A5091566874- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- Methane Hydrates and Related Phenomena
- Anaerobic Digestion and Biogas Production
- Microbial Metabolic Engineering and Bioproduction
- Biofuel production and bioconversion
- Bacteriophages and microbial interactions
- Gut microbiota and health
- Wastewater Treatment and Nitrogen Removal
- Coastal wetland ecosystem dynamics
- Microbial Fuel Cells and Bioremediation
- Photosynthetic Processes and Mechanisms
- Sphingolipid Metabolism and Signaling
- Probiotics and Fermented Foods
- Tracheal and airway disorders
- Biochemical and Molecular Research
- Advanced Sensor and Control Systems
- Photodynamic Therapy Research Studies
- Bioactive natural compounds
- Microbial Natural Products and Biosynthesis
- Environmental DNA in Biodiversity Studies
- Mercury impact and mitigation studies
- Synthesis and Biological Activity
- Control Systems in Engineering
- Lysosomal Storage Disorders Research
Shenzhen University
2018-2024
Shenzhen Bay Laboratory
2021-2024
Minzu University of China
2024
City University of Hong Kong
2015-2018
Xi'an University of Science and Technology
2010-2011
Anaerobic digestion (AD) is a microbial process widely used to treat organic wastes. While the microbes involved in of municipal sludge are increasingly well characterized, taxonomic and functional compositions AD digesters treating industrial wastewater have been understudied. This study examined metagenomes from biogas-producing digester Shek Wu Hui (SWH), Hong Kong an Guangzhou (GZ), China, compared their composition reconstructed biochemical pathways. Genes encoding carbohydrate...
Hadal biosphere represents the deepest part of ocean with water depth >6,000 m. Accumulating evidence suggests existence unique microbial communities dominated by heterotrophic processes in this environment. However, investigations diversity and their metabolic potentials are limited because technical constraints for sample collection. Here, we provide a detailed metagenomic analysis three seawater samples at depths 5,000-6,000 m below sea level (mbsl) surface sediment 4,435-6,578 mbsl Yap...
Bathyarchaeota, a newly proposed archaeal phylum, is considered as an important driver of the global carbon cycle. However, due to great diversity them, there limited genomic information that accurately encompasses metabolic potential entire phylum.In current study, nine metagenome-assembled genomes Bathyarchaeota from four subgroups were constructed mangrove sediments, and metatranscriptomes obtained for evaluating their in situ transcriptional activities. Comparative analyses with...
There is increasing awareness that archaea are interrelated with human diseases (including cancer). Archaea utilize unique metabolic pathways to produce a variety of metabolites serve as direct link host-microbe interactions. However, knowledge on the diversity human-associated still extremely limited, and less known about pathological effects their tumor microenvironment carcinogenesis. In present study, we performed large-scale analysis cancer-related across different body sites using...
Sulfonolipids (SoLs) are a unique class of sphingolipids featuring sulfonate group compared to other sphingolipids. However, the biological functions and biosynthesis SoLs in human microbiota have been poorly understood. Here, we report discovery isolation from opportunistic pathogen Chryseobacterium gleum DSM16776. We show for first time pro-inflammatory activity with mice primary macrophages. Furthermore, used both vivo heterologous expression vitro biochemical reconstitution characterize...
Plitidepsin (or dehydrodidemnin B), an approved anticancer drug, belongs to the didemnin family of cyclic depsipeptides, which are found in limited quantities marine tunicate extracts. Herein, we introduce a new approach that integrates microbial and chemical synthesis generate plitidepsin its analogues. We screened Tistrella strain library identify potent B producer, then introduced second copy biosynthetic gene cluster into genome, resulting titer approximately 75 mg/L. Next, developed two...
Abstract Asgard is a recently discovered archaeal superphylum, closely linked to the emergence of eukaryotes. Among archaea, Lokiarchaeota are abundant in marine sediments, but their situ activities largely unknown except for Candidatus ‘Prometheoarchaeum syntrophicum’. Here, we tracked activity incubations with Helgoland mud area sediments (North Sea) by stable isotope probing (SIP) organic polymers, 13C-labelled inorganic carbon, fermentation intermediates and proteins. Within active...
Abstract Metagenomic analysis has facilitated prediction of a variety carbon utilization potentials by uncultivated archaea including degradation protein, which is wide-spread polymer in marine sediments. However, the activity detrital catabolic protein mostly unknown for vast majority archaea. Here, we show actively executed catabolism three archaeal phyla (uncultivated Thermoplasmata, SG8-5; Bathyarchaeota subgroup 15; Lokiarchaeota 2c) RNA- and lipid-stable isotope probing incubations...
Although anaerobic digestion for biogas production is used worldwide in treatment processes to recover energy from carbon-rich waste such as cellulosic biomass, the activities and interactions among microbial populations that perform deserve further investigations, especially at population genome level. To understand biomass-degrading potentials two full-scale digesters, this study examined five methanogenic enrichment cultures derived digesters anaerobically digested cellulose or xylan more...
Bathyarchaeia represent a class of archaea common and abundant in sedimentary ecosystems. Here we report 56 metagenome-assembled genomes viruses identified metagenomes from different environments. Gene sharing network phylogenomic analyses led to the proposal four virus families, including realms Duplodnaviria Adnaviria, archaea-specific spindle-shaped viruses. Genomic uncovered diverse CRISPR elements these Viruses proposed family "Fuxiviridae" harbor an atypical Type IV-B CRISPR-Cas system...
Abstract Background The trophic strategy is one key principle to categorize microbial lifestyles, by broadly classifying microorganisms based on the combination of their preferred carbon sources, electron and sinks. Recently, a novel strategy, i.e., chemoorganoautotrophy—the utilization organic as energy source but inorganic sole source—has been specifically proposed for anaerobic methane oxidizing archaea (ANME-1) Bathyarchaeota subgroup 8 (Bathy-8). Results To further explore...
Cellulose and xylan are two major components of lignocellulosic biomass, which represents a potentially important energy source, as it is abundant can be converted to methane by microbial action. However, recalcitrant hydrolysis, the establishment complete anaerobic digestion system requires specific repertoire functions. In this study, we maintained 2-year enrichment cultures sludge amended with cellulose or investigate whether cellulose- xylan-digesting could assembled from previously used...
ABSTRACT Coculturing dark- and photofermentative bacteria is a promising strategy for enhanced hydrogen (H 2 ) production. In this study, next-generation sequencing was used to query the global transcriptomic responses of an artificial coculture Clostridium cellulovorans 743B Rhodopseudomonas palustris CGA009. By analyzing differentially regulated gene expression, we showed that, consistent with physiological observations H production cellulose degradation, nitrogen fixation genes in R....
Abstract Plitidepsin (or dehydrodidemnin B), an approved anticancer drug, belongs to the didemnin family of cyclic depsipeptides, which are found in limited quantities marine tunicate extracts. Herein, we introduce a new approach that integrates microbial and chemical synthesis generate plitidepsin its analogues. We screened Tistrella strain library identify potent B producer, then introduced second copy biosynthetic gene cluster into genome, resulting titer approximately 75 mg/L. Next,...
Abstract Background Amplification of small subunit (SSU) rRNA genes with universal primers is a common method used to assess microbial populations in various environmental samples. However, owing limitations coverage these primers, some microorganisms remain unidentified. The present study aimed establish for amplifying nearly full-length SSU gene sequences previously unidentified prokaryotes, using newly designed targeted via primer evaluation meta-transcriptomic datasets. Methods Primer...