Anaerobic ammonium oxidation coupled to iron(III) reduction, termed Feammox, is a newly discovered nitrogen cycling process. However, little known about the roles of electron shuttles in Feammox reactions. In this study, two forms Fe(III) (oxyhydr)oxide ferrihydrite (ex situ and ferrihydrite) were used dissimilatory reduction (DIR) enrichments from paddy soil. Evidence for DIR was demonstrated using 15N-isotope tracing technique. The extent rate both 30N2–29N2 Fe(II) formation enhanced when...

A Gram-stain-negative and rod-shaped bacterial strain, 12-OD1T, with rock phosphate solubilizing ability was isolated from agricultural soil in Hailun, Heilongjiang, PR China. The isolate affiliated to the genus Massilia, based on 16S rRNA gene sequence alignments, having highest similarities Massilia putida6 NM-7T (98.67 %), kyonggiensis TSA1T (98.28 norwichensis NS9T (98.07 respectively. DNA G+C content 67.72 mol% DNA-DNA hybridization showed low relatedness values (less than 47 %) between...

The ability to solubilize fixed inorganic phosphorus (P) for plant growth is important increasing crop yield. More P can be released by inoculating soil with inorganic-phosphate-solubilizing bacteria (iPSBs). We used 96-well microplates instead of traditional 200-mm petri dishes rapidly screen iPSB strains their solubilizing ability. simultaneously obtained 76 isolates from 576 wells containing two agricultural soils. This method conveniently identified positive and effectively prevented...

Anaerobic ammonium oxidation coupled to iron reduction (Feammox) is a recently discovered pathway contributing nitrogen loss in various ecosystems such as paddy soils and sediments. However, little known about the microbes driving Feammox an agricultural ecosystem. Here, we demonstrated occurrence of Southern China using 15N isotopic tracing technique, examined microbial communities associated with RNA based stable isotope probing (RNA-SIP) combined Illumina sequencing. was detected all...

Abstract Inorganic phosphate solubilizing bacteria (iPSB) are essential to facilitate phosphorus (P) mobilization in alkaline soil, however, the phylogenetic structure of iPSB communities remains poorly characterized. Thus, we use a reference database analyze distribution based on 16S rRNA gene illumina sequencing. Additionally, noval pqqC primer was developed quantify abundance. In our study, an soil with 27-year fertilization treatment selected. The percentage 1.10~2.87% per sample, and...

Glass sponge (Hexactinellida, Porifera) is a special lineage because of its unique tissue organization and skeleton material. Structure physiology glass have been extensively studied. However, our knowledge the sponge-associated microbial community interaction with host rather limited. Here, we performed genomic studies on in Lophophysema eversa seamount. The was dominated by an ammonia-oxidizing archaeum (AOA), nitrite-oxidizing bacterium (NOB) sulfur-oxidizing (SOB), all which were...

Microbe-mediated biogeochemical cycle of nitrogen is a critical process in the environment. In this study, surface-enhanced Raman spectroscopy combined with 15N stable isotope probing (SERS–15N SIP) was developed as new, nondestructive, and robust approach to probe assimilation by bacteria at both bulk single-cell level, from pure culture environmental microbial community. Multiple distinguishable SERS band shifts were observed displayed linear relationship content, because substitution...

Diets of soil-feeding earthworms contain abundant nitrate and iron(III) oxides, which are potential electron acceptors for mineralization organic compounds. The earthworm gut provides an ideal habitat ingested iron(III)-reducing microorganisms. However, little is known about reduction its interaction with other processes in the guts earthworms. Here, we determined dynamics revealed turnover acids Pheretima guillelmi. Samples from contents combined anoxic incubation were used chemical...

Soil cadmium (Cd) pollution presents a severe burden to flora and fauna due its non-degradability transferability. The Cd in the soil is stressing silkworm (Bombyx mori) out through soil-mulberry-silkworm system. gut microbiota of B.mori are reported shape host health. However, earlier research had not effect endogenous Cd-polluted mulberry leaves on B.mori. In current research, we compared phyllosphere bacteria at different concentrations. investigation fed with was done evaluate impact Cd-...

Abstract Significant amounts of organic carbon in marine sediments are degraded, coupled with sulfate reduction. However, the actual and energy sources used situ have not been assigned to each group diverse sulfate-reducing microorganisms (SRM) owing microbial environmental complexity sediments. Here, we probed activity temperate permanently cold by using potential SRM substrates, fermentation products at very low concentrations (15–30 μM), RNA-based stable isotope probing. Unexpectedly,...

Abstract Asgard is a recently discovered archaeal superphylum, closely linked to the emergence of eukaryotes. Among archaea, Lokiarchaeota are abundant in marine sediments, but their situ activities largely unknown except for Candidatus ‘Prometheoarchaeum syntrophicum’. Here, we tracked activity incubations with Helgoland mud area sediments (North Sea) by stable isotope probing (SIP) organic polymers, 13C-labelled inorganic carbon, fermentation intermediates and proteins. Within active...

