Objectives: Research has identified many factors associated with fibromyalgia (FM), but findings have been inconsistent. This study aimed to investigate changes in levels of nitric oxide (NO), inflammatory markers, lipid profile, and cortisol normal- overweight patients FM controls. Since most are overweight, we explored possible these markers according body mass index (BMI). Methods: preliminary was performed on serum samples women age-matched controls, grouped their BMI: 12 normal-weight...

The frequency of astrocytes, microglia plus oligodendrocytes, and pericytes displaying nuclei was analyzed quantified in 160-μm-wide strips the parietal cortex (Par1 region) from young aged Wistar rats. study performed on two groups rats 3–4 32–36 months. Quantifications glial cell types were made 1-μm-thick sections stained with toluidine blue. Ultrathin also to analyze ultrastructural features these cells during aging. Astrocytes increased number by about 20% 22%, respectively, age. These...

Background Different changes in neuronal and glial population of the aging brain have been described; however, degree extent these are controversial. This study evaluates quantitative cytomorphometric effects on populations parietal cortex rat. Methods The was performed two groups rats aged 4–6 30–32 months. Cortical volume, density, area, shapes soma nucleus were analyzed cortical layers I, II–IV, V, VI using serial sections stained with cresyl-fast-violet, morphometric techniques. Results...

Objectives: Fibromyalgia (FM) is a chronic disease that imposes physical, psychological, and social limitations. We have reported oxidative stress may play role in the pathophysiology of FM. Olive oil has been shown to be effective treatment against associated with several diseases. The aim this study was investigate effect olive on health-related parameters Methods: This preliminary performed blood samples 23 women diagnosed FM who consumed 50 ml organic daily for 3 weeks. Subjects were...

The cerebellum is the neural structure with highest levels of nitric oxide, a neurotransmitter that has been proposed to play key role in brain aging, although knowledge concerning its contribution cerebellar senescence still unclear, due mainly absence integrative studies jointly evaluate main factors involved cell production and function. Consequently, present study, we investigate expression, location, activity oxide synthase isoenzymes; protein nitration; adult old rats.Our results show...

Objectives: Fibromyalgia (FM) is a complex syndrome characterized by widespread pain. Its etiology unclear, and diagnosis difficult. The aim of this study was to assess plasma levels monoamine neurotransmitters (catecholamines, indolamines, intermediate metabolites) in patients with FM healthy controls investigate possible alterations the metabolism these molecules FM. We also examined potential relationships between clinical features predictive value determined receiver operating...

We have recently reported that patients with fibromyalgia (FM) may be at increased risk for cardiovascular disease. Olive oil reportedly has cardioprotective effects. examined the influence of olive consumption on factors in FM. This preliminary study was performed blood samples women FM who consumed 50 mL organic daily 3 weeks. Patients were randomized into two groups: 15 ingested extra virgin (EVOO) and refined (ROO). Cardiovascular markers measured baseline (pre measure) after (post...

Hypoxia/reoxygenation (H/R) reportedly influences nitric oxide (NO) production and NO synthase (NOS) expression in the heart. Nonetheless, a number of works have shown controversial results regarding changes that cardiac NO/NOS system undergoes under such situations. Therefore, this study aims to clarify behaviour hypoxic heart by investigating seven different reoxygenation times. Wistar rats were submitted H/R (hypoxia for 30 min; 0, 2, 12, 24, 48, 72 h, 5 days) novel approach address...

Objectives: The aim of this study was to investigate thrombosis-related parameters (blood coagulation parameters, platelet indices, red blood cell [RBC] count, and inflammatory markers) in patients with fibromyalgia (FM). Method: We carried out a case–control 35 women FM 12 age-matched healthy volunteers analyze fibrinogen levels, prothrombin time, cephaline distribution width (PDW), mean volume (MPV), RBC neutrophil-to-lymphocyte ratio, platelet-to-lymphocyte ratio (PLR). Results: results...

We have explored the innervation of rainbow trout ( O. mykiss ) liver using immunohistochemical procedures and light microscopy to detect in situ protein gene product 9.5 neuronal nitric oxide synthase immunoreactivities (PGP‐IR NOS‐IR). The results showed PGP‐IR nerve fibres running with extralobular biliary duct (EBD), hepatic artery (EHA) portal vein (EPV) that form hilum, as well following spatial distribution intrahepatic blood vessel channels. These appear single varicose processes,...

Nitric oxide (NO•) from inducible NO• synthase (iNOS) has been reported to either protect against, or contribute to, hypoxia/re-oxygenation lung injury. The present work aimed clarify this double role in the hypoxic lung. With objective, a follow-up study was made Wistar rats submitted (hypoxia for 30 min; re-oxygenation of 0 h, 48 and 5 days), with without prior treatment selective iNOS inhibitor 1400W (10 mg/kg). levels (NOx), lipid peroxidation, apoptosis, protein nitration were analysed....

A review about the possible cellular and molecular mechanisms of aging related neurodegenerative diseases.The involved in neuronal decrease, connectivity losses glial reactivity, detected both (Alzheimer's disease) physiological aging, are analyzed from morphological histological point view to provide morphofunctional base cognitive intellectual alterations characterizing senescence process. Taken together, these data correlated genetical aspects implied this process, reviewing most relevant...

Abstract To ascertain the possible implications of nitric oxide (NO • ) producing system in striatal senescence, and by using immunohistochemistry image‐processing approaches, we describe presence enzyme synthase (NOS), NADPH‐diaphorase (NADPH‐d) histochemical marker, nitrotyrosine‐derived complexes (N‐Tyr) striatum adult aged rats. The results showed neuronal NOS immunoreactive (nNOS‐IR) aspiny medium‐sized neurons nervous fibres both age groups, with no variation percentage area but a...

To date, the role that NO derived from endothelial synthase (eNOS) plays in development of injuries occurring under hypoxia/reoxygenation (H/R) lung remains unknown and thus constitutes subject present work. A follow-up study was conducted Wistar rats submitted to H/R (hypoxia for 30 min; reoxygenation 0 h, 48 h 5 days), with or without prior treatment using eNOS inhibitor L-NIO (20 mg/kg). Lipid peroxidation, apoptosis, protein nitration production (NOx) were analysed. The results showed...

Fibromyalgia (FM) is a chronic and highly disabling syndrome, which still underdiagnosed, with controversial treatment. Although its aetiology unknown, number of studies have pointed to the involvement altered mitochondrial metabolism, increased oxidative stress inflammation. The intake extra virgin olive oil, particularly one phenolic compounds, hydroxytyrosol (HT), has proven be protective in terms redox homeostatic balance reduction In this context, using proteomic approach nanoscale...

Abstract: Aims/Background: The innervation pattern of the guinea‐pig liver is similar to that human liver. However, many aspects distribution neuronal isoform enzyme nitric oxide synthase (nNOS) in and its colocalization with neuropeptides remain be elucidated. Methods: nNOS was studied fixed by light microscopic immunohistochemistry. Confocal analysis used determine neuropeptide Y (NPY) or calcitonin gene‐related peptide (CGRP). Results: nNOS‐immunoreactive (nNOS‐IR) nerves were observed...

Abstract Nitric oxide (NO) has been implicated in many pathophysiological situations the lung, including hypoxia/reoxygenation. This work seeks to clarify current controversy concerning double protective/toxic role of endogenous NO under hypoxia/reoxygenation lung by using a nitric synthase (NOS) inhibitor, novel approach address problems raised from assaults such circumstances. A follow‐up study was conducted Wistar rats submitted (hypoxia for 30 min; reoxygenation 0 h, 48 and 5 days), with...