A5075205918- Nitric Oxide and Endothelin Effects
- Glutathione Transferases and Polymorphisms
- Mitochondrial Function and Pathology
- Cancer, Hypoxia, and Metabolism
- Environmental Toxicology and Ecotoxicology
- Genetics, Aging, and Longevity in Model Organisms
- PARP inhibition in cancer therapy
- Neuroscience of respiration and sleep
- Antioxidant Activity and Oxidative Stress
- Genomics, phytochemicals, and oxidative stress
- Heme Oxygenase-1 and Carbon Monoxide
- Electron Spin Resonance Studies
- Eicosanoids and Hypertension Pharmacology
- High Altitude and Hypoxia
- Neuroinflammation and Neurodegeneration Mechanisms
- Neuroscience and Neuropharmacology Research
- Hormonal and reproductive studies
- Erythrocyte Function and Pathophysiology
- Hemoglobin structure and function
- Biochemical effects in animals
- Heart Rate Variability and Autonomic Control
- Fibromyalgia and Chronic Fatigue Syndrome Research
- Cancer, Lipids, and Metabolism
- Neuropeptides and Animal Physiology
- Carcinogens and Genotoxicity Assessment
Universidad de Jaén
2013-2024
Hospital Central Militar
2016
Secretaría de la Defensa Nacional
2016
University of Córdoba
1991-1996
The activity of several antioxidant and detoxifying enzymes, superoxide dismutase SOD, GSH peroxidase GSHPx, GSSG reductase GSR S transferase GST, the contents thiobarbituric acid reactive substances TBARS, SOD GST isoenzyme patterns were studied in livers chubs Leuciscus cephalus from reference river areas polluted urban sites. Livers fish contained higher concentrations transition metals, especially copper iron. Total GSHPx was 1.8 fold than animals, while activities TBARS content not...
Stroke remains an important health and social challenge. The present study investigated whether blood pressure (BP) parameters circulating levels of nitric oxide metabolites (NOx) adrenomedullin (AM) may predict clinical outcomes stroke. Patients (n=76) diagnosed with acute ischemic stroke were admitted to the unit history data monitored recorded. Blood plasma was collected at days 1, 2, 7 measure NOx AM levels. Infarct volume, neurological severity [on National Institutes Health Scale...
Abstract Olive oil intake has been linked with a lower incidence of breast cancer. Hypoxic microenvironment in solid tumors, such as cancer, is known to play crucial role cancer progression and the failure anticancer treatments. HIF-1 foremost effector hypoxic response, given that hydroxytyrosol (HT) one main bioactive compounds olive oil, this study we deepen into its modulatory on HIF-1. Our results MCF-7 cells demonstrate HT decreases HIF-1α protein, probably by downregulating oxidative...
Hydroxytyrosol (HT) is a bioactive olive oil phenol with beneficial effects in number of pathological situations. We have previously demonstrated that an HT-enriched diet could serve as therapeutic approach to attenuate ischemic-stroke-associated damage mice. Our exploratory pilot study examined this effect humans. Particularly, nutritional supplement containing 15 mg HT/day was administered patients 24 h after the onset stroke, for 45 days. Biochemical and oxidative-stress-related...
Poly(ADP-ribose) polymerase-1 (PARP-1) is a nuclear protein that, once activated by genotoxic agents, modulates the activity of several proteins including itself. Previous studies have established that PARP-1 inhibition may provide benefit in treatment different diseases, particularly those involving hypoxic situation, which an increased oxidative and nitrosative stress occurs. One most important transcription factors involved response to situation hypoxia-inducible factor-1 (HIF-1). The...
Poly(ADP-ribose) polymerase-1 (PARP-1) is a nuclear protein that once activated by genotoxic agents, modulates its own activity and of several other proteins. The absence or pharmacological inhibition this has been proven to be beneficial in the treatment different diseases involving hypoxic situation. We previously reported PARP-1 hypoxia-inducible factor-1 (HIF-1) response vitro, but effect not yet demonstrated vivo. brain especially susceptible injury, present study demonstrates plays...
The physiological response to hypobaric hypoxia represents a complex network of biochemical pathways in which the nitrergic system plays an important role. Previous studies have provided evidence for interplay between hypoxia-inducible factor-1 (HIF-1) and poly(ADP-ribose) polymerase-1 (PARP-1) under hypoxia. Here, we evaluate potential involvement nitric oxide (NO) cross talk these two proteins. With this aim, studied comparatively effect pharmacological inhibitors NO production or PARP...
Although cancer is multifactorial, a strong correlation between this pathology and increased oxidative stress has long been stablished. Hypoxia, inherent to solid tumors, increases reactive oxygen species should be taken into account when analyzing the response of tumor cells antioxidants. The Mediterranean diet related lower incidence cancer, particularly breast cancer. Given that hydroxytyrosol (HT) largely responsible for antioxidant properties olive oil, we have performed comprehensive...
Therapies against stroke can restore the blood supply but cannot prevent ischemic damage nor stimulate recovery of infarcted zone. The neuroglobin protein plays an important role in neuro-regeneration process after stroke; however, method for its effective systemic application has not been identified yet, as is unable to pass through blood-brain barrier. Previously, we developed different types sodium hyaluronate nanoparticles, which successfully cross barrier stroke. In this work, these...
The cerebellum is the neural structure with highest levels of nitric oxide, a neurotransmitter that has been proposed to play key role in brain aging, although knowledge concerning its contribution cerebellar senescence still unclear, due mainly absence integrative studies jointly evaluate main factors involved cell production and function. Consequently, present study, we investigate expression, location, activity oxide synthase isoenzymes; protein nitration; adult old rats.Our results show...
We have found conclusive evidence for inducible nitric oxide synthase (iNOS) activity in rainbow trout ( Oncorhynchus mykiss) tissue by means of biochemical, immunohistochemical, and immunoblotting analyses. This Ca 2+ -independent enzyme uses l-arginine to produce l-citrulline. It was significantly inhibited the analogs N ω -monomethyl-l-arginine G -nitro-l-arginine methyl ester. Kinetic analyses showed typical Michaelian behavior with no cooperative effects. The specific activities liver...