Abstract Metagenomic analysis has facilitated prediction of a variety carbon utilization potentials by uncultivated archaea including degradation protein, which is wide-spread polymer in marine sediments. However, the activity detrital catabolic protein mostly unknown for vast majority archaea. Here, we show actively executed catabolism three archaeal phyla (uncultivated Thermoplasmata, SG8-5; Bathyarchaeota subgroup 15; Lokiarchaeota 2c) RNA- and lipid-stable isotope probing incubations...

Biochar contains quinones and aromatic structures that facilitate extracellular electron transfer between microbial cells insoluble minerals. In this study, granulated biochar (1.2-2 mm) powdered (<0.15 were amended to two ferrihydrite (

Abstract Background The trophic strategy is one key principle to categorize microbial lifestyles, by broadly classifying microorganisms based on the combination of their preferred carbon sources, electron and sinks. Recently, a novel strategy, i.e., chemoorganoautotrophy—the utilization organic as energy source but inorganic sole source—has been specifically proposed for anaerobic methane oxidizing archaea (ANME-1) Bathyarchaeota subgroup 8 (Bathy-8). Results To further explore...

Resistomes are ubiquitous in natural environments. Previous studies have shown that both the plant phyllosphere and soil-borne nematodes were reservoirs of above- below-ground resistomes, respectively. However, influence identity on soil, nematode, resistomes remains unclear. Here, a microcosm experiment was used to explore characteristics bacterial communities associated with six different identities (Lactuca sativa, Cichorium endivia, Allium fistulosum, Coriandrum sativum, Raphanus...

A novel 2-D heterometal-organic coordination polymer [AgCu2(C7H2NO5)(C7H3NO5)]n·2nH2O, obtained by a hydrothermal reaction, exhibits (44) topology and good third-order nonlinear optical magnetic properties, which may be candidate for NLO multifunctional materials.

The biology of biofilm in deep-sea environments is barely being explored. Here, biofilms were developed at the brine pool (characterized by limited carbon sources) and normal bottom water adjacent to Thuwal cold seeps. Comparative metagenomics based on 50 Gb datasets identified polysaccharide degradation, nitrate reduction proteolysis as enriched functional categories for biofilms. genomes two dominant species: a novel Deltaproteobacterium Epsilonproteobacterium reconstructed. Despite rather...

Abstract Anaerobic methanotrophic archaea (ANME) can assimilate methane and govern the greenhouse effect of deep-sea cold seeps. In this study, a total 13 ANME draft genomes representing five types (ANME-1a, ANME-1b, ANME-2a, ANME-2b ANME-2c), in size between 0.8 1.8 Mbp, were obtained from Jiaolong seep South China Sea. The small metagenome-assembled (MAGs) contained all essential pathways for oxidization carbon dioxide fixation. All genes related to nitrate sulfate reduction absent MAGs,...

Microplastics (MPs) have garnered global attention as emerging contaminants due to their adaptability, durability, and robustness in various ecosystems. Still, studies concerning combination with heavy metals (HMs), interactions soil biota, how they affect physiochemical properties terrestrial plant systems are limited. Our study was set investigate the combined effect of HMs (cadmium, arsenic, copper, zinc lead) contaminated Tongling different sizes (T1 = 106 µm, T2 50 T3 13 µm) polystyrene...

Three coordination polymers, [Ag4(L1)2(NO3)4]·H2O (1), [Ag(L1)](BF4)·2H2O (2) and [Ag2(L1)(H2O)](ClO4)2 (3), were obtained from self-assembly of AgX (X = NO3−, BF4−, or ClO4−) with a new flexible double betaine L1 (L1 1,3-bis(pyridinio-4-carboxylato)propane). Single crystal X-ray diffraction studies for complexes 1–3 reveal layered open network structures containing novel cavities channels. Complex 1 consists an unprecedented rhombic tetrameric Ag4O2 subunit, in which μ3-η1 mode the...

Anaerobic ammonium oxidation coupled to nitrite reduction (termed as Anammox) was demonstrated an efficient pathway remove nitrogen from a wastewater treatment system. Recently, anaerobic also identified be linked iron(III) Feammox) with dinitrogen, nitrite, or nitrate end-product, reporting enhance removal the However, little is known about role of Anammox bacteria in Feammox process. Here, slurry reactor amended ferrihydrite employed investigate activity process using 15 N isotopic tracing...

A new series of novel isostructural metal chalcogenides, K(2)CuIn(3)Se(6) (1), K(2)CuGa(3)Se(6) (2), and K(2)AgIn(3)Se(6) (3), were obtained by a reactive flux technique structurally characterized. Compounds 1, 2, 3 crystallize in the space group C2/c monoclinic system with eight formula units cell: = 11.445(2) A, b 11.495(2) c 21.263(4) beta 97.68(3) degrees, V 2772(1) A(3), R1/wR2 0.0676/0.1652 for 1; 11.031(2) 11.050(4) 20.808(7) 97.71(2) 2513(1) 0.0301/0.0511 2; 11.633(1) 11.587(1)...