood transfusions.In Third World countries not sufficient due to the spreading of infections, such as HIV.The current generation so-called hemoglobin-based oxygen carriers (HBOCs) comprise hemoglobins from different species (human and bovine) that have been chemically modified improve structural stability.However, some adverse effects hypertension, myocardial infarction acute kidney failure found in several clinical trials.The exact factors leading...
Hemoglobin-based blood substitute is widely studied. As the starting materials, hemoglobin (Hb) mainly supplied from outdated human and animal sources. But there are many disadvantages. alternative source, placenta (PHb) has its own advantages. We take lead in being occupied research of substitutes based on blood. Purified PHb readily undergoes dissociation to form two dimers that easily filtered by kidneys have high 02 affinity (low P50), failing transport tissues. a first step, enhance...
With the great progress of hemoglobin-based blood substitutes,however, theyallhave a same problem that is hard to get effective stable structure hemoglobin.The hemoglobin can be oxidized into met hemoglobinin process purification,lead loss function carrying and transporting oxygen.The combination CO placenta ability far higher than O2, properties carbon monoxide (HbCO) has more stable.Red cell (RBC)is mixed with organic solvent for hemolysis centrifuged removal stroma.To prevent MetHb...
Cell-generated contraction force is the primary physical drive for fibrotic densification of biological tissues. Previous studies using two-dimensional culture models have shown that epithelial cells inhibit myofibroblast-derived via regulation fibroblast/myofibroblast transition (FMT). However, it remains unclear how interact with fibroblasts and myofibroblasts to determine mechanical consequences spatiotemporal fibrosis development. In this study, we established a three-dimensional...