A5050664803- Immune cells in cancer
- Immune Cell Function and Interaction
- Histone Deacetylase Inhibitors Research
- Cellular transport and secretion
- Peptidase Inhibition and Analysis
- Cancer Immunotherapy and Biomarkers
- Autophagy in Disease and Therapy
- Circular RNAs in diseases
- Endoplasmic Reticulum Stress and Disease
- MicroRNA in disease regulation
- Extracellular vesicles in disease
- Genetic Neurodegenerative Diseases
- RNA and protein synthesis mechanisms
- Hereditary Neurological Disorders
- Hippo pathway signaling and YAP/TAZ
- Phagocytosis and Immune Regulation
- RNA Research and Splicing
- Neurobiology and Insect Physiology Research
- NF-κB Signaling Pathways
- Signaling Pathways in Disease
- Lysosomal Storage Disorders Research
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Ion Channels and Receptors
- Mitochondrial Function and Pathology
Tianjin Medical University Cancer Institute and Hospital
2020-2025
Tianjin Medical University
2020-2024
Nankai University
2019-2020
Institute of Biophysics
2019
Chinese Academy of Sciences
2019
TNFR2+ regulatory T cells preferentially accumulate in the tumor microenvironment, express high levels of immunosuppressive molecules and possess strong suppressive activity. Our study aimed to explore characteristics role Tregs microenvironment progression gastric cancer via polychromatic immunofluorescence, single-cell RNA sequencing flow cytometry assays. The Treg infiltration level increased significantly as progressed was demonstrated be a prognostic marker. Single-cell revealed TNFR2...
Significance In the early secretory pathway, newly synthesized proteins undergo folding and modifications then leave ER through COPII-coated vesicles. How these processes are coordinated maintained important but mostly unclear. We show here that ATL, a GTPase connects tubules, controls protein mobility regulates cargo packaging coat assembly of COPII The tethering fusion activity by ATL likely maintains tension other necessary parameters for formation in membranes. These findings reveal role...
Abstract Endoplasmic reticulum (ER) is characterized by interconnected tubules and sheets. Neuronal ER adopts specific morphology in axons, dendrites soma. Here we study mechanisms underlying morphogenesis a C. elegans sensory neuron PVD. In PVD soma dendrite branch points, connect to form networks. fill primary but only extend some not all dendritic branches. We find that the Atlastin-1 ortholog, atln-1 required for neuronal morphology. mutants with impaired GTPase activity, networks points...
Aberrant expression of histone deacetylase 6 (HDAC6) is greatly involved in neoplasm metastasis, which a leading cause colon cancer related death. Thus, deep understanding the regulatory mechanisms HDAC6 metastasis warranted. In this study, we firstly found that was highly expressed metastatic tissues and inhibition or knockdown suppressed metastasis. Next, based on proteomic analysis uncovered A-kinase anchoring protein 12 (AKAP12) novel substrate HDAC6. interacted with AKAP12 deacetylated...
Circular RNA (circRNA) is a novel molecule characterized by covalently closed loop structure. Since its discovery, researchers have shown that circRNA not "splicing noise" but participant of various pathophysiological processes through unique mechanisms. circIPO7, which was identified as an independent prognostic factor in gastric cancer (GC) patients, downregulated GC tissues and cells compared to paracarcinoma normal epithelial cells. circIPO7 overexpression significantly suppressed cell...
Tumor necrosis factor receptor type 2 (TNFR2) is primarily expressed by CD4+FoxP3+ regulatory T cells (Tregs), especially those present in tumor microenvironment. There compelling evidence that TNFR2 plays a crucial role the activation, expansion, and phenotypic stability of Tregs promotes immune evasion. Understanding epigenetic regulation expression may help device novel strategy cancer immunotherapy.MiR-125b-5p-overexpressing or knockdown murine CD4 were constructed, effect miR-125b-5p on...
Abstract Histone deacetylase 6 (HDAC6), a member of the HDAC family, has been identified as potential therapeutic target for tumor therapy, but function and underlying mechanisms HDAC6 in colon cancer are incompletely characterized. Our study showed that infiltration ratio M2 macrophages was increased tissues with high expression. Similarly, knockdown cells inhibited cocultured macrophage polarization vitro. Analysis antibody chip revealed promoted sIL-6R release to enhance polarization....
Background Tumor-infiltrating regulatory T cells (TI-Tregs) are well-adapted to thrive in the challenging tumor microenvironment (TME) by undergoing metabolic reprogramming, notably shifting from glycolysis mitochondrial oxidative phosphorylation (OXPHOS) for energy production. The extracellular matrix is an important component of TME, contributing regulation both and immune cell metabolism patterns activating mechanosensors such as YAP. Whether YAP plays a part regulating TI-Treg function...
Store-operated Ca2+ entry (SOCE) is critical for numerous Ca2+-related processes. The activation of SOCE requires engagement between stromal interaction molecule 1 (STIM1) molecules on the endoplasmic reticulum and release-activated channel (CRAC) Orai plasma membrane. However, molecular details their interactions remain elusive. Here, we analyzed STIM1-Orai using synthetic peptides derived from N- C-termini channels (Orai-NT Orai-CT, respectively) purified fragments STIM1. binding STIM1 to...
Circular RNAs (circRNAs) are a novel class of endogenous noncoding (ncRNAs) with covalently closed loop structure. Accumulating evidence shows that circRNAs play vital roles in the growth, metastasis, treatment and prognosis various cancers. However, detailed functions underlying mechanisms circEVI5 (hsa_circ_0013162) gastric cancer (GC) remain undocumented. In this study, expression levels prognostic value were validated GC tissue samples by using qRT-PCR. was significantly downregulated...
In eukaryotic cells, the endoplasmic reticulum (ER) forms a continuous network of tubules and sheets. ER membranes are inter-connected by class dynamin-like GTPases termed atlastins (ATLs). Deletion or mutation ATLs results in long unbranched cells. Mutations ATL1 humans have been linked to neurodegenerative disease hereditary spastic paraplegia. The basis ATL-mediated membrane fusion has studied extensively, but specific functions ATL remain unclear. this review, we summarize ER-related...
Abstract circIPO7 dissociates caprin-1 from ribosomes and inhibits gastric cancer cell proliferation by suppressing EGFR mTORLiling Niu, Jing Liu, Hui Li*Purpose: Circular RNAs (circRNAs) are vital participant of various pathophysiological processes through unique mechanisms. We previously reported that (circBase ID: hsa_-circ_0005092) was positively associated with postoperative recurrence (GC). In the present study, we aimed to explore biological function in GC development. Methods: CCK-8,...
Abstract BACKGROUND & AIMS: Regulatory T cells (Tregs) are potent immunosuppressive of the immune system. It is reported that expression TNFR2 identifies a subpopulation Tregs with maximally suppressive function. Thus, understanding heterogeneity human CD4+FOXP3+ and function TNFR2+ critical importance for moving Treg therapy into clinics. METHODS: The phenotype from gastric cancer patients normal controls were detected by flow cytometry. Further, we quantified infiltrated Foxp3+ using...
Abstract Histone deacetylase 6 (HDAC6), a member of the HDAC family, has been identified as potential therapeutic target for tumor therapy, but function and underlying mechanisms HDAC6 in colon cancer are incompletely characterized. Our study showed that infiltration ratio M2 macrophages was increased tissues with high expression. Similarly, knockdown cells inhibited co-cultured macrophage polarization vitro. Analysis antibody chip revealed promoted sIL-6R release to enhance polarization